Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,096,754	G→A	G25D (GGT→GAT)	rdgB →	dITP/XTP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,096,754	0	G	A	97.4%	109.0 / ‑4.7	40	G25D (GGT→GAT)	rdgB	dITP/XTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (20/18);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

ACGGCCAGGCCAACAGTCATCTGGTGAAGTTTCTCGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGACGCGACCGATCAAAAGAATCTGCAAAAACTGCTGGAAACAATG  >  NC_000913/3096513‑3096998                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     tcgtccaggccaACAGTCATCTGGTGAAGTTTCTCGTTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTCATTAATTAGGTATCCTATGCAAAAAGTTTTCCTCGCAACCGGCCATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTCGCGACTTCGTTCTTGattt                                                                                                                                                                                                                                             >  2:109355/5‑249 (MQ=255)              cAGTCATCTGGTGAAGTTTCGCGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACa                                                                                                                                                                                                                                <  1:294898/251‑1 (MQ=255)                     tgggTGAAGTTTCTCGGTAAGAAATCCCGTGTTGCCAAAACCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAAGCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACatccctc                                                                                                                                                                                                                          <  1:313968/248‑7 (MQ=255)                                                     tGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTGGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTcc                                                                                                                                                                                                              <  1:236726/230‑1 (MQ=255)                                                     tGCCAAAAGCCAGGTGGAGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAACGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTcc                                                                                                                                                                                                              >  2:236726/1‑230 (MQ=255)                                                       ccAAAAGCCAGGGGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCtt                                                                                                                                                                                       <  1:316854/251‑1 (MQ=255)                                                              ccAGGTGGTGATTGAAAAAGGCGAACTTTGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGa                                                                                                                                                                                 >  1:169171/1‑250 (MQ=255)                                                                       gATTGAAAAAGCAGAACTTGCCCGCCACAAACAAATTAAAATCATTAACCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGTTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCTTGGCCCATACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGAtt                                                                                                                                                                       <  1:267004/251‑1 (MQ=255)                                                                           gAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTg                                                                                                                                                                    >  1:135795/1‑250 (MQ=255)                                                                              aaatgcgactttGGCCGCCACAAAAAAATAAAAATAATTAATCAGAAACAAAACCCGCCAGAAATCGCGGCGTTAATGAATTAGGTATCCTAGGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAg                                                                                                                                                                <  2:13503/241‑1 (MQ=255)                                                                               aaggcaactgtGGCCGCCACAAACAAATTAAAATCAGTAATCCGCATCAAATCCTGCCAGTAATCGCGGCGGTAATTAATTAGGTATCCTATGCAAAAAGTTTTCCTCGCAACCGGCAATTTCGGTAAAGTGCGGGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCGCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAgc                                                                                                                                                               <  2:241882/241‑1 (MQ=255)                                                                                aGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAgcg                                                                                                                                                              >  1:127252/1‑251 (MQ=255)                                                                                     aaCTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCa                                                                                                                                                         <  1:203023/251‑1 (MQ=255)                                                                                               ccACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCaaa                                                                                                                                                >  1:323219/1‑250 (MQ=255)                                                                                               ccACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGCTGTACTCGCAAACGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGACGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCaaa                                                                                                                                                >  1:26021/1‑250 (MQ=255)                                                                                                        aaTTAAAATCATTAATCCGAAAAAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCGATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCTGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCt                                                                                                                                      <  2:323219/251‑1 (MQ=255)                                                                                                             aaaTCATTAATCCGCAACAAATACCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAACGTTGTCCTCGCAACCTGCAATGTCGGTAAAGTGCGGGAGCTGGCGTCGCTGATTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTTACCTTTATCGAAAACGCGATTCTGACAGCGCGCCATGCGGCAAAAGTGAcctatttccc                                                                                                                                 >  2:273423/1‑243 (MQ=255)                                                                                                                 cATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGAc                                                                                                                                                                                          >  1:349772/1‑190 (MQ=255)                                                                                                                 cATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGc                                                                                                                              >  1:331287/1‑250 (MQ=255)                                                                                                                 cATTAACCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTGCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGAc                                                                                                                                                                                          <  2:349772/190‑1 (MQ=255)                                                                                                                     aaTCCGCAACAAATCCCGCCAGAAATCGCGGAGTTAATTAATTAGGTATCCTATGCACAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGATGAAGAAACCGGCCTGACCTTTATCGaa                                                                                                                                                                                >  1:139400/1‑196 (MQ=255)                                                                                                                      agccGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGc                                                                                                                        <  1:19100/249‑1 (MQ=255)                                                                                                                       cccGCATCAAATCCCGCCAGAAATCGTGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTGGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCAGGCGGCAAAAGTGACCGCTTTACCGGCAATTGcc                                                                                                                       <  2:301375/250‑1 (MQ=255)                                                                                                                                                gcggcgTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCCTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAAAAGACCTCGGCGTTGATTCCGCTGAAGAAAACGGACTGACCTTTATCGAAAACGCGTTTCTGAAAGCGCGCCATGCGGCGAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGAtt                                                                                              >  1:252271/1‑250 (MQ=255)                                                                                                                                                  ggcgTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCCATGTCGGTACAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAATCAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAACAGTGACCGCTTTACAGGCAATTGCCGACGACTCTGGTCTGGCGGTAGAtgtg                                                                                            >  1:334444/1‑251 (MQ=255)                                                                                                                                                            aattagttagccttggCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGgcggcg                                                                                  <  1:373505/236‑1 (MQ=255)                                                                                                                                                                      ccTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACAGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCGGGGCAt                                                                        >  1:37062/1‑251 (MQ=255)                                                                                                                                                                                 aGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCcgcgc                                                             >  1:117488/1‑251 (MQ=255)                                                                                                                                                                                 aGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCcgcgc                                                             >  1:220850/1‑251 (MQ=255)                                                                                                                                                                                               ccGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCTCTGGTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGTCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGaa                                               <  1:190516/251‑1 (MQ=255)                                                                                                                                                                                                  gCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGAc                                            >  1:298659/1‑251 (MQ=255)                                                                                                                                                                                                           gTAAAGGGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCCCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGACGCGACCGAt                                   <  2:135795/251‑1 (MQ=255)                                                                                                                                                                                                                gTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCTCAAACAGGCCTCGTCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGACGCGACCGATCaaaa                              <  2:364185/251‑1 (MQ=255)                                                                                                                                                                                                                     tGAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGACGCGCCCGCTCAAAAGAATc                         >  2:321678/1‑251 (MQ=255)                                                                                                                                                                                                                      gAGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGACGCGACCGATCAAAAGAATCt                        <  1:321678/251‑1 (MQ=255)                                                                                                                                                                                                                       aGCTGGCGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGCCGACTCTGGTCTGGCGGTGGATGTGCTTGGCGGCGCGCCGGGCGTTTACTCCGCGCGTTGTTCCGGGGAAGACCAGACCGCTTAAAAAAATCtt                       >  2:271171/1‑250 (MQ=255)                                                                                                                                                                                                                           ggCGTCGCTGCTTAGCTACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGACGCGACCGATCAAAAGAATCTGCa                     <  2:117488/249‑1 (MQ=255)                                                                                                                                                                                                                             cGTCGCTGCTTAGCGACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGACGCGACCGATCAAAAGAATCTGCaaaa                  >  1:68980/1‑250 (MQ=255)                                                                                                                                                                                                                                           gACTTCGATCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGGAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGACGCGCCCGCTCAAAAGAATCTGCAAAAACTGGTGGAAAAAAt   >  2:31319/1‑251 (MQ=255)                                                                                                                                                                                                                                            aCTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGCAAACGCTGTTCTGAAAGCGCGCCATGCGGCTAACGTGACCGCTTTACCGCCAATTTCCGACGCCTCTGGTCTGGCGGTATATGTGCTTGTCGGCGCGCCGGGCATTTTCTCCGCGCGTTATTCCGGTGCAGACGCGACCTCTCAAAAGAATCTCCATCAACTGCTGGCAACAATg  >  2:71016/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     ACGGCCAGGCCAACAGTCATCTGGTGAAGTTTCTCGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGTCTGGCGGTAGATGTGCTTGGCGGCGCGCCGGGCATTTACTCCGCGCGTTATTCCGGTGAAGACGCGACCGATCAAAAGAATCTGCAAAAACTGCTGGAAACAATG  >  NC_000913/3096513‑3096998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑