Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,632,711	G→A	R454C (CGT→TGT)	guaB ←	inosine 5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,632,711	0	G	A	97.5%	107.6 / NA	40	R454C (CGT→TGT)	guaB	inosine 5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (26/13);  total (26/14)
Fisher's exact test for biased strand distribution p-value = 3.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATAC‑CACGGTAAGATTTGTAAGAACGGCCCTGGTAGAG  >  NC_000913/2632493‑2632945                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            aGTCCAGAATGCGGCTGCGATGCTTCTGAATGTTTTCCGTCATTGACGCTTATTCCGAGGAAAGTGAAACAGATAATATAAATCGACCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACAGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACATACTCCGCTTTAGTACACAGTTCGTCGATaagaacggaagagaggcggg                                                                                                                                                                                                             >  2:43928/1‑232 (MQ=255) aGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGGCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATa                                                                                                                                                                                                                                <  1:43928/232‑1 (MQ=255)  gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGAcc                                                                                                                                                                                                            <  1:118108/251‑1 (MQ=255)                  cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAgg                                                                                                                                                                                            >  1:249504/1‑251 (MQ=255)                    atgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGAACCACAGCCGGTCAGACCCATACAGGAGCGCAGgcc                                                                                                                                                                                          >  2:76498/1‑251 (MQ=255)                          aTGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTCCAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCAt                                                                                                                                                                                    >  1:350096/1‑251 (MQ=255)                               tGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATAAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATctgct                                                                                                                                                                               >  1:347167/1‑251 (MQ=255)                               tGTTTTCCGTCATAGACGCTTATTCAGAGGCAAGGGCCAAAGATACTATAAATCGACCGACATG‑AAGTCGGGCGAAGAGAATAAGGAGCCACGACAGTAGTTCGGGGACTCTTTAGTAATGGACACGTCGTGACAGTGGCTTTCCTGAATACCCGCACCGCTGATACGTACACACTCCGCTTTAGTACACAGTTCGTCGATCGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGTCCACCCATatgct                                                                                                                                                                               >  2:49200/1‑251 (MQ=255)                                gTTTTCCGTCATTGACGCTTAGTCCGAGGCAAGTGAAACAGATAATATAAACCGCCCTACATG‑AAGTCGGGGGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGGCGATAGTACCACAGCCGGGCAGACCCATACAGGAGCGCAGGCCGCCCATctgct                                                                                                                                                                               <  2:249504/250‑1 (MQ=255)                                    tCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTg                                                                                                                                                                           >  1:173995/1‑250 (MQ=255)                                      cGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAAt                                                                                                                                                                        >  1:330213/1‑251 (MQ=255)                                         cagtGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGa                                                                                                                                                                      <  1:8947/247‑1 (MQ=255)                                             gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGAGCAGGCCGCCCATCTGCTGGTGAATGATctct                                                                                                                                                                 >  1:232955/1‑251 (MQ=255)                                                           gggaaGTGAAACAGATAATATAACTCGCCCG‑CATGGAAGTCGGGCGAAGAGAACCAGGAGCCCAGGCGTTAGGTCGGGGATTCTTTAGTAATGGTCACGTCTTGAACGGGGCTTTCCTGAATGCCCGCTCCGCTGATACGTACAAACTCCGCTTTAGTCCACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCttt                                                                                                                                                   <  2:367204/248‑1 (MQ=255)                                                                   aaaCAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCt                                                                                                                                            <  1:191603/250‑1 (MQ=255)                                                                   aaaCAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCAGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCt                                                                                                                                            >  2:191603/1‑250 (MQ=255)                                                                       aGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGgccgcc                                                                                                                                                                                       >  1:376893/1‑203 (MQ=255)                                                                       aGATAATATAAAACGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGTAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGTCCCATACAGGAGCGCAGgccgcc                                                                                                                                                                                       <  2:376893/203‑1 (MQ=255)                                                                        gATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGACCAGACGGTAGTTCGGGGACTCTTTAGCAATGGTAACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGATGCTACATAAAAACTCCGCTTTAGTACACAGTTCGTCGCTAGTACCACAACCGGTCAGACCCATACCGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTAcgcg                                                                                                                                       >  1:167858/1‑250 (MQ=255)                                                                              ataAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCg                                                                                                                                >  1:179590/1‑251 (MQ=255)                                                                                         tccATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTAGGGGACTCTTTAGTACTGGTCAAGTCGTGAAAGTGGCTTTCCTGAATGCCCTCACCGCTGAGACGTAAAAACTCCGCTTTCGTACACAGTTCGTCGATAGTACCACAGCCAGTCAGACCCATACAGGAGCGCAGACCGCACCTCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTCTAGGCTACGCGCCATTCGATACCTTCCgg                                                                                                                     >  2:237269/3‑251 (MQ=255)                                                                                          aCATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGc                                                                                                                    >  1:298898/1‑251 (MQ=255)                                                                                                     gggCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGt                                                                                                          >  1:208964/1‑251 (MQ=255)                                                                                                             agaATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCg                                                                                                  >  1:193345/1‑251 (MQ=255)                                                                                                               aaTCAGGAGCCCGGACGTTTGTGTGGGGACTCTTTAGTAGTGGTCACGTCGAGAACGTGGCTTTCCTGAATGCCCGCCCCGCTGATTCGTCCAAACTCCGCTTTAGTACACCTTTCGCTGTTAGTACCACAGCCGGTCAGACCCATTCAGGAGCGCATGCCGCTCATCTGCGGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTTTCGTCAGCGtt                                                                                                <  2:86224/251‑1 (MQ=255)                                                                                                                           agccgggaGTTCGGGTACTCTTTATTAATGGTCACTTCGTGAACGTGGCTTTCCTTAATGCCCGCTCCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTGCCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAAGGATCTCTTTCAGGCGGCCTTTATAGGCTACGCGACCTTCGATTCCTTCCGGCACCAGTTTGTCGGCAGCGGTATCGGTCTGGaa                                                                                    <  2:193345/244‑1 (MQ=255)                                                                                                                                                    tAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGAc                                                           >  1:220163/1‑251 (MQ=255)                                                                                                                                                     aaTGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGAc                                                           >  1:135128/1‑250 (MQ=255)                                                                                                                                                                 gtGAACGTGGCTTTCCTGAATGCCCGCCCCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTGCCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCGTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATcg                                                       <  1:358656/242‑1 (MQ=255)                                                                                                                                                                   gAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGa                                             >  1:115000/1‑250 (MQ=255)                                                                                                                                                                     ccGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCTATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCATTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAAcc                                          <  2:298898/250‑1 (MQ=255)                                                                                                                                                                             ttCCTGAATTCCCTCCCCACTGATACGTACAAACCCCGCTTTAGGACACAGTTCGCCGATAGTCCCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATAc‑c                                   <  2:135128/249‑1 (MQ=255)                                                                                                                                                                                      gCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCAATCTGCTGGGGAATGATCTCTTTCAGGCGACCTTTATAGGCTACACGACCTTCTATACCTTCCGGCACCATTTTGTCGGCAGCGTTATCGCTCTGGCAATAACGGTCAGAGGCACCTTTGGACTACGCTCCCCGGGAACCCATCC‑CACGGAAAgctt                        >  2:286388/1‑248 (MQ=255)                                                                                                                                                                                                 tGATACGTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATAC‑CACGGTAAGATTTGTAAGAACgg             >  2:195091/1‑251 (MQ=255)                                                                                                                                                                                                     acttACAAACTCCTCTTCAGTACGCAGTTCGTCTATATAACCACAGCCGGTCAGACCCAGACAGTAGCGCAGACCGCCCATCTGCTGTTGAAGGATCTCTTTCAGTCGACCTTTATAGGCTACTCGACCTTCTATACCTGCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTTGAAATAACGGTCAGAGGAACCTTTGTACCTCGCTCCCAGGGAACCCATAC‑CACGGTAAGATTTGTAAGAACGGcca          <  2:350096/247‑2 (MQ=255)                                                                                                                                                                                                       gTACAAACTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATAC‑CACGGTAAGATTTGTAAGAACGGCCCTgg       >  1:126144/1‑251 (MQ=255)                                                                                                                                                                                                       gTACAAACTCCGCTTTAATACACAGTTAGTCGATAGTACTACAGCCGGTCCGACCCATACAGGAACGCAGGACGCCCATCTGCTGGTGCATGATCTCTTTCAGGCGCCCTTTATAGGCTCCACGCCCTTCGATAACTTCAGGCACCAGTTGATCGGCAGCGTTATCGCGCTGGCAGTAACGGTCAGGGAAACCTTTGTGCATCGCGACCAGTGAACCCATAC‑CACGGTAAGATTTGTAAGAACGACCCTgg       >  2:190083/1‑251 (MQ=255)                                                                                                                                                                                                         acaaacgtcGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATAC‑CTCGCTAAGATTTGTAAGAACGGCCCTGGt      <  1:117063/242‑1 (MQ=255)                                                                                                                                                                                                            aaCTCCGCTTTAGTACACAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGTTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATAC‑CACGGTAAGATTTGTAAGAACGGCCATGGtcg    >  1:465/1‑247 (MQ=255)                                                                                                                                                                                                            aaCTCCGCTTTAGCCCAAAGTTCGTCGATAGTACCACAGCCGGTCAGAACCATACAGGAGCGCCGTCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTAAGCGACCTTCGATACCTGCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGACATAACTGTCGGAGGAACCTTTGGACCTCGCGCCCAGGGAAAC‑ATTCACACGGTAAGAttttgaggataggccctggcaggt  >  2:159132/1‑230 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            AGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATG‑AAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAGCCGGTCAGACCCATACAGGAGCGCAGGCCGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATAC‑CACGGTAAGATTTGTAAGAACGGCCCTGGTAGAG  >  NC_000913/2632493‑2632945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑