Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,348,366 C→A T328K (ACG→AAG)  nrdB → ribonucleoside‑diphosphate reductase 1 subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,348,3660CA90.0% 68.1 / ‑0.4 30T328K (ACG→AAG) nrdBribonucleoside‑diphosphate reductase 1 subunit beta
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (12/15);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGACGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGG  >  NC_000913/2348121‑2348607
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
cctgCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGTCGGAAATTGCCGAAGAGTGTAAGCAGGATTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCt                                                                                                                                                                                                                                                <  2:294721/248‑1 (MQ=255)
        tGCTGAATCTGCGGCTCAGGGGCGCGGACGATCATGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTAGGACCTGTTTGGTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTGCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAAccc                                                                                                                                                                                                                                         <  2:274031/250‑1 (MQ=255)
                   ctgcGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGACGCGCTCCAACCCGATCCCGTGGAt                                                                                                                                                                                                                             >  2:289849/1‑251 (MQ=255)
                                    cGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGgtgt                                                                                                                                                                                                            <  1:289849/251‑1 (MQ=255)
                                       tCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTg                                                                                                                                                                                                         >  1:174890/1‑251 (MQ=255)
                                           gagaTGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATa                                                                                                                                                                                                      <  1:268883/250‑1 (MQ=255)
                                                ggCGGAAATTGCCGAAGATTGTAAGCAGGAGTGCTATGACCTGTTTGTGCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTGCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGc                                                                                                                                                                                               <  1:179550/251‑1 (MQ=255)
                                                      aaTTGCCGAAGAGTGTAAGCAGGATTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTGCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCTCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTg                                                                                                                                                                                         <  1:223274/251‑1 (MQ=255)
                                                      aaTTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGCTTGGTATGAATAACGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAA‑GTGCAgggtg                                                                                                                                                                                         >  2:29497/1‑248 (MQ=255)
                                                       aTTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGc                                                                                                                                                                                        >  1:364212/1‑251 (MQ=255)
                                                                         gtggaggtcTATGATCTTTTTGTTAAGGCAGCTCAACTGATGCAAGACTTTGTGGTTTCTCTGTTCCGCGACGTGTT‑ATTGATGGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATGCATCTCCAAGATCCGTATGCCGGCAGTCTGTCTGGATCTGCCGTTCCTTAAGCGCTCCATCCCGATCCCGTGTATCAACACTTTGCTGGTGTCTGATAAC‑GTTCAGGTTGCTCCACAggaagtggaag                                                                                                                                                                       <  2:240788/242‑1 (MQ=255)
                                                                          aGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGACGTGGAAGTc                                                                                                                                                                     >  1:155242/1‑251 (MQ=255)
                                                                                    tGACCTATATGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGAAATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCACGTGGATCAACACTTGGCTGGTGTCTGATACC‑GTGCAGGTTGCTCCGCAGGAAGTGGGAGTCCGTACTTATc                                                                                                                                                           >  2:144814/1‑251 (MQ=255)
                                                                                            ttgttCAGGCATCGCAACAGGAAAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTTTCTGCCAGTACGTTGAATACATCACCAATACCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGACGCGCTCCAACCCGATCCCGTGGAGCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCgg                                                                                                                                                    <  2:145773/250‑1 (MQ=255)
                                                                                               ttCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGACAGTACGTTGAATACATCACAAATATCCGTTTGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAATGGAAGTCAGTTCTTATCTGGTCGGGc                                                                                                                                                  >  1:248748/1‑249 (MQ=255)
                                                                                               ttCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGATCTGCATAAAGACCTTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCAGTTCCAGAAGCACTCCAACCCGATCCAGTGGATCAACACTTGGCTGGTGGCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGCAGTCAGTTCTTATCttgcggggca                                                                                                                                                 >  2:4003/1‑241 (MQ=255)
                                                                                                tCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAg                                                                                                                                                <  1:276652/250‑1 (MQ=255)
                                                                                                    gCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGATCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGa                                                                                                                                           <  1:162919/251‑1 (MQ=255)
                                                                                                            aCAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGa                                                                                                                                     >  1:257339/1‑249 (MQ=255)
                                                                                                                                                cG‑GTGCGATGATTTGTCTGAATAATGACATACTCTGCCAGTACGTTTAATACAGCTCCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCTTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAAATCTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGTGTAACTGCCAGCTATGa                                                                                               <  2:173303/250‑1 (MQ=255)
                                                                                                                                                                 cTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCATCTCTGATGGCCCGCGTTACCCTg                                                                              >  1:242559/1‑251 (MQ=255)
                                                                                                                                                                    aaTAAAAACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTCTGCAGACAGTCGGTTTGGATCTGACGTTCCAGAAGCGCTACAACCAGATCACCTGGATCAACACTATGCTGGTGTCTGATAACAGT‑AAGGGTTCTCCGCAGGACGGTGAAGACAGGTATTATCTGGTCGGGCAGATTTCCTCGGACAG‑GGACACCGACGATTTGAGTAAATTCCAGCTATGATGGCCCGGGTTAcccatcgc                                                                           >  2:291868/1‑246 (MQ=255)
                                                                                                                                                                                 tctGCCAGAACGTTGAATACATCACCAATATCCGTATGAAGTCAGTCGGGTTGGATTTGCCGTTCCAGAAGCGATCCATCCCGATCCAGTGGATCAACACTTGGCTGGTGTCTGATATA‑GAGCAGGTTACTCCGCAGGAAGTGGAAGTCAGTTCTTATCTAGTCGGGCAGATTGACTCGGA‑AGTGGAACCCGACGCTTTGAGTACTTTCCAGCTCTGATGGCCCGCGTTACCCTGCACATAACTGGcacc                                                               >  2:224970/1‑249 (MQ=255)
                                                                                                                                                                                           cGTTGAATACATCACCAATGCCCGTAGGCAGGCAGTCGGTTTGGTGCTGCCGTGCCAGAAGCGCTCCAACCCGATCCCGTGGAGCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCAGCGCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCtgt                                                     <  2:174890/250‑1 (MQ=255)
                                                                                                                                                                                            gTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGc                                                   <  1:29497/251‑1 (MQ=255)
                                                                                                                                                                                               gAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGACGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGcc                                                  >  1:118015/1‑249 (MQ=255)
                                                                                                                                                                                                     aTCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGaa                                          <  1:329178/251‑1 (MQ=255)
                                                                                                                                                                                                                ccGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACAcccttccct                               <  1:51353/251‑1 (MQ=255)
                                                                                                                                                                                                                ccGTACGCAGGCCGGCGGTTTGGATCTGCCGTTCCTGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGATGTGGAAGTCAGTTCTTATCTGGTCGTGCCGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACAGGCACGCTACTGCTGTGCAATGATGAACAcccttccc                                <  1:278923/250‑1 (MQ=255)
                                                                                                                                                                                                                                             cGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTgg  >  1:105011/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
CCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACG‑GTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGACGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAAC‑GTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGA‑AGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGG  >  NC_000913/2348121‑2348607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑