Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,684,686 A→C V274V (GTT→GTG glyA ← serine hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,684,6860AC93.8% 69.7 / ‑3.6 32V274V (GTT→GTGglyAserine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (17/13);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01

GATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGTGGTGGTAGTAACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGAT  >  NC_000913/2684451‑2684931
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      
gATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCg                                                                                                                                                                                                                                         >  1:197598/1‑251 (MQ=255)
  tCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGAt                                                                                                                                                                                                                                       >  1:262926/1‑251 (MQ=255)
    gTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGACGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACAATCGCTTTAGCGTTTTTAGCGACATGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCCCCGCTTTACCGGCGAtt                                                                                                                                                                                                                                      >  2:37132/1‑250 (MQ=255)
                gTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTAACGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTGCAGAGCCACCGCTTTACCGGCGATTACGAGCATAAACg                                                                                                                                                                                                                         >  1:5703/1‑251 (MQ=255)
                     tgttgACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGATTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCg                                                                                                                                                                                                                    >  2:62653/1‑251 (MQ=255)
                                atgctAGCACGGCCCATAGCGGCGTCTGCTGCTTTACCGTTCAGTTTTTTCTTAACCAGACCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCTCTCTCGAGGAACACTTCTACCCTCGCCTTAGCGGTTTTAGCGACCTTCTGCTGTTAAGTTTTGAACTCATGCTCCATCGCTTCTTTCATAGCCACCTCGTTACCGGCGATTCCGTGCATCAACGTTCCGCCCTGaccac                                                                                                                                                                                                          <  2:362163/246‑1 (MQ=255)
                                         tggCCAAGAGAGGCGTCTGCTTTTTTACCGGTCAGGTTTTTCTCAAACAGCTCAACCAGGAAAAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGTGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGATGGAAAGTTTTGACATCAGGCACCATCGCTTCTTTCAGAGCCACCGCGTTACCGGCGATTACGTGCCTCACCTGATCGCCGCGACAACCAGGGACAAc                                                                                                                                                                                                >  2:160764/2‑251 (MQ=255)
                                             ccAGTGCGGCGTCTGCTGCTTTACCGGTCAGGTTTGTATCAACCAGAGCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACGTTGTAGCCGCGCTCGAGTAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCGGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCa                                                                                                                                                                                            <  2:379946/251‑1 (MQ=255)
                                                                     cGGTCAGGTTTTTATCAACCAGATAAACCAGGAACAGGAGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTAAGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGctct                                                                                                                                                                   >  1:312818/1‑251 (MQ=255)
                                                                       gTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTc                                                                                                                                                                 >  1:173963/1‑251 (MQ=255)
                                                                                    caaccagatcaaccagTAACAGGTGGTTATCAGTGACGCCGGAAACCACTTTGTAACCGCGTTCGAGGAACTCTTCTTCCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCAGCGCTTCTTTCAGAGCCTCCGCTTTACCGGCGATTACGTGCATCAACGGCCCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTCGTACAGCTTATCGCTACCTCCTTTc                                                                                                                                                    <  1:285049/251‑1 (MQ=255)
                                                                                          gtgcaaccagGAACAGGGGGTTATCAGGTCCGCCGGAAGCCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCTCCAgg                                                                                                                                              <  2:320638/248‑1 (MQ=255)
                                                                                                  agGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACATCTCTTCGCTACCACCTTTCGCCAGGATCAGgcc                                                                                                                                      <  1:302176/251‑1 (MQ=255)
                                                                                                      aCAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGctc                                                                                                                                                                    >  1:347900/1‑217 (MQ=255)
                                                                                                      aCAGGGGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTATCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCGCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCATCGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGctc                                                                                                                                                                    <  2:347900/217‑1 (MQ=255)
                                                                                                          ttggtTATAAGAGCCGCCGGAAACCACTTTGTATCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCACAATCGCTTCTTTCAGAGCCACCGCTTTACAGGCGATTGCGTGCATCCCCGTACCGCACTGACCACAAGGGAAACAGAAGGAGTTCAGTTATTTGCACAGCTCTTTGCTACCACCTTTCGCCAGGATCACGCAGCcgcgcc                                                                                                                              >  2:83689/2‑250 (MQ=255)
                                                                                                          gtggtTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCcgcgcg                                                                                                                              <  1:321397/251‑1 (MQ=255)
                                                                                                             gtTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTATACCATCGCTTTAGCGTTTTTAGCGACCGGCTGCTGGAAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGACTACGTGCAACAACGGACCGCCCTGACCAACAGGGAAAACGGCAGAGTTCAGCTTTTTTTACAGCTCTTCCCTACCACCTTTCACCAGGATccagacaccgagcgacc                                                                                                                           >  2:96237/1‑235 (MQ=255)
                                                                                                                    gTGCCGCCGGTAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTCCAGAGCCACCGCTTTAACGTCGATTACGTGCCACAAAGGACCGCCCTGACCACCTGGGCAAACGGCAGAGGTCAGTTTTTTGTACAGCTCTTCGCTAACACATTGCGCCAGGGTCAGgccacagcggggccacgccgg                                                                                                                    >  2:201939/1‑233 (MQ=255)
                                                                                                                    gTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGcc                                                                                                                      >  1:198238/1‑249 (MQ=255)
                                                                                                                        cgccgGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGACACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGGCCCGCCAGGGtt                                                                                                                >  1:252117/1‑251 (MQ=255)
                                                                                                                                      ttGTAGCCGTGCCCGAGGAACACTTCTACCATCGCTTTAGCGTTGTGAGCGACCTGCGGCTGGAAAGTTTTGAACTCAGCCTCCACCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACTTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCCCCTTTCGCCAGGTTCAGGCCGCCGCGCGGTCCCGCCAGGGTTTTGTGAgtggt                                                                                                     <  1:145008/248‑1 (MQ=255)
                                                                                                                                      ttGTAGCCGCGCTCGAGGAACACTTCTACCATCGTTTTAGCGTTTTTAGCCACCTGCTGCTGTTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCCCCGCTTTCCCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGATTTCAGTTTTTTGTACAGCTCTTCGCTACCCCCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGtggtgg                                                                                                   <  1:259702/250‑1 (MQ=255)
                                                                                                                                               tgcTCGAGGCACACTTCACCCAACGCTTTAGCGTTTTTAGCGACCTGCTGCTGGAAAGTTTTCAACCCAGAATCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGTTTACGTACATCACCGGACCGCCCGGACCACCATGGAAAACGGCCGAGTCCAGTTTTTTGTACAGCTCTTCGCTCCCCCCATTCGACAGGATCCGGCCGCCGGGCGGACCCGCCAGGCTTTTGAGAGTGAGGGCAGTaacacc                                                                                         >  2:253209/2‑249 (MQ=255)
                                                                                                                                                gcTCGAGGAACACTTCTACCATCGCGTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGACCTCAGTCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTTTACAGCTCTTCGCTACCCCCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGTGGTGGTAGTAACAACg                                                                                        <  1:236547/251‑1 (MQ=255)
                                                                                                                                                   cgaggaaacctgcttccATCGTTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCCCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCTCCAGGATCAGGCCGCCGCGCGGCCCCGCCAGGGTTTTGTGAGTGGTGGTAGTAACAACGTGa                                                                                     <  1:334528/236‑1 (MQ=255)
                                                                                                                                                           acTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCCGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTACGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGTGGTGGTAGTAACAACGTGAGCATGAgg                                                                             >  1:126816/1‑251 (MQ=255)
                                                                                                                                                                            ggCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGCGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGTAAAACGGCAGAGTACAGTTTTTTGTACAGCTCTTCGCTAACCCCTTACGCCAGGATCAGGCCTCCGCGCGGGCCCGCCAGGGGTTTGTGCTTGGGTGTAGGAACAACGTGAGCATGAGGAACCGGGGTCggga                                                               >  2:18791/2‑247 (MQ=255)
                                                                                                                                                                                    gagcgacctgtggcggttaatttttGAACTCAGGCTCCACCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGGTCTGCAACGGCCCGCCCTACCCCCCAGGGAAAACGTCAGAGTTCATTTTTTTGCACAGCTTTTCGCTCCCCCCTTTCGCCAGGATCAGCCCGCCGCGCGGAACCGCCAGGGTTTTGTGAGTGGTGGTAGTAACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACTCcagca                                                    <  2:318721/230‑1 (MQ=255)
                                                                                                                                                                                            tgctgctgGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGCCCCGCCAGGGTTTTGTGAGTGGTGGTAGTAACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAAcc                                              >  1:333164/1‑249 (MQ=255)
                                                                                                                                                                                                                  cAGGCTCCATCGCTTCTTTCAGAGCCACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGGTTTGTGAGTGGTGGTAGTAACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATc                       >  1:97518/1‑250 (MQ=255)
                                                                                                                                                                                                                                      agagCCACCGCTTTACCGGCGATTGCGTGCACCAACGGACCGCCCTGACCACCAGGGAAAACGGAAGAGTTCAGTTTTTTGTTCAGCTCTGCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGGGGACCCGCCAGGGTTTTGTGAGTGGTGGTAGTAACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGAt  <  1:366450/251‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      
GATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGGCCCAGAGCGGCGTCTGCTTCTTTACCGGTCAGGTTTTTATCAACCAGATCAACCAGGAACAGGTGGTTATCAGTGCCGCCGGAAACCACTTTGTAGCCGCGCTCGAGGAACACTTCTACCATCGCTTTAGCGTTTTTAGCGACCTGCTGCTGGTAAGTTTTGAACTCAGGCTCCATCGCTTCTTTCAGAGCAACCGCTTTACCGGCGATTACGTGCATCAACGGACCGCCCTGACCACCAGGGAAAACGGCAGAGTTCAGTTTTTTGTACAGCTCTTCGCTACCACCTTTCGCCAGGATCAGGCCGCCGCGCGGACCCGCCAGGGTTTTGTGAGTGGTGGTAGTAACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGAT  >  NC_000913/2684451‑2684931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: