Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,481,131	G→A	L11L (CTG→TTG)	kefG ←	glutathione‑regulated potassium‑efflux system ancillary protein KefG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,481,131	0	G	A	100.0%	77.5 / NA	28	L11L (CTG→TTG)	kefG	glutathione‑regulated potassium‑efflux system ancillary protein KefG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

TCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGGCAGAAAG  >  NC_000913/3480908‑3481376                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      tCCAGCCACTCTTTAAGTAGAGCTGGGCAGCTATAGGTATAATTAGGATGCTGAAAGAAAATAACCTGGGGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATATCAATCAAAAACTCGGGATAGTGCGCGTAGAGGTCGGGAACGGTAACATTGCGGAGCTACGTGACCGGTTTAAGCAGTACACGGGTTGACACCGAGTCCTGAGCATCAGGATTGGCATACAAAAGCACAACTTTAGTTGACTGAGAcat                                                                                                                                                                                                                            >  2:281408/1‑251 (MQ=255)  cccgccccgatttCAGGAGCGTCGGTCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGGTCTCGCGCGGTAA‑GGGCTGCTCACGGGGGATATCAATAAAAAAAGCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGATTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGAcaga                                                                                                                                                                                                                           <  2:8577/241‑3 (MQ=255)       cACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGACTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATccc                                                                                                                                                                                                                      <  1:60040/251‑1 (MQ=255)              ccAGTCGCGCCGGGCAGGTATATGTTTTAGGAGTATGGTGAATTACAAACTCCTCGTGCTCTCGCA‑TAACTGCCTGCTAACGGGGGATATCAAAAAAAAAAACGGGATAGTGCGCGTAAAGGTCGTGCACGGTAATATTGCTGTGCTGTGTTGCCGGTTTAAGCGGTACCCGTTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATGCTACTCTAAAACTTTCGCTGGCTGAGATATCATCCcccgcccg                                                                                                                                                                                                               <  2:298694/250‑1 (MQ=255)                aGTAGCGCCGGGCAGATGTAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAC‑TGCATGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGAGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGACCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAAAAGCAAAACTTTCGCTGGCTGAGACATCATACACCTCCCgtg                                                                                                                                                                                                             >  2:355841/1‑249 (MQ=255)                   aGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTAt                                                                                                                                                                                                           <  1:229917/250‑1 (MQ=255)                             aGCTATAGGTATAAAGAGGATGCTGAAAGACAATCCCCTCGTGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTgtgt                                                                                                                                                                                                <  1:338462/251‑1 (MQ=255)                             aGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGACGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGCTGGGCATAAAACAGCCAAACTTTCGCTGGCTGAGACATCATCCCCCTCCGGTTAGGGTTTTTgtgt                                                                                                                                                                                                >  2:368923/1‑251 (MQ=255)                                         aaaGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGtt                                                                                                                                                                                    <  1:14119/251‑1 (MQ=255)                                          aaGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATAGCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCTCGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATTCAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGttt                                                                                                                                                                                   <  1:355841/251‑1 (MQ=255)                                             agGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTgg                                                                                                                                                                                >  1:336892/1‑251 (MQ=255)                                                                     ggtcgtgcacggTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGc                                                                                                                                                         >  1:66923/12‑250 (MQ=255)                                                                            cgcAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTAt                                                                                                                                                                                                           >  1:345661/1‑193 (MQ=255)                                                                            cgcAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACAGTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGGTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCGGAGACATCATCCCCCTCCCGGTAt                                                                                                                                                                                                           <  2:345661/193‑1 (MQ=255)                                                                              cAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGAAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGCCATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGGGCGGCAGCTCGccc                                                                                                                                                >  1:274567/1‑250 (MQ=255)                                                                                      gCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGAGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCCTCCCCCTCCCGGCATGGTTTTTGTGTATAGGCGCTGTTTTGGGCTACCATTGCGCACGGTGCGGCAGccagcccaacaaat                                                                                                                                         >  2:11355/1‑237 (MQ=255)                                                                                               cGGGGGATATCAATAAACAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACCTTTCCTTATCAt                                                                                                                               >  2:86712/1‑251 (MQ=255)                                                                                                   ggATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATGGCTGAGGTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACGTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATGATCATAATg                                                                                                                           <  2:196442/251‑1 (MQ=255)                                                                                                           aTAAAAACATCGGGCTAGTGCGCGTAAAGGTCGTGCACGGTAACATTACTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATAAATTACATTATCATAATGATAAGtt                                                                                                                    >  2:158515/1‑250 (MQ=255)                                                                                                                           aGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACAt                                                                                                    >  1:120309/1‑250 (MQ=255)                                                                                                                                          cGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCTGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGAtt                                                                                    <  1:130530/251‑1 (MQ=255)                                                                                                                                                         tGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGtt                                                                      <  1:63449/250‑1 (MQ=255)                                                                                                                                                                         cGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCACGGTGCGGAAGCTCGCCCATAAATTACATTATCATAATGATAAGTTAACATAGGCTGAACAAACGGCACCTTATGATTGAGTTCTCCTCGTTACAAATTCGTCgcggcg                                                     >  1:310552/1‑251 (MQ=255)                                                                                                                                                                                  cagatcccGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGAGTGGTTTCTCCTCTTTACAAATGCGTCGCGGCGTGCGTGTcc                                            <  2:66923/246‑1 (MQ=255)                                                                                                                                                                                     tgcccggcagtccACCGAGGTCTGAGCATCCGGATGGGCATACAACAGCTAAACCTTGGCTGGCTGAGACATCATCCCCCTCCCGGGATTGTTTTTGTGTATAGTCGCTGGTTTGGGATACCATTGCGCCCGGTGCGGCAGCTCGTCCATACATTACACTATCATAATGATAAGTTAACATAGTCTGAACAGACGTCACCTTATGATTGTTTTCTCCTCGTTACAGATTCGGCGCGGCGTGCGCGTCctg                                          <  2:63536/239‑1 (MQ=255)                                                                                                                                                                                                                          gCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGGCAGaa    <  1:368923/251‑1 (MQ=255)                                                                                                                                                                                                                           cATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTATTGTGTATAGTCGCTGTTTTGGGATAACATTGCGCCGGGTGCGGCAGCTCGCACATACATTAAATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACAGACACCATCAACCCTGGGCAGaa    >  1:331620/1‑250 (MQ=255)                                                                                                                                                                                                                             tACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCACCCGGAATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGAGTGCGCGTACTGCTGGATATGGCCACCGCCACCCACACCCCTGGGCAggagt  >  2:374022/1‑246 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      TCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAA‑TGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGGCAGAAAG  >  NC_000913/3480908‑3481376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑