Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,388,753	G→A	G40D (GGT→GAT)	yfbO →	uncharacterized protein YfbO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,388,753	0	G	A	93.8%	42.7 / NA	16	G40D (GGT→GAT)	yfbO	uncharacterized protein YfbO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (5/10);  minor base C (1/0);  total (6/10)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCTGCTTAACATTCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTT  >  NC_000913/2388508‑2388945                                                                                                                                                                                                                                                      |                                                                                                                                                                                                 tCCTGCTTAACATTCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCa                                                                                                                                                                                              <  1:275304/250‑1 (MQ=255)               cATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAAtgtg                                                                                                                                                                               <  1:259123/251‑1 (MQ=255)                          gCTCTTGAAAATAATAATACGTATGGAATTGTCACTATTTATCAGCTAATTAATTCAAACAATGTCAGTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTAACCAACTTGTTAATATCAATGGTGATGTTAATATTCCTAGTACGCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATCATATCCATGCTTCACTATGTTGCAATCTGATTCTAGaaa                                                                                                                                                                     >  2:291552/1‑249 (MQ=255)                                            aCGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGctatcta                                                                                                                                                  <  1:250655/251‑1 (MQ=255)                                                        gTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTc                                                                                                                                       <  1:161678/250‑1 (MQ=255)                                                                      gCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGc                                                                                                                        >  1:138641/1‑251 (MQ=255)                                                                             aaTTCAAACAATGTCATTCGTTTGATGAATATATTATTTAAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCAATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATACATGATTCAATATGTTGCAATGTGATTACAGAACAATCTCCGCAAGCTATATATCACCATTTTCTGAAAATACGTGAGCGTAACa                                                                                                                  >  1:66538/1‑250 (MQ=255)                                                                              aTTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAAt                                                                                                                <  1:148631/251‑1 (MQ=255)                                                                                                                             atTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCACTGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGACAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACAATGTTCGATGACCCAGACTg                                                                 >  2:276842/1‑251 (MQ=255)                                                                                                                                atgtcacCATTAGAACGTCTTCCCCAAATTGTTAATATCAAAGGTGATGTTAATTATCCTGATACTCCTCGTCCATTTTTATCTATTTAGTAATTTTTTATGGACAATAATATCCATTATTCAATATGTTGCAATGAGATTCCAGAACAATCTCCGCAAGCTAGCTATGACCATTTTCTGAAAATACGTGAGCGTAACAATGTGAGTGATGTTTTGGTAGAAATTGCGCTGTTCTATGACCCAGACTgga                                                               <  2:73743/246‑2 (MQ=255)                                                                                                                                aTGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGaa                                                                                                                                                                      <  1:165359/147‑1 (MQ=255)                                                                                                                                aTGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGcc                                                              <  1:372860/251‑1 (MQ=255)                                                                                                                                aTGACACATTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCCTGATTCAATATGTTGCAATGTGATTCCAGaa                                                                                                                                                                      >  2:165359/1‑147 (MQ=255)                                                                                                                                 tGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGGGATGTTAATAATCCTGATACTCCTCGTCCATTATTATCTCTTGAGGAATTTTTTATTGCCAATAATATCCATGATTAAATATGTTGCAATGTGATTCCAGAACAATCTCCGAAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTACGATGACACAGACTGGccc                                                             >  1:356728/1‑251 (MQ=255)                                                                                                                                   acacCATTAGAACGCATTACCCAACTTGTTTATATCAATGGTGATGTTAATAATCCGGATACTCCTCGTCCATTGTTAGCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCtt                                                           <  2:138641/251‑1 (MQ=255)                                                                                                                                                                                            gATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGATTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGtt  <  1:294228/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                 TCCTGCTTAACATTCATGTTATGATGCTCTTGAAACTAATAATACGTATGGAATTGTCACTATTTATCTGCTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTT  >  NC_000913/2388508‑2388945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑