Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,851,821 G→A G274D (GGC→GAC)  hypB → hydrogenase isoenzymes nickel incorporation protein HypB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,851,8210GA96.3% 70.7 / NA 28G274D (GGC→GAC) hypBhydrogenase isoenzymes nickel incorporation protein HypB
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (8/18);  minor base C (0/1);  total (8/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAG  >  NC_000913/2851576‑2852053
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        
cGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAAACACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAATTGACCTGTTGCCGTATCTCAACTTTTACGTTGAAAAAAGCATCGCCTGCGCCCGCGAAAGCAATCCAGAAATTGAAATCATCCTTATTTTCGCCACCAGcgacga                                                                                                                                                                                                                                       >  1:313194/1‑249 (MQ=255)
         ccTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTAGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGAcc                                                                                                                                                                                                                            <  1:186970/251‑1 (MQ=255)
             ggtggCCCTGCCAGCCTCGATCTCGGTGAAAAACCCAACGTGGCGGTGCATTCCGTTGCCGAAGCTGAAGACAAACCACTGAAATAACCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTACCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGGCAATCCAGAAATTGAAATCATCCTTATCTCCGCCACCAGCGACGAAGGCATGGActagga                                                                                                                                                                                                                        <  2:12416/247‑6 (MQ=255)
                    cGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGGGAAGACAAACCACTGAAATATCCGCCTATGTTTGCCGCCGCCCCCCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCCACTTTGACGTTTGAAAGGGCATCCCCTGCGCCCCCGCAGTCAATCCAGAAATTGAAATCATCCTTATTTTCCCCCCCCGCGACGAAGGGAGGGGCCAGTGGCTGAAc                                                                                                                                                                                                                 >  1:48825/1‑251 (MQ=255)
                        cAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAActggc                                                                                                                                                                                                             <  1:75263/251‑1 (MQ=255)
                         aGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAActggct                                                                                                                                                                                                            <  1:19892/251‑1 (MQ=255)
                                    cGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGAcaca                                                                                                                                                                                                   <  1:85006/249‑1 (MQ=255)
                                                           tGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAACTTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGcc                                                                                                                                                                          >  1:235877/1‑251 (MQ=255)
                                                                 ccGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATcc                                                                                                                                                                    <  1:84061/251‑1 (MQ=255)
                                                                 ccGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGGTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATcc                                                                                                                                                                    <  1:370573/251‑1 (MQ=255)
                                                                                                    ccGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTGCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt                                                                                                                                  <  1:344989/250‑1 (MQ=255)
                                                                                                                             tGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTTAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGAATGGACAAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGCTCCGCACCATTGCCGGCAACCAGGCGAACGTCGACGTCTGTGGAATTCAGCGCGGTGTCgctt                                                                                                        >  2:91313/1‑248 (MQ=255)
                                                                                                                              gctgctgattaacaaAGTTGACCTGTTGCCGTATCGTAACTTTGACGTTGAAAAGGGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAATTCATCCTTATTCCCTCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTTGTTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGAtt                                                                                                        <  1:15688/240‑1 (MQ=255)
                                                                                                                                        aacaaAGGTGACCTGTTGCCGTTACTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAACTGAAAGCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCCCCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTc                                                                                             <  2:48825/251‑1 (MQ=255)
                                                                                                                                         acaaAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCg                                                                                            >  1:155739/1‑251 (MQ=255)
                                                                                                                                                gtaCCTGGTGCCGTATCCCAACTTTGATGTTTAAAAGTGCATCGCCCGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTAGTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTATTCGGCATCTg                                                                                     <  2:256904/249‑1 (MQ=255)
                                                                                                                                                 gACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCTACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTg                                                                                     <  1:131093/250‑1 (MQ=255)
                                                                                                                                                  aCCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAAGTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTTGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCTCCATTGACGGCAACCAGTCGAAATTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCg                                                                                   <  1:266423/251‑1 (MQ=255)
                                                                                                                                                                                        atccccTGCGCCCGCGAATTCAATCCATAAATTTAAATCATCCCTGTTTCCGCCACCATCGCCGAAGGGATGGACCAGTGGCTGGACGGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAGCGGTCAGCCGCGCGTGGGCCAGTGGGTACTg                                             <  2:313194/247‑1 (MQ=255)
                                                                                                                                                                                                         aggtaaTCCAGAAATTGAAAACATCCTTATTTCCACCACCCGGTACGAAGGTATGGACCAGTTGTCGAGCTGGCGGGACACACATCGATGTGCATATGCGGTCCCGGACAGATCCGCACCATTGCCGGCATCCAGGCGAAAGTCTACATCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCTGCTGCGATGAAAACGGTCAGCCGCGAGTGGGCCAGTTGGGACTGGTACACGTTGGCTTTGc                            <  2:290905/247‑1 (MQ=255)
                                                                                                                                                                                                            cAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCa                          <  1:91313/250‑1 (MQ=255)
                                                                                                                                                                                                                 ccgaccttGAAATCACCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCg                    <  2:202606/245‑1 (MQ=255)
                                                                                                                                                                                                                  aGAAATTGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCCCCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAg                                                                                                                     >  1:28546/1‑153 (MQ=255)
                                                                                                                                                                                                                  aGAAATTGAAATCATCCTTATGTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGAGCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTTTGCGGCATTCAg                                                                                                                     <  2:28546/153‑1 (MQ=255)
                                                                                                                                                                                                                        tGAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCg                    >  1:202606/1‑244 (MQ=255)
                                                                                                                                                                                                                         gAAATCATCCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGtaattaa             <  1:261348/250‑1 (MQ=255)
                                                                                                                                                                                                                              catcCTTATTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGc       >  1:59649/1‑251 (MQ=255)
                                                                                                                                                                                                                                     aTTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAg                      <  1:70196/229‑1 (MQ=255)
                                                                                                                                                                                                                                     aTTTCCGCCACCAGCGACGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCAGAGGCGTTCCCGGCCAGATCCGCAACATTGACGGCAAACAGGCGAAAGTCGACGTCTGCGGCATTCAGCTCGATGACGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCCCGTGGGCCAGGGGGTACGGGTCCACGGTGGCTTTGCCATGAgagatcggtgaaggggcgtgg  >  2:70196/1‑229 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        
CGTTGGCAACCTCGTATGCCCGGCCAGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGACAAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTTGACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAG  >  NC_000913/2851576‑2852053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: