Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,613,763 G→A E622K (GAA→AAA)  hyfR → DNA‑binding transcriptional activator HyfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,613,7630GA90.3% 77.9 / ‑1.6 31E622K (GAA→AAA) hyfRDNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (11/17);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 5.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCCAGAAAAGCGGCAATCGCTGAACGGATTGCCGCC  >  NC_000913/2613518‑2614005
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
ggAATGGCGCTATTGGCGCCATCCCGATCGAGGCATTACGCCAGTTGATGTGGTGGGATGGGCCGGGCGACGTGTGGGAGATGGAAAACGTGATTGAGTGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCGTCGCAGTTCAAGTGCGCAGTTACTGCCCCCGACGACGCCAGAGAATTACgaaaaagaa                                                                                                                                                                                                                                               <  2:255587/250‑1 (MQ=255)
       cgcaatgtacgctagcccGATCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTTCATCTAAAGGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCTCAGTTCAATGGCGCAGTTACGGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAgc                                                                                                                                                                                                                                        <  1:28845/236‑1 (MQ=255)
          aaTTGACGCCATCCCGACCGAGGAACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGCACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGAGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGCAGAAAAAGAACGTCAgcgc                                                                                                                                                                                                                                      >  2:224498/1‑250 (MQ=255)
           aTTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCCGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGACTGACGAAAAAGAACGTCAGCGCAt                                                                                                                                                                                                                                    >  1:249426/1‑251 (MQ=255)
             tGACGCAATCCCGACAGAGGCTCGACGCCAGTTGATGTCGTGGGATTGGCCGGGGAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCAtt                                                                                                                                                                                                                                   <  2:232733/250‑1 (MQ=255)
              gACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGAGTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTg                                                                                                                                                                                                                                  <  1:161085/250‑1 (MQ=255)
                     tctagaccgaGGCACTACGACAGTTGATGTCGTGGGATTGGTCGGGCACCGTGCGCGAGATGTAAAACGTGATAGAGCGGGCGGTACTGTTGACTCGTGGTAACAGGCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAAAGAAGCTTCAGCGCTTCGCAGTTACATGTCGCAGTTACTGCACCCGCCGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATAGTTCAgg                                                                                                                                                                                                                            >  2:134338/5‑249 (MQ=255)
                               ggcccTACGCCAGTTGATGTCGGGGGATTTGCCGTGCAACGTGCTCGAGCTGGAAAACGTGATTGGGCGGGCGGTACTGTTGCATTGTGGTAACAGTCGGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGaaa                                                                                                                                                                                                                <  2:91811/247‑1 (MQ=255)
                                     aCGCCAGTTGATGTCGTGGGATTGGCCGGGCAAAGTGCGCGAACTGGAAAACGTGCTTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACGAAGCCGTTTACTGCCGACTCTAAATGAAGATTCAGCGATTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGAAGCCAGAGAATGACGAGAATGAGCGGCAGCGCGTTGGTCAGGTATTGCGAGAACCCAATg                                                                                                                                                                                                          >  1:305713/1‑251 (MQ=255)
                                           gTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCAttg                                                                                                                                                                                                    <  1:224498/251‑1 (MQ=255)
                                            ttGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGAAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCAttgt                                                                                                                                                                                                   >  1:369962/1‑251 (MQ=255)
                                                 gTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCg                                                                                                                                                                                              >  1:9096/1‑251 (MQ=255)
                                                                 ggCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTCCATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGc                                                                                                                                                                              <  1:147942/251‑1 (MQ=255)
                                                                                                             gTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAAt                                                                                                                                    <  1:176910/249‑1 (MQ=255)
                                                                                                               tgacccgttgtaccatcatGAATTTACATCCAAATGTCCTGCAAAGCCGTTTACTGCCGGCGCTAAATGAAGATTCAGCGCTTCGCATCTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCa                                                                                                                                 <  2:9096/232‑1 (MQ=255)
                                                                                                                       ggTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTgg                                                                                                                         <  1:299058/250‑1 (MQ=255)
                                                                                                                         tAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGATGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTggggg                                                                                                                      >  1:134794/1‑250 (MQ=255)
                                                                                                                           aCAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGGGCAGCGACGCGATTCGGGATGAAGCGCACCACGCCGCTGTCACGAATGCAGCGGCTGGGGAtc                                                                                                                    >  2:339252/1‑251 (MQ=255)
                                                                                                                            cAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCAAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGAtct                                                                                                                   <  1:23977/251‑1 (MQ=255)
                                                                                                                                 tGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGtt                                                                                                              >  1:53185/1‑251 (MQ=255)
                                                                                                                                            cTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGAt                                                                                                                     >  2:114677/1‑233 (MQ=255)
                                                                                                                                            cTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGAt                                                                                                                     <  1:114677/233‑1 (MQ=255)
                                                                                                                                                          aaGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTg                                                                                     <  1:339252/251‑1 (MQ=255)
                                                                                                                                                                   aCTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCTAGGTGTTGTAATCTGCTTTTGCAGGAGTAt                                                                            <  1:293424/251‑1 (MQ=255)
                                                                                                                                                                                           cgctgttcggaggtCAATGGCGCAGTTCCTCCCTCCGACGACGCCAGAGAATGGCGAAAAAGAACGTCAGCGCATTGTTCAGTGATTGCGATAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAAGCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCg                                                    <  2:249426/238‑1 (MQ=255)
                                                                                                                                                                                             cgcTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGAc                                                  <  1:350237/251‑1 (MQ=255)
                                                                                                                                                                                             cgcTTCGCAGTTCAATGGCGCAGTTACTGCACCCGAAGACGCCAGAGAATGAAGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGAc                                                  <  1:85178/251‑1 (MQ=255)
                                                                                                                                                                                                 tCGCAGTTTAATGTCGCAGTTACTGCCACCGACGAAGCCAGAGAATGGCGAAAAAGAACGTAAGCGCATTGTTCAGGGAGATCAAGAAACCAATGGAATTGTTGCGGGGCCCCGTGGCGCAGCGACGCGAGTAGGTATGAAGCGCTCCACGCTGTTGTCACGAATTCAGCGGCTGGGGATCTCGGTGCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGAcgg                                                <  2:158672/249‑3 (MQ=255)
                                                                                                                                                                                                         cAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAg                                       <  1:98318/250‑1 (MQ=255)
                                                                                                                                                                                                                  cgTTACTGCACCAGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCATGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCCCCACGCTGCTGTCCCGAATGCAGCGGCTGGGGATATCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGGGGATGCATGAGACACAAACTTTCTTTCGACTTGCAGTTTATCGCCATaaa                              >  2:3120/2‑250 (MQ=255)
                                                                                                                                                                                                                                      ccAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTCATCTGCTTTTGCAGGAGGATGCATGAGAAACACACTCTCTTTCGTCTTGCCGTTGAGCCCCAGAAAAGCGGCAATAGCTGCACGGAt         >  2:295287/1‑251 (MQ=255)
                                                                                                                                                                                                                                             aTGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGAGCCACGTGGCGCAGCGACGCGGTTAGGGATGAAGCGCAACACGCTGCTGTCACGAATGCAGCGGCTGGGGATCGCGGTACGCGAGGAGTTGTACTCTGATTTTGAAGGAGTATGAATGGGAAACACACTCTCTATCTACTTGCAATTTATCGCACGAAACTCGGCAATCGCTGAACGGATTgcccac  >  2:155630/1‑248 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
TGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCCAGAAAAGCGGCAATCGCTGAACGGATTGCCGCC  >  NC_000913/2613518‑2614005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: