Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,623,838	G→A	Q265Q (CAG→CAA)	ppk →	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,623,838	0	G	A	97.1%	93.0 / ‑4.0	34	Q265Q (CAG→CAA)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (12/21);  total (13/21)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

GCCAGAAGCGCCGCGTCGACGCAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTG‑ATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATC  >  NC_000913/2623601‑2624079                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  cccAGAAGCGCTCTGTCGACCCAAGCCGATGATTCTTCTGGATAACATTCTGTGTTACTGCCTTG‑ATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCa                                                                                                                                                                                                                                      <  2:163777/250‑1 (MQ=255)   aaaaaGCGCCTCTTCGACCTAAGCCGATGAGTCTGCTGGATAACATTTTGCGTTACTGCCGTG‑ATTATATTTTCAAAGGCTTATGTGATTATGACGCGCTGAATGCCTACTCATTTAAGATTACCCGCGATTCCGAATACGAGTTAGTGCATGAGATGGAAGCCGGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGAATCGTTTAACTGCTGAGCCTGTGCGGTTTTTTTATCAACGCGATATGCCCAAt                                                                                                                                                                                                                                    <  2:344027/248‑1 (MQ=255)         cgccgcGTCGACGCAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTG‑ATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCAAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTg                                                                                                                                                                                                                              >  2:89221/1‑251 (MQ=255)                   aCGCAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTG‑ATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAgtgt                                                                                                                                                                                                                    <  1:115810/251‑1 (MQ=255)                    cacaagactataatTCTTATGGATAACATTCGTCTTTACTGC‑TAGTATGATATTTCAAAAGGCTTAGTTGAGTATGACGAGTTGAAAGCTTATTCAATGAAGATGACCCGCGATGCCGAACACTATTTAGTTCAGGACATGGATTCCATTCTGACGGAGTTTATGTCTTCCAGTCTCAAGCAGCTTTTCATTGCTGTGCCGGTGTGTTTTGTTTATCAACTCCATATTCCCACTGCGCTGATTGAATTGtt                                                                                                                                                                                                                   <  2:64922/239‑1 (MQ=255)                               gatTCTTCTGGTGAACATTCGTCTATACTGCCTTT‑ATGATTTGTTCAAAGTCTTCGTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCTCGATGCCGAATACGATTTAGTGCATGAGATGTAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCATCTTTTAACTGCTGAGACGGTGCGTTTTGTTTAGCAACGCGATATGTCCAATGCGCTGGTTGAAGTGTTACTCGaaaaa                                                                                                                                                                                                         <  2:115245/250‑1 (MQ=255)                                                 tttgtgtttcttctttG‑ATTATATTTTCAATGGCTTCTTTGATTATGACCCGTTGAATGCCTATTCTATGAAGATGTCCCGAGATGCCGAGTATGATTTAGTGCAGGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCATTCTCAAGCAGTGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACTCGATATGCCCAATTCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTGCCCGCtcc                                                                                                                                                                                       <  2:265531/236‑3 (MQ=255)                                                      gTTACTGCCTTG‑ATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTcc                                                                                                                                                                                 <  1:97697/251‑1 (MQ=255)                                                       gtgacttccgttatgatATTTATAAAGACTTCTTTGCTTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATCCGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACGGCTGAGCCTGTTCGTTTTGTTTAGCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTcc                                                                                                                                                                                 <  2:261403/239‑1 (MQ=255)                                                                    tgatATTTTCAAAGGCTTCTTTGATTATGACGCGATGAATGCCTATTAAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAAGGCGCGGGTTGAAGTGTTACGCGAATAACTGACTATTTCCCGCTACGACTCACTCGTCCCcggcg                                                                                                                                                                    >  2:248091/1‑251 (MQ=255)                                                                              aaaGGCTTCTTTGATTATGGCGCTCTGAATGCCTATTCAATGAATAGGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGCAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCATCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACTACTCCATCGTCCCCGGCGGTCGTTATCa                                                                                                                                                          <  2:284873/251‑1 (MQ=255)                                                                                                 acgctcttattGCCTATGAAATGAAGATGACTCGCGATGCCGAATACTATTTAGTGTATTAGATGTAAGCCAGCCTGATTGAGTTGATTTCTTCCATTCTCAAGCAGCGTTTAACTTCTGCGCCGGTGCGTTTTGTTTATCAACGCGATATGACCAATGCGCTTGTTGAAGTGTTACGCGAAAAACTGTTTATTTCCCGCTACGACTCCATCGTCCCCGTCGGTCGTTAGCATAATTTTAAAgttttaatt                                                                                                                                       <  2:280504/241‑9 (MQ=255)                                                                                                        gAATGCCAATTCAATGAAGATGACCCGTGATGGCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGAGGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACGGCTGATCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTTACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTcc                                                                                                                                <  2:276649/251‑1 (MQ=255)                                                                                                            gCCTATTCAATGAAGATGACCCGCGATGCCGTATACTATTTAGTGCAGGATATGGAAGCCAGCTTGATGGAGTTGATGTTTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAACGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCa                                                                                                                             <  2:99350/250‑1 (MQ=255)                                                                                                             ccTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAAt                                                                                                                           <  1:176556/251‑1 (MQ=255)                                                                                                                aTTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCTGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTc                                                                                                                        <  1:113191/251‑1 (MQ=255)                                                                                                                aTTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTGACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGCCCCCGGCGGGCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTc                                                                                                                        >  2:134743/1‑251 (MQ=255)                                                                                                                         aGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGcc                                                                                                               <  1:111247/251‑1 (MQ=255)                                                                                                                               cccGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCAGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTg                                                                                                         >  1:230156/1‑251 (MQ=255)                                                                                                                                     aTGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAAc                                                                                                   >  1:231271/1‑251 (MQ=255)                                                                                                                                             tACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACt                                                                                           >  1:278350/1‑251 (MQ=255)                                                                                                                                                       tGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGGTATCATAATTTTAACGACTTTTTTAATTTCCCCAAAGTCGGCAAAGACAATCTGGTGAACAAACCAATGCCGCGtttt                                                                                 >  2:326335/1‑250 (MQ=255)                                                                                                                                                         cATGAGATGGAAGCCAGCCTGATGGAGTTGCTGTCTTCCAGTCTCACGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACg                                                                               >  1:104354/1‑251 (MQ=255)                                                                                                                                                                  gAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGTCTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTg                                                                      <  1:34630/251‑1 (MQ=255)                                                                                                                                                                                ggAGGTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGcc                                                         <  1:246572/250‑1 (MQ=255)                                                                                                                                                                                              ccAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGCTAAAGCCCAGTTCCGCAATGGt                                          >  1:95726/1‑251 (MQ=255)                                                                                                                                                                                                     cAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGa                                     <  1:269390/249‑1 (MQ=255)                                                                                                                                                                                                         cagcGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCAt                               >  1:178289/1‑251 (MQ=255)                                                                                                                                                                                                              tttAACTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGGGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGGTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTAGGCAAAGCCAATATGGTGAACAAACAACTCCCGCGTTTAAGCCATATTTGGTTTGATAAAGCCCAGTTACGACATGGTTTTGATGACATTcgcg                          >  2:96288/1‑251 (MQ=255)                                                                                                                                                                                                                 aaCTGCTGAGCCGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGaa                        <  1:128232/250‑1 (MQ=255)                                                                                                                                                                                                                            cGGTGCGTTTTGTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGCTGCCATTCGCGCACGCGATGTGTTg            >  1:332578/1‑251 (MQ=255)                                                                                                                                                                                                                                       gTTTATCAACGCGATATGCCTAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGATTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGAcgtt                                                                                                                                          <  2:213189/114‑4 (MQ=255)                                                                                                                                                                                                                                       gTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTTCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTGCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGTTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATc  <  1:72819/250‑1 (MQ=255)                                                                                                                                                                                                                                       gTTTATCAACGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACttt                                                                                                                                          >  1:213189/1‑114 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  GCCAGAAGCGCCGCGTCGACGCAAGCCGATGATTCTTCTGGATAACATTCTGCGTTACTGCCTTG‑ATGATATTTTCAAAGGCTTCTTTGATTATGACGCGCTGAATGCCTATTCAATGAAGATGACCCGCGATGCCGAATACGATTTAGTGCATGAGATGGAAGCCAGCCTGATGGAGTTGATGTCTTCCAGTCTCAAGCAGCGTTTAACTGCTGAGCCGGTGCGTTTTGTTTATCAGCGCGATATGCCCAATGCGCTGGTTGAAGTGTTACGCGAAAAACTGACTATTTCCCGCTACGACTCCATCGTCCCCGGCGGTCGTTATCATAATTTTAAAGACTTTATTAATTTCCCCAATGTCGGCAAAGCCAATCTGGTGAACAAACCACTGCCGCGTTTACGCCATATTTGGTTTGATAAAGCCCAGTTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATC  >  NC_000913/2623601‑2624079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑