Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,893,647	C→T	Q331* (CAG→TAG)	ygcN →	putative oxidoreductase with FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,893,647	0	C	T	95.3%	116.6 / ‑4.2	46	Q331* (CAG→TAG)	ygcN	putative oxidoreductase with FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (20/21);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

CTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCCAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATC  >  NC_000913/2893401‑2893890                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ctgACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGc                                                                                                                                                                                                                                                  >  1:376886/1‑250 (MQ=255)   gACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCAcc                                                                                                                                                                                                                                               <  1:279438/251‑1 (MQ=255)           ttAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGcc                                                                                                                                                                                                                                        <  1:99134/250‑1 (MQ=255)                       cGGCAGAGCGCCCGCTTGTCACAAACACGGAATCACTGGAGTATGGCGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGAATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCTATTGAGCCAATACCGGAATTTCCTTGCGCGGGATGGGTATGGCGCTGGCTGCCGCACACGCGGCAGCCCAAACGCTGATAAGCGCCTGCCAGAACCGCCAGCTGCAAAATccgatt                                                                                                                                                                                                                           >  2:184170/1‑246 (MQ=255)                        ggCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCCGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTc                                                                                                                                                                                                                          >  1:260614/1‑251 (MQ=255)                          cAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCg                                                                                                                                                                                                                        >  1:13731/1‑251 (MQ=255)                                 cgcgtttATAAAAAACACGGAATCACTGGAGTAGTGTGCGCATCTGGTGCCAGAATGTGGCTTGCACAGTATGCCGGTGCACTACGCCGGTAACGGCTGGTGGCTGGTGGGCGATGCGTTGCGCATTTTCGTCAATACCGGAATTCCCGTGCGCGGCATGGATATGGCGCTGACGGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTatc                                                                                                                                                                                                                 <  2:284247/246‑1 (MQ=255)                                           aaaaaCACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGt                                                                                                                                                                                                       >  1:214469/1‑251 (MQ=255)                                              aaCACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGt                                                                                                                                                                                                       >  1:323862/1‑248 (MQ=255)                                                        cACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGGAACGGCTGACTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAACAGATGATAAGCGCCTGCTAGCACAGCGAGCATCAAACTCTGTTTCCGCTTTAGCATCACAACGTAGAGCGCAGCctg                                                                                                                                                                                          >  2:321763/1‑251 (MQ=255)                                                           tGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCAGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGGCAATACCGGAATTTCCGTGCGCGGCAGGGAGATGTCGCTGACTGGCGAGCAGGCGGGGGCACAAAAGATGGTAAGCGCCTGCGAGCCCCCCGAGCCGCAAAATATGTTTCCGCTTTGTAAACAAAACATAGAGCCCAGCctgcgg                                                                                                                                                                                       >  2:258320/1‑249 (MQ=255)                                                             gcgTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGAATGCCGTTGCAATCCGCCGGGAACGGCTGGCTGCTGGTAGTAGATGCGATGCGCAGATGCGTCAATAACGGAATTTCGGTGCGCGGCATGGAACTGGCGCTGACTGGCGCGCAGGCGGCGGCACAAGCGCTGATAAGCGCCGGCAAGCACCGGGAGCCGCAAAATCAGTTTCCGATTTCTCTTCACTACGAAGCGCGCAACCTGCtgtg                                                                                                                                                                                     >  2:105977/3‑251 (MQ=255)                                                                      gtgcatctggggctgtatgtgggCTCGCACAGTAGGCCGGTCCAATACGCGGGTATCGTCGGGCTTATGGTGGGCGGTGCTTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCCCAGGCGGCGGAACAAACGCTGATAAGCGCCTGCTAGCGCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTTTGGGAAGTTCt                                                                                                                                                                            <  2:276864/230‑1 (MQ=255)                                                                      gcgcATCTGGTGCCAGAAGGTGGCTTGCACAGTTTGCCGGTGCAATTCGCCGGTAACGGCTGGCGGCTGGTGGGCGATGCGGTGCGCAGTTGATTAAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCCCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCt                                                                                                                                                                            <  2:208913/251‑1 (MQ=255)                                                                              ggTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCg                                                                                                                                                                      <  1:27020/249‑1 (MQ=255)                                                                                 gCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGGGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATc                                                                                                                                                                 <  1:6047/251‑1 (MQ=255)                                                                                 gCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTAt                                                                                                                                                                  <  1:174595/250‑1 (MQ=255)                                                                                   cAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACACACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATc                                                                                                                                                                 >  1:47940/1‑249 (MQ=255)                                                                                                              gtcAATGCGCCGGTATCGGCTGGTTGCTGGTGGGCGATGCGTTGCGCTGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCTCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCg                                                                                                                                    <  1:336168/249‑1 (MQ=255)                                                                                                                  atccGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGAt                                                                                                                                <  1:344753/248‑1 (MQ=255)                                                                                                                    aCGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATgg                                                                                                                              >  1:224472/1‑251 (MQ=255)                                                                                                                       ccGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTAc                                                                                                                           >  1:1125/1‑251 (MQ=255)                                                                                                                          gTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGCATTCCCGTGCGCGGCATGGATATGGCGCTGACTGGAGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGCGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCCGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTAACGt                                                                                                                        >  1:373413/1‑251 (MQ=255)                                                                                                                                  gggctgcggtggcgcgtggCGTTGCGCAGTTTCGACAATACCTGAATTTCCTGGCGCGGCAGGGATCGGGCGCTGACTGTCGCGCAGGCTGCGGCTCAAACGCTGATAAGCGCCTGCTTGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGcc                                                                                                                <  2:94057/233‑1 (MQ=255)                                                                                                                                    gatgctgGTGGGCGATGCGTTGCGCAGTAGCGTCAATACCGGACTTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCAAGCCGCCACGTCTGTTTCCGCTTTATCACAACACCGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCATTATCAGCATGTTCCGGCGCGTCTGCATCGCCCGGGCTGGTACCGTACGTGGCCTg                                                                                                              >  2:76891/3‑251 (MQ=255)                                                                                                                                        ctgGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGtt                                                                                                          >  1:231289/1‑251 (MQ=255)                                                                                                                                         tggtggGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCCCCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGtt                                                                                                          <  1:136012/250‑1 (MQ=255)                                                                                                                                                             aGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCcgcg                                                                                      >  1:265167/1‑250 (MQ=255)                                                                                                                                                                tGCGTCAATACCGGAATTTCAGTACGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCGAGCACCGCGAGCCGCAAAATCTGATTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGGTCTACAGCGTTATCAACATGTTCCGGCGCTTTTGAAACGCCCGGGATGGTCCCGTCCGTGGACTGCGTTAATGCGGGCACTTTCCCGCGAttt                                                                                  >  2:221283/1‑251 (MQ=255)                                                                                                                                                                               aTTTCCGTGCGCTGCATGGATATGGCGCTGCATGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATATGTTTCCGCTTTATCATCACAACGAAGAGCGCAGCCTGCTGTGGGCTGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCATGCGTTAATGCAGGATCTTTCCGTCGGTTTATGGGATCAggtgg                                                                    >  2:294717/1‑247 (MQ=255)                                                                                                                                                                                   ccGTGCGCGGCATTGAGATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTAACGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATaaa                                                               <  1:31704/251‑1 (MQ=255)                                                                                                                                                                                     gTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATCAGCGCCTGCTAGCACCGCGAGCCGCACAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCg                                                                                                                                                      >  2:332166/1‑162 (MQ=255)                                                                                                                                                                                     gTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCg                                                                                                                                                      <  1:332166/162‑1 (MQ=255)                                                                                                                                                                                                ggATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGc                                                  >  1:207815/1‑251 (MQ=255)                                                                                                                                                                                                 gATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCt                                                 <  1:13033/251‑1 (MQ=255)                                                                                                                                                                                                        cgcTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAg                                          <  1:238134/251‑1 (MQ=255)                                                                                                                                                                                                         gcTGACTGGCGCGCGGGCGGCTGCTCAACCCCTGATAAGCTCCTGCTAGCACCGCTAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGATCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTTGCCTGCGTTAATGCAGGATATTTCCcgc                                                                                       <  1:27713/205‑1 (MQ=255)                                                                                                                                                                                                               tGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCCAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGCAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCt                                   >  1:189977/1‑251 (MQ=255)                                                                                                                                                                                                                ggCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTc                                    <  1:365796/249‑1 (MQ=255)                                                                                                                                                                                                                           agttcaaacgggtgcatttccgatcTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTAACATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGACCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAc                       <  1:375918/227‑1 (MQ=255)                                                                                                                                                                                                                                 acaAACGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACgtcgtc                 >  1:269024/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaCGCTGATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACg              <  1:245402/251‑1 (MQ=255)                                                                                                                                                                                                                                          gATAAGCGCCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtg         <  1:231389/250‑1 (MQ=255)                                                                                                                                                                                                                                              aGCGCCTGCTAGCACAGCGAGCCGCAAAATCTGTGTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACAGTACGTGGACTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGGGATAAACCTGTTCCACCGCGGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGcc    >  2:117516/1‑250 (MQ=255)                                                                                                                                                                                                                                                cgcCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATc  >  1:277481/1‑251 (MQ=255)                                                                                                                                                                                                                                                cgcCTGCTAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGACTGTGGCATc  >  1:12191/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CTGACTCGCTTTAAAGCGCATCCGGCAGTGCGCCCGCTTATCAAAAACACGGAATCACTGGAGTATGGTGCGCATCTGGTGCCAGAAGGTGGCTTGCACAGTATGCCGGTGCAATACGCCGGTAACGGCTGGCTGCTGGTGGGCGATGCGTTGCGCAGTTGCGTCAATACCGGAATTTCCGTGCGCGGCATGGATATGGCGCTGACTGGCGCGCAGGCGGCGGCACAAACGCTGATAAGCGCCTGCCAGCACCGCGAGCCGCAAAATCTGTTTCCGCTTTATCATCACAACGTAGAGCGCAGCCTGCTGTGGGATGTTCTACAGCGTTATCAGCATGTTCCGGCGCTTTTGCAACGCCCGGGATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATC  >  NC_000913/2893401‑2893890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑