Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,774,947	G→A	G544S (GGT→AGT)	yfjW →	uncharacterized protein YfjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,774,947	0	G	A	90.9%	23.5 / ‑5.4	11	G544S (GGT→AGT)	yfjW	uncharacterized protein YfjW
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (6/4);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTC  >  NC_000913/2774744‑2775119                                                                                                                                                                                                            |                                                                                                                                                                             ggCCAGAATCGGAGTTTATTATTTCTGAAGGGAGTTGGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGATTTTGTTGATAACAAAATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTAGATATTTGGTTTAAGTCTGCTAGCACTTTCTATAAAATTCATTCATTCTTCCTTTAAGatt                                                                                                                               >  2:290880/1‑249 (MQ=255)        aTCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGAtgtg                                                                                                                         >  1:210250/1‑250 (MQ=255)                         cTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTg                                                                                                       >  1:23403/1‑251 (MQ=255)                                  ggttGTATTGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCAtt                                                                                              <  1:214112/249‑1 (MQ=255)                                                        aaGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGCATGAAAATTTATTCTTGTAACGCATTTGGCTCCATTTGGAGCCttttt                                                                        >  1:265868/1‑251 (MQ=255)                                                                            atGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACttt                                                                                                                                     >  1:288877/1‑170 (MQ=255)                                                                            atGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACttt                                                                                                                                     <  2:288877/170‑1 (MQ=255)                                                                                   agcagATCTTGTTTTTTTTGATAACAAGATTATGAACTTTTGTAAAATTACTTATTTGCAATATAAGCGAGATATGTGTGGTTCTTTTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCGATCAATTTCTATAAAATTCTTTCATTCTTACTTTTAGAATGATGTGAATGAAAATTTATTCTTGTATCGCATTTGGCTCCAATTGGAGCCTCTTTATTTCTATTTATTAAACTATCCTAAGt                                             <  2:330267/251‑1 (MQ=255)                                                                                      agATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCACTCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCta                                                                                                                                                            >  1:272023/1‑137 (MQ=255)                                                                                      agATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAATCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCaaggtcta                                                                                                                                                            <  2:272023/137‑7 (MQ=255)                                                                                                                             tAAAATAACTTATTTGCAATCTAAGCTATGAATTTTAGGATCTTATATTATGTATCTATTAA‑GTCTATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTAATTAATTCTTACTTTAAGAATGATGTTATTGAAATTTTATTCTTGTAACGCATTTAGCTCCAATTGGATACTTTTTATGAATATTTATTAAACTTACCTAAGTTAAACCTGTTTTTCAAGCAGGCTTGAGCGTggagagaacacac  >  2:375902/1‑239 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                             GGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTC  >  NC_000913/2774744‑2775119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑