Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,934,092	G→A	intergenic (‑404/‑143)	fucA ← / → fucP	L‑fuculose‑phosphate aldolase/L‑fucose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,934,092	0	G	A	100.0%	39.2 / NA	15	intergenic (‑404/‑143)	fucA/fucP	L‑fuculose‑phosphate aldolase/L‑fucose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/9);  total (6/9)

TAACTGCAAGTTCTCTTTTTATAACCCCATTAAAAATGACCGCTCTTAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAGAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGT  >  NC_000913/2933843‑2934337                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      aaaCTGCAAGTTCTCTTTTTATAACCCCATTAAAAATGACCGCTCTTAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACaga                                                                                                                                                                                                                                                      <  1:146303/250‑1 (MQ=255)         aGTTCTCTTTTTATAACCCCATTAAAAATGACCGCTCTTAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTTTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTACCCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGttttt                                                                                                                                                                                                                                              <  1:322837/251‑1 (MQ=255)                                                 aaaaTATTTATCAAAACGGTCAGTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAATTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCaa                                                                                                                                                                                                      <  1:367588/251‑1 (MQ=255)                                                                                            tgAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTATTAAGTGTGACTGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAATTATTAACTGATATGAAGCCCGTACTCGCTGGTTTTGCACttt                                                                                                                                                           >  2:130669/2‑251 (MQ=255)                                                                                                          cGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAAcc                                                                                                                                              <  1:253248/250‑1 (MQ=255)                                                                                                                             ggTCATATGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTTAAAATGAATCCATGAGTTCATTTCAGACAGGAAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCAATTACGTGCTTAAGCTTCt                                                                                                                          >  2:363319/1‑251 (MQ=255)                                                                                                                                                           ttttCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACAt                                                                                            >  1:273797/1‑251 (MQ=255)                                                                                                                                                                      gggCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACg                                                                                 >  1:84912/1‑251 (MQ=255)                                                                                                                                                                                   tgATTATTAAAGTGATGGTATTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGt                                                                     <  2:310488/249‑1 (MQ=255)                                                                                                                                                                                   cGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGt                                                                     >  1:310488/1‑250 (MQ=255)                                                                                                                                                                                   cGATTATTAAAGGGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTg                                                                    <  1:363319/251‑1 (MQ=255)                                                                                                                                                                                              gTGAGGGTAGACACATAAATTCTCCTTCTAGCTAATAAGGGTGACCGCCGTCATATTACAAAGCGTTTTTTAGTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATaaa                                                         <  2:273797/251‑1 (MQ=255)                                                                                                                                                                                                 aTGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGAt                                                      <  1:60432/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaggtgtaccagcttCATATTACAAAGCGTTTTATATTTGAAAAATAATCCATGAGTTCATTTCATACAGGCAAATATTCACTGATAATAAGCCCTAACTCGCTGTTTTTGCACTTTTGAAAACAGAACCGATTACGTGTTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGAAGAGTTTCCTTGCTGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTc                     <  2:29684/237‑1 (MQ=255)                                                                                                                                                                                                                                                    atTACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACATTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTTCATTATTCCATTCGCGCTGCTTTGCTCACt    <  1:272758/250‑1 (MQ=255)                                                                                                                                                                                                                                                     ttACAAAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGCACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGACAAAGCAGCAGTTACATTATTCCATTCGCGCTGCTGTGATCACTGt  >  2:33401/1‑251 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      TAACTGCAAGTTCTCTTTTTATAACCCCATTAAAAATGACCGCTCTTAAAAATATTTATCAAAACGGTCATTTTTCTATTCCTCCAAGCCCGGAATGACCGTTTTCGGCACAAACAATTAATACGGTCATCTGATTTGTGTTTTTTATGATTTATTTTCTGAAACGGGCATGAAATTTCGATTATTAAAGTGATGGTAGTCACATAAAGTCACCTTCTAGCTAATAAGTGTGACCGCCGTCATATTACAGAGCGTTTTTTATTTGAAAATGAATCCATGAGTTCATTTCAGACAGGCAAATATTCACTGATATGAAGCCCGAACTCGCTGGTTTTGCACTTTTGAAAACATAACCGATTACGTGCTTAAGCTTCTGAACCTAAGAGGATGCTATGGGAAACACATCAATACAAACGCAGAGTTACCGTGCGGTAGATAAAGATGCAGGGCAAAGCAGAAGTTACATTATTCCATTCGCGCTGCTGTGCTCACTGT  >  NC_000913/2933843‑2934337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑