Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	774	T→C	T146T (ACT→ACC)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	774	0	T	C	88.0%	56.5 / 0.6	25	T146T (ACT→ACC)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (19/3);  total (20/5)
Fisher's exact test for biased strand distribution p-value = 9.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

GCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGG  >  NC_000913/530‑1018                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATc                                                                                                                                                                                                                                                <  1:193426/251‑1 (MQ=255)    aTGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGAt                                                                                                                                                                                                                                             <  1:330681/251‑1 (MQ=255)                  gtggacGAACCTTTGGCGGGACCTGCCGCCGCCCAGCCGGGGTTCCCGTTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATTGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAAc                                                                                                                                                                                                                                <  2:167112/245‑1 (MQ=255)                                   gggACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCAtt                                                                                                                                                                                                              <  1:37482/251‑1 (MQ=255)                                                      ccGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATAAACGCTGCGCTGATTGGCCGGGGCGAGAAAATGTCGATGGCAATTATGGCCGGCGAATTAGAAGCGCGAGGTCAAACCGTTACCGTTATCGATCCGGTCGAAAACCTGCTGGCAGTGGGGATTTACCTCGATTCTACGGTca                                                                                                                                                                                            >  1:3628/1‑249 (MQ=255)                                                                  ttggagcAATTGAAAACTTTCGGCGAGCAGGAATTAGCCCAAATAAAACATGTCATGCATGGCATTAGTTTGTGGAGGCAGTGCCCGAATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCAATTATAGCCGGCAACTTAGAAGCGAGCGGTAACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGAAGTGGGGCATTAACGCGAATCTACCGTCGAAATTGCTgtttc                                                                                                                                                                               >  2:321434/6‑247 (MQ=255)                                                                                             cAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATAGTGCTGAGTCCAcc                                                                                                                                                                           <  1:306214/228‑1 (MQ=255)                                                                                             cAGGAATTTGCCCAAATAAAACATGTACTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAAAGTTTCCGTTATAGGTCCGGTCGAAAAACTGCTGGCAGTGGGGCCTTACCTCGAATCTACATTCGATATTGTTGCGTCCAccagatcggcagagcgtattgtagg                                                                                                                                                    >  2:306214/1‑228 (MQ=255)                                                                                              aGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCAtt                                                                                                                                                   >  1:320239/1‑251 (MQ=255)                                                                                                 aaTTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCg                                                                                                                                                >  1:295145/1‑251 (MQ=255)                                                                                                 aaTTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCg                                                                                                                                                >  1:44684/1‑251 (MQ=255)                                                                                                 aaTTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGATGCGCTGATTTGACGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTCCCGTTATCGATCCGGTCGAAAAACTGCTGGCCGTGGGGCATTACCTCGACTCTACCGTCGATATTGATGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCg                                                                                                                                                >  1:252743/1‑251 (MQ=255)                                                                                                                       ccTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGCAACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGCGTCCACCCGCCGCATTCCGGCAAGTCGCATCCCGGCTGGTCACATGGGGCTGATgg                                                                                                                          >  2:93164/1‑251 (MQ=255)                                                                                                                                                         gATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGGATTGCGGCAAGGCGCATTCCGGCTGATCACATGGTACTGATGGCAGGTTTCCCCGCCGGTAATGAAAAAGGCGac                                                                                          >  2:276755/1‑248 (MQ=255)                                                                                                                                                            aGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACtggg                                                                                     >  1:117551/1‑250 (MQ=255)                                                                                                                                                                                     gTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGac                                                            >  1:77444/1‑251 (MQ=255)                                                                                                                                                                                         cGAGAAAATGACGATCGCCATTATGGCAGGCGTATTAGAAGCGCGCGGTAACAACGTTACCGTTATCGATACGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGCTATTGCTGCGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCAAATGGTGCTGATGGCAGGTTTACCCTCCGGTAATGAAAAAGGCACACTGGTGGTGATTGGGCGGAAAGGTTCCGactacc                                                        >  2:44618/1‑250 (MQ=255)                                                                                                                                                                                                aTGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTActccgcggc                                                 >  1:96321/1‑245 (MQ=255)                                                                                                                                                                                                    cGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACACCGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGAAATGAAAAAGGCGAACTGGTGGTACTTGGGCGCAACGGTTCCGACTCCTCCGCGGCGGTg                                             >  2:150776/1‑251 (MQ=255)                                                                                                                                                                                                     gATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTACTGAAAAAGGCGAACTGGGGGAACTTGGACGAAACGGGTCCGaatactacgcggcggggg                                            >  2:208118/1‑239 (MQ=255)                                                                                                                                                                                                         gCCATTATGGCCGGCGTATTAGAAGCGCGCGGGCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTACGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGCCTACTCCGCGGCGGAgctggg                                        >  1:318659/1‑250 (MQ=255)                                                                                                                                                                                                          ccATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAACAACTGCTGGCAGAGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGATGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTgctggct                                       >  1:44685/1‑251 (MQ=255)                                                                                                                                                                                                                  gCCGGCGTATTAGAAGAGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTggtt                               >  1:329575/1‑248 (MQ=255)                                                                                                                                                                                                                          aTTAGAAGCGAGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAACATGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGTGTCCACCCGCCGTATTGAGGAAAGTCGCATTCCGGCTGATCCCATGGTGCTGATGGCAGGTTTAACCGCCGGTAATGAAAAAGGCGTACTGGTGGTCCTGGGACGCAACGGTTCAGACTACGCCGCGGCGGTGAGGGCTACCTGTTTACGCGCCg                       >  2:226888/1‑251 (MQ=255)                                                                                                                                                                                                                                               cGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCAGACTACTCCGCGGCGGTGCTGGCTGCCTGATTGAGCGGCGATTTTTGCGAGATTTggacgg  >  2:272852/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGG  >  NC_000913/530‑1018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑