Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,665,576	C→T	V79I (GTT→ATT)	gadX ←	DNA‑binding transcriptional dual regulator GadX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,576	0	C	T	100.0%	103.1 / NA	33	V79I (GTT→ATT)	gadX	DNA‑binding transcriptional dual regulator GadX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)

CCACTCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGT  >  NC_000913/3665334‑3665799                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 ccACTCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTa                                                                                                                                                                                                                           <  1:256048/250‑1 (MQ=255)    cTCATGTGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCGGAAAAACAGGAATCTCTCTACTACAGTGATTAACGAGTAATGAAGAAATTTTTTTAtg                                                                                                                                                                                                                         >  2:153348/1‑249 (MQ=255)                        tGATAACCGTACAAACTCGTGGTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGcctc                                                                                                                                                                                                   <  1:181843/250‑1 (MQ=255)                            aaCCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGcctcctcct                                                                                                                                                                                              >  1:192231/1‑251 (MQ=255)                                      aCTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCgg                                                                                                                                                                                    <  2:192231/251‑1 (MQ=255)                                                  ttGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAAAAGATAACAACGAAAAGATTAATGCTATTTTACGTAGCATTTCTGCATAGAGAAGATTCTTATTCTACGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAATTTTTTTTATGTGCATGACGATTTAA‑TGCATCATCCTTGAGCATCGGGAGAACTAATg                                                                                                                                                                         >  2:140977/2‑250 (MQ=255)                                                                      aaGAAGCAACGGTTTAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGAGTAATGCTCTTTTTCGTATCAATTCTGCATAGCGAAGAATCTTATTCTGCGATAGTTTGGCAATTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATTTGCATGAATATTTAA‑TGCCTCCTCCTTGAGCATCTGGAGAAATAATGAAAGCGTGTCCCTTGACACg                                                                                                                                                     <  2:288437/249‑1 (MQ=255)                                                                         aaGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCGTATTCTTCGATAGTTGCGCAACTTCCTGAAAAACAGTAATATCTCTACTACCGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGTTTCGTGAGAAAGAATGAAATCGTGTCCCTTGACACGAATa                                                                                                                                                 <  2:342822/251‑1 (MQ=255)                                                                            cagcGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGCTGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCATCCTCATTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTgcaaagaataca                                                                                                                                               >  2:246520/1‑239 (MQ=255)                                                                                     aaaGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTTCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                     <  1:374945/251‑1 (MQ=255)                                                                                      aaGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGaa                                                                                                                                                   <  1:321947/236‑1 (MQ=255)                                                                                      aaGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGaa                                                                                                                                                   >  2:321947/1‑236 (MQ=255)                                                                                        gTGCTCATCCTCAAGCAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAAATTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACCCGAATACAAATTTTTCacca                                                                                                                                   >  2:188205/1‑247 (MQ=255)                                                                                             cATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTa                                                                                                                             <  1:246520/251‑1 (MQ=255)                                                                                                     aGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTAt                                                                                                                                                                                                                          >  1:262067/1‑151 (MQ=255)                                                                                                     aGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCGCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTAt                                                                                                                                                                                                                          <  2:262067/151‑1 (MQ=255)                                                                                                       aaaaaCAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGa                                                                                                                    >  1:157838/1‑250 (MQ=255)                                                                                                              gATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCTATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCt                                                                                                            <  1:267682/251‑1 (MQ=255)                                                                                                                   ccacGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCAt                                                                                                       <  1:153348/249‑1 (MQ=255)                                                                                                                   caacGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCAt                                                                                                       <  1:188205/251‑1 (MQ=255)                                                                                                                           aGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTATGCGATAGATGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAAAGGGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑GGCCTCATCCTTGAGCATCGGGGGAAATAATGAAAGCGTGTCACTTGACAAGAATACAAACTTTTCAAGACACTGATCTACACGAATTTGGCTTGCATCAGCaaa                                                                                                >  1:114029/1‑250 (MQ=255)                                                                                                                                   gCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGt                                                                                        <  1:50691/250‑1 (MQ=255)                                                                                                                                     tctTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGCCACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCAc                                                                                     >  1:158521/1‑251 (MQ=255)                                                                                                                                        tttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGGTTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGCCCCGAATCCAAAATTTTCAACACACTTATCAACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGc                                                                                  >  2:118907/1‑251 (MQ=255)                                                                                                                                                  aTTTCTGCATAGCGAAGATTCTTATTCTGAGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTACTGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTACTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGATTGAATCCGCAAAAACCAGGtacacgcaataaaaat                                                                         >  2:165015/1‑235 (MQ=255)                                                                                                                                                                aagtttttttttctggaaTAGTGGCGAAACTTCCTAAAAAACAGGAATATCTTTACTACAGTAATAAACGAGTAATAAAAAATTTTTTTTATGTG‑AAAAAAATTTAAATCCCCCCTCCTGGAGCATGGGGAAAAAAAATGAAGGCGTGTCCCTTAACACAAATAAAAATTTTTCAACCCATTTTCCTCCCCAATTTTGGCTTCAACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAt                                                          <  1:203815/234‑1 (MQ=255)                                                                                                                                                                                 aTAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                          >  1:191823/1‑250 (MQ=255)                                                                                                                                                                                      ttcgaaaCTTCCAAAAAAAAAGGAATATTTCTATAACAGTGAGAAACGAGTAATAAAAAATTTTTTTTATGGGAATAAAAATTTAT‑TCCCCCCCCCTGGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACAAAAAAAAAATTTAAAACCCCTTTATCTACTGGAATTTGGTTTGAACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                     <  1:91612/245‑1 (MQ=255)                                                                                                                                                                                               acctaaaaaaaAGGAATATCTCTACGACAGTGAGGAAAGAGAAATAAAAAATTTTTTTTAGTTGCATGAAAATTTAA‑TGCCCCCTCCTGGAGCAGCGGAAAAAATAATAAAAGCTTGTCCCTTGACACAAAAAAAAAATTTACAACCCATTTATCTACTCAAATTTGCTTTACACCCGAAAAAACCAGGATACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTg                            <  1:191576/245‑1 (MQ=255)                                                                                                                                                                                                   gAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAacac                                                                                                                                  <  1:121993/144‑1 (MQ=255)                                                                                                                                                                                                   gAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAacac                                                                                                                                  >  2:121993/1‑144 (MQ=255)                                                                                                                                                                                                        aCAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                  >  1:382446/1‑251 (MQ=255)                                                                                                                                                                                                                         acAGTGATGAACGAGTAATGAAGAAATTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACCATTCCCATGt  >  1:268486/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 CCACTCATGGGCGATATTATTATTGATAACCGTACAAACTCGTGTTCGCATGTTCGGTTGTAAAACGTTCAGAAGCAGCGGTATAAAGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAA‑TGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGT  >  NC_000913/3665334‑3665799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑