Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,664,594 G→A L9F (CTC→TTC)  gadW ← DNA‑binding transcriptional dual regulator GadW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,664,5940GA88.9% 64.0 / ‑2.3 27L9F (CTC→TTC) gadWDNA‑binding transcriptional dual regulator GadW
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (11/13);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAGGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTATAGCTTA  >  NC_000913/3664358‑3664811
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                          
ggggggTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAg                                                                                                                                                                                                               <  1:347564/250‑1 (MQ=255)
   ggttGACTTGTTAATTAACCAACTGTCTATGCTGGTTAACCCTATATTTCGTGGTGGCTGACGAATAGTTTGTAAGTAATGGTAAACAGACAGCTCATCGAGATCCAGTCGTCGCGTATCCGGTGAACAAAACTCAACATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGACTACTCACGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATAGAATGATCGACGAATGAAGATCACCGAGCAGACAt                                                                                                                                                                                                           <  2:291110/248‑1 (MQ=255)
                   aaCAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATccc                                                                                                                                                                                          <  1:165617/251‑1 (MQ=255)
                                tGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTATGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAGGATCACCGAGCAGACATG‑AGTCATGATTATCCATTATATTTCATAc                                                                                                                                                                             >  2:192342/1‑251 (MQ=255)
                                 gCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATAc                                                                                                                                                                             <  1:138826/250‑1 (MQ=255)
                                                 ttacGTGGTAGCTGACGACTATTTTGGAAGTAATGGCAAACTGTCCGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGAAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGATATGCAGCGATCTTTTTATCTGTTCATGATAAATATCGAATGAACGGCGAATGAGAATCACCGAGCAGA‑ACGTAGTCATGAATATCCCTTATATTTACTACTACTATAatttcaatat                                                                                                                                                            >  2:205277/4‑249 (MQ=255)
                                                    cGTGGTAGCTGACGAATATTTTGTAAGTACTGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAAAAAAACGCAACATCGTCTG‑CCAAATTTTTCTCCAGAAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACAACGAATGAAGATCACCGAGCAGACATG‑ACTCATTTATATACATTATATTACATACATCGCTTatttcaatttcat                                                                                                                                                         >  2:332273/1‑250 (MQ=255)
                                                          aGCTTACGAATAGTTTGTAATGAATGGCAAACTATCAGCTAGTTGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CTAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATAGTTTTTGCTGTTCATGATAAATATCGAATTAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATATCAATTTCAGTATAc                                                                                                                                                    <  2:325632/250‑1 (MQ=255)
                                                                gtaaTATTTTGTAAGGTATGGCAAACATTCAAGTCTGCGCTATCCAGTCGTCGCGTATCCGGTGAACAATACGCAAAATCGTCTG‑CCAAATTTTTCTCCATCTGCATAATACTCTCGTTATGCTGCGATATTTTTTGCTGTTCATGATAAATATCTAATGACCTACTAATGAAGATCCCCGATCAGACATG‑TGTCATGATTATCCCTTATAGTTCATACTGCGATTATTTTAATTTCATTAGACTaatgaa                                                                                                                                             <  2:74884/249‑1 (MQ=255)
                                                                   atatTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTaatgaaatg                                                                                                                                          >  1:125016/1‑251 (MQ=255)
                                                                            aagtaaTGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGAc                                                                                                                                 <  1:2671/251‑1 (MQ=255)
                                                                            aaggaaTGTCAAACTGTCAGCGAATCGTTATCCAGGCGTCTCGTATACGGTTAGAAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCTAGCAGCAGAATACTCTCGTTATGCAGAGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACTAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTGCATACTGCGATTATTACAATTTCAGTAGACTAATGAAATGATGCCAGAc                                                                                                                                 <  2:189676/247‑1 (MQ=255)
                                                                                         cTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGTTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAgcgc                                                                                                                    >  1:13316/1‑251 (MQ=255)
                                                                                                ttCATCGATATCCAGTAGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCCGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTAATGATTATCCCTTATCTTTCATACTGCGATTATTTCAATTTAAGTATACTAATGAACTGTTGCCAGCCTATTTCTTAGCGCacaacct                                                                                                             >  2:80246/2‑245 (MQ=255)
                                                                                                     cGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCa                                                                                                         <  1:238634/250‑1 (MQ=255)
                                                                                                                      gtaatctgttaaCAAAACGAAAAATCG‑GTGTCCAAATTTTTCTCCAGCAGCTGAATACTCGCGTTATGCAGCGATAATTTTTGCTTGTCATGATAAATATCGAATGAACGACGAATGAAGATCACAGATCAGACATG‑AGTCATGATTATCCCTTATCGTTCATACTGCGATTATTTCAATTTCATTATAATAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACGGCCAGATTTGGCAGGACCaaaa                                                                                       <  2:70794/241‑1 (MQ=255)
                                                                                                                       tATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGATCGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAAt                                                                                      >  1:257123/1‑251 (MQ=255)
                                                                                                                                                         aaTTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAAt                                                     >  1:292882/1‑251 (MQ=255)
                                                                                                                                                         aaTTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGAGTATTTCAATTTCAGGATACTAAGGAAATGATGCCAGACTGTTGCGTAGCGCAAAAACTGCCAGATTTGGTAGTACCAAAATTTTTCCACAAAGTAGTTTTGCATAACAGTTAAt                                                     <  1:345192/251‑1 (MQ=255)
                                                                                                                                                                      cagcagAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTaa                                        <  1:144286/251‑1 (MQ=255)
                                                                                                                                                                               aCTCTAGTTATGCAGCGATCTTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCGGACATG‑AGTAATGATTATCCCTTATATTTCATACTGCGATTCTTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAACGTAGTTTTGCATAACAGTTACTTAATAATAAGTAAATGCAacac                               >  2:255345/1‑251 (MQ=255)
                                                                                                                                                                                ctctCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGATCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGAGTTGGt                                                                                                 <  2:22815/184‑1 (MQ=255)
                                                                                                                                                                                ctctCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGt                                                                                                 >  1:22815/1‑184 (MQ=255)
                                                                                                                                                                                ctctCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGt                                                                                                 >  1:99259/1‑184 (MQ=255)
                                                                                                                                                                                           aatcGATATTTTTTGGTGTTCATGATAAATATCGAATGAACGACGAATGAAGTTCACAGAGCAGACATG‑AGTACTGATTATCACTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGACATGATGCCAGACTGATTCTTAGAGCAAAAACTGCAAGATTTGGTAGGACCACAATTTATACACCAAGTAGTTGTGCATAACAGTTCATTGATAATAAGTAAATGCAGCACGTATATATTTTc                   >  2:5498/4‑251 (MQ=255)
                                                                                                                                                                                                          cTGTTCATGATAAATATCGAATGAACGACGAATGAAGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTATAGCt    >  1:118702/1‑251 (MQ=255)
                                                                                                                                                                                                            gTTCATGATAAATATCGAATGAACGACGAATGAGGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTTATGAAATGATGCCAGACTGTTTCTTAGCGAAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTATAGCTTa  <  1:192342/251‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                          
GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACAAAACGCAAAATCGTCTG‑CCAAATTTTTCTCCAGCAGCAGAATACTCTCGTTATGCAGCGATATTTTTTGCTGTTCATGATAAATATCGAATGAACGACGAATGAGGATCACCGAGCAGACATG‑AGTCATGATTATCCCTTATATTTCATACTGCGATTATTTCAATTTCAGTATACTAATGAAATGATGCCAGACTGTTTCTTAGCGCAAAAACTGCCAGATTTGGTAGGACCAAAATTTATCCACAAAGTAGTTTTGCATAACAGTTAATTGATAATAAGTAAATGCAGCACGAATATATTTTCGCACAGCGTATAGCTTA  >  NC_000913/3664358‑3664811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑