Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,500,129 G→A G74S (GGC→AGC)  frlA → fructoselysine/psicoselysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,500,1290GA84.6% 22.8 / ‑3.1 13G74S (GGC→AGC) frlAfructoselysine/psicoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (2/9);  total (2/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

TCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGC  >  NC_000913/3499905‑3500353
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 
ccGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAAt                                                                                                                                                                                                        <  1:149026/250‑1 (MQ=255)
        ggAAGCCAGGAACTCCAACGCAAGCTCGGATTGTGGGCCGTTCTTGCAATCGCCGGCGGGACAACCGTCGGCTCCGGTATGTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATg                                                                                                                                                                                                       <  1:228921/244‑1 (MQ=255)
           cgccagaagctccgactcAAGCCCGGGTTTTGGGCCTTTCTTGCACTCGCCGTCGGGACAACCGTCTGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGCCTTACGTTGCTCGCGTTTGTCATTGGCTGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTAGGTTTATCTGAAAAATGCCggtcgtcg                                                                                                                                                                                             <  2:91073/234‑7 (MQ=255)
                                         tGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTAGCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCa                                                                                                                                                                <  2:192389/250‑1 (MQ=255)
                                                                                                      gTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGCTAATTGTGATTCCGCAAATGTGGGGCTTTGCGGAACCATCCACCGCTTATCCGGAAAATAGAGGAGATTTTGTTTATTTGAAAAATTCCGGGAGCCCCCCGCCGGCTTTCCTCTCCGGCTGGGGCAGCCTTTGGGCCAACGATACGCCCTCATTCGCGCTTTTGGCGCTGGCGATTGtttgccatctt                                                                                                  >  1:33932/1‑241 (MQ=255)
                                                                                                                         gggCTCGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGGGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCg                                                                               <  1:141964/251‑1 (MQ=255)
                                                                                                                                            gTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAAtt                                                            <  1:188315/251‑1 (MQ=255)
                                                                                                                                                 cgtgTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTAGCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTAt                                                         <  1:126030/246‑1 (MQ=255)
                                                                                                                                                 cgcggttttCATTTGCTGGTTAAATGTTATCCCGCAAATGTGCGTCTATTCGGAACTATCCTCCGCTTATCCGGAAAATAGCTCCGATTATGTTTATCTGAAAATTGCCGTACGCCGCCCGCTGGCTTTCCTTTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTACCGATCCGTTGCTCGGTAAATTTATcg                                                       <  2:59043/243‑1 (MQ=255)
                                                                                                                                                                      aaTTGTGATCCCGCAAATGTGCGGCTATGCGGAACTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACTATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGcc                                   <  1:355977/250‑1 (MQ=255)
                                                                                                                                                                                 gtaaaatgttcttatagtcGGAACTATTCACCTCTTACCCGGAAATGAGCGACGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTGTCCTGTCCGGCTGGGCCTGCTTCTGGGCCAACGATGCGCCGTCATTGTCGAGTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTGATCGCCGCCGGAGTAATTATCGCCTTTATGTTGc                         <  2:271326/231‑1 (MQ=255)
                                                                                                                                                                                  gtaaaTGTGCGTCTATGCGGAACGATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGTATTAATTATCGCCTTTATGTTGCTac                      <  2:33932/249‑1 (MQ=255)
                                                                                                                                                                                                      aaCTATCCACCGCTTATCCGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGCTTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGACggc  >  1:49907/1‑251 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 
TCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATCCGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGC  >  NC_000913/3499905‑3500353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: