Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,388,0410TG92.3% 102.9 / ‑0.5 39T50P (ACG→CCG) aaeBaromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (19/17);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

GATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGG‑AAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGC  >  NC_000913/3387799‑3388286
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                         
gATCAGCGCGGTATAACCGGCCAGCCACCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCCGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGAGGCGCAAACAGCCCAGAGAGCGAAAAGCGCCAGAAAACGGTTCACCTCCCGCAGCCAAGGCCGGAccagc                                                                                                                                                                                                                                                     >  2:245292/1‑246 (MQ=255)
    aGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGcaac                                                                                                                                                                                                                                               <  1:316183/251‑1 (MQ=255)
    aGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGAGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAAAATCCGGTTCACCTCCCGCAGCAAAGGCCGGACcggcggaaa                                                                                                                                                                                                                                                >  2:136208/1‑247 (MQ=255)
        cgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATc                                                                                                                                                                                                                                           >  1:143645/1‑251 (MQ=255)
                              cGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGc                                                                                                                                                                                                                     <  1:294708/251‑1 (MQ=255)
                                              aTTCGTCCCAGCGAGGCTATCAAGGTACAAAAACCGGCACAGATACAGCACACCTGAATCATCAATAACGGTGCGCTGAGCATCGCAATGATGATCACCAGTCCGGCAATACAGCCATTAAATGTTCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCTCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTTCCGGCCAGCGTGGCGTTTCCAGCTg                                                                                                                                                                                                     <  2:177450/251‑1 (MQ=255)
                                                 cGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGa                                                                                                                                                                                                   >  1:101990/1‑250 (MQ=255)
                                                     ccAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGa                                                                                                                                                                                              <  1:380258/251‑1 (MQ=255)
                                                       aGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGaa                                                                                                                                                                                             >  1:19861/1‑250 (MQ=255)
                                                         cGAGTATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGc                                                                                                                                                                                          <  1:210034/251‑1 (MQ=255)
                                                           aGGATATCCAGGTACAAAACCCGGCCCAGATACAGCACACCAGAATCATCTATACTGGTGCGCGGCTCCTCGCAATGATGAGCACCAGTCCGGCAAAACAGCCAGTAAATGTGCCGATGATGCGCAAAAAGCCACGATACCGAATAGCGCCAGAATACGGTTCACCACCCGCAGCAAAGGCCGGACAGCCGGCAACAACCGCCGAGTTCAGCACCGCCCACCCTGGCgctccgcggaggccgggaaatcc                                                                                                                                                                                         >  2:278267/1‑228 (MQ=255)
                                                              atatCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGGCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGGGAAAGCcaac                                                                                                                                                                                      >  2:352065/1‑250 (MQ=255)
                                                                    agggaCAAAAACAGGCCCAGATACAGCACACCAGAATCATCAATAATGGAGCGCGGATCATCGCAATGATAATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGAAAGCGAATAGCGCCAGAATCCGGTTCACCTACCGCAGCAAAGGCCGGACCGGAGGCAACAATAGACACAGTCAGTACCACCACGCGGCGCGTTTCCCTCTGGAAGTGCAAGCCaccaaacacc                                                                                                                                                                               >  2:274266/5‑249 (MQ=255)
                                                                     ggTAAAAAAACCGGCCCAGATACAGCACACCAGAATCATCAAGAATGGTGCGCGGATCACCGCAATGATGAACACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCACCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAgcg                                                                                                                                                                              <  2:101990/251‑1 (MQ=255)
                                                                            aaaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTAc                                                                                                                                                                       >  1:151187/1‑251 (MQ=255)
                                                                             aaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGTATCATCGCAATGAGGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACa                                                                                                                                                                      <  1:278267/251‑1 (MQ=255)
                                                                                       gATACAGCACACCAGAATCATCAATAATGGTGAGAGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCg                                                                                                                                                            >  1:308532/1‑251 (MQ=255)
                                                                                                       atcatcAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑GTTTACCGCaa                                                                                                                                           >  1:43519/1‑250 (MQ=255)
                                                                                                        ccatcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAg                                                                                                                                         <  2:151187/250‑1 (MQ=255)
                                                                                                          atcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCG‑CCAGATTTTaaccaaaagg                                                                                                                                        >  2:145865/1‑242 (MQ=255)
                                                                                                                  tGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTCCCTCCCAGCGAGGCGATTCCAGCTGGAAAGGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGGCCAG‑tttacagcaaaaatgatatttt                                                                                                                                >  2:85647/1‑232 (MQ=255)
                                                                                                                       cgcgGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTa                                                                                                                          <  1:295739/251‑1 (MQ=255)
                                                                                                                           gATCATAGCAATGATGATCCCCAGTCCGGCAACACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATTGCGCCAGAATACGGTTCACCCCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGTCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTAGCaa                                                                                                                      <  1:86016/251‑1 (MQ=255)
                                                                                                                                 cGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑GTTTACCGCAAAGAGAATATATTGGATAGCAATgg                                                                                                                   >  1:303057/1‑248 (MQ=255)
                                                                                                                                  gCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAACGGAAATGTAGGGTAGCAAttggga                                                                                                                >  1:102927/1‑245 (MQ=255)
                                                                                                                                                         aaTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAAGAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGTCGGTCG‑CCAG‑GTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCaaa                                                                                        <  1:225226/251‑1 (MQ=255)
                                                                                                                                                            aCAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCCCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAAcacaactc                                                                                     >  1:368735/1‑244 (MQ=255)
                                                                                                                                                                     aaaTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAT‑TTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATTCCCATCGTGATTAACCAAACTCACGCAGGc                                                                             <  1:228272/250‑1 (MQ=255)
                                                                                                                                                                     aaaTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCg                                                                            <  1:145865/251‑1 (MQ=255)
                                                                                                                                                                               atgatgCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGGGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑GTTTACCCCAAAACGAATATGTTGGTTTGCAATGGAGGAAATACCCAACGTGATTAACCAAACTCACCCACGCGATGGG‑CCAtt                                                                 >  1:314478/1‑251 (MQ=255)
                                                                                                                                                                                    gcgcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACCCACGCAGGCGATGGG‑CCATTTTACg                                                            <  1:267342/251‑1 (MQ=255)
                                                                                                                                                                                         aaaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGg‑aaga                                                      <  1:136208/251‑1 (MQ=255)
                                                                                                                                                                                                  gATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTTGCAATGGGGAAAATACCCATCGTGAGTAACCCAACTCACGCAGGCGATTGG‑CCATTTTACGG‑AAGAACGAATCCt                                             >  1:142993/1‑251 (MQ=255)
                                                                                                                                                                                                        gAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAAATCACGCAGGCGATGGG‑CCCTTTTACGG‑AAGAACGAATCCTGGCttt                                       >  1:190278/1‑251 (MQ=255)
                                                                                                                                                                                                         aatgacgcCAGAATACGGGTCCCCTCCCGCAGCAAAGGCAGGACCGGCGGCAAAAATCGCCGCTGTCAGTACCGCACAGCGTGGCGTGTCCAGCTGGAAGGGAAATCCAACCAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGGGAGCAATGGAGAAAATTCCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGG‑AAGACCGAATCCTGGCTTtc                                      <  2:43519/246‑1 (MQ=255)
                                                                                                                                                                                                                   gAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTTGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCCCGCAGGCGATGGG‑CCATTTTTCGG‑AAGAAAGAATCCTGGCTTTCGTCCCGATCt                            >  1:153364/1‑251 (MQ=255)
                                                                                                                                                                                                                           ttCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGACAGCCAACAAACAGAGCCAGTACAATGGCGGGCG‑CCAG‑GTTTACCGCAAAGCGAATATGTTGGGTAGCCATGGAGAAAATAACCAACGTGAATAACCAAAATCACGCAGGGGATGGGGCCATTT‑ACGGGAGGAAC‑AAACCTGGCTTTTGTCGCGCTCTTGTTTGcc                    >  1:71305/1‑251 (MQ=255)
                                                                                                                                                                                                                                       atcaAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGG‑AAGAACGAATCCTGGCGTTCGTCGCGATCTTGTTTGCCAGTGACcaccac        <  1:383306/249‑1 (MQ=255)
                                                                                                                                                                                                                                             ggCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGG‑AAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGc  <  1:352065/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                         
GATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCG‑CCAG‑TTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGG‑AAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGTAGC  >  NC_000913/3387799‑3388286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑