Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,760	T→C	A808A (GCT→GCC)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,760	0	T	C	96.3%	70.3 / NA	27	A808A (GCT→GCC)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (15/11);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

ACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑TTGAGGCGGCAG‑AGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTT  >  NC_000913/2513‑2996                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               gcgttcgctTTGCCGCGCGCGGGGCGAAGGCCCGTGATGAAGGAAAATTTTTGCGCTTTGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCTGTTGTCTTTGCCGAt                                                                                                                                                                                                                                             <  2:369989/244‑1 (MQ=255)                cgcgcgTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCctc                                                                                                                                                                                                                              <  1:223112/251‑1 (MQ=255)                 gctcgTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCTTGCCGCGTGAAGATTGCCGAAGTTGATTGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCctct                                                                                                                                                                                                                             <  1:359254/248‑1 (MQ=255)                     gTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATg                                                                                                                                                                                                                         >  1:314631/1‑251 (MQ=255)                         cGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGa                                                                                                                                                                                                                       >  1:174256/1‑249 (MQ=255)                                           gAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGt                                                                                                                                                                                                   >  1:328350/1‑251 (MQ=255)                                                    tGCGCTATGTTGGCAATATTGATGAAGATGGTGACTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGttt                                                                                                                                                                                           >  1:321635/1‑250 (MQ=255)                                                               ggCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCtt                                                                                                                                                                               >  1:320943/1‑251 (MQ=255)                                                                          tgaAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAat                                                                                                                                                                    >  1:186355/1‑251 (MQ=255)                                                                            aagatggtaccTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGAGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCAGTTGGTGCTGCGCGGGTAAGGTGCGGGCAATGCCGTAACAACTGCCGGTGTCTTTCCCGTGCTGCACCGTACCCTCTCATGGAAGATAGGAGTCTGCCATGTTTACAGTTTATGCCCCGGCATCCAgaaccacac                                                                                                                                                                   >  2:290552/11‑242 (MQ=255)                                                                                                aataTTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGGGACAAATGGCGCAAAAGCCCGGGACGTTAAGAGCCACTATTATCAGCAGATACAGTTTGTGCTGAGCGGATATGGTGCGGGCAATGCAGATAAGGCCGCCGGTATATTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGGGTAGGACATGGGTAAAGTTTATGCCCCGGCTTCAAGTGCAAAAATGTGAGTCGGGTTTggggtt                                                                                                                                               >  2:329010/4‑245 (MQ=255)                                                                                                aaGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCAATATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGAGTCGGGTTTGATGTGc                                                                                                                                              >  1:349342/1‑251 (MQ=255)                                                                                                                         aTCCGCTGTTCAAAGGGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGCTATGGTGCGGGCAATGACGGTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGCCTGACATGGTTAACGTTTATGCCCCGGCTTCCAGGGCCAATATGAGCGTCGGGTTTGCTGTACGCGGGGCGGAGGTGAAACCTGTTg                                                                                                                      >  2:13191/1‑250 (MQ=255)                                                                                                                                          aaaaaTGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGgag                                                                                                    <  1:114044/251‑1 (MQ=255)                                                                                                                                              aTGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGt                                                                                                >  1:320071/1‑251 (MQ=255)                                                                                                                                                        cGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑tt                                                                                     >  1:364497/1‑251 (MQ=255)                                                                                                                                                              ggccgtttaacgccccTATTCTCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAAGGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGTAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGGGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTTACGG‑TTGAggcg                                                                               <  2:320943/236‑1 (MQ=255)                                                                                                                                                                      ataGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCGATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCTGAGATGTAGTCACGG‑TTGAGGCGGCAg‑aga                                                                       <  2:353478/250‑1 (MQ=255)                                                                                                                                                                               attatCATCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑TTGAGGCGGCAG‑AGACATTCAGtc                                                              <  1:304960/250‑1 (MQ=255)                                                                                                                                                                               attagCAGCCGCTGCCGTTTGTGCTGCGCGGATATGGTGCGGGCAATGACGTGACAGCTGCCGGTGGCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑TTGAGGCGGCAG‑AGACATTCAGtc                                                              <  2:17756/250‑1 (MQ=255)                                                                                                                                                                                                 tGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑TTGAGGAGGCAG‑AGACATTCAGTCTCAACAACCTCGGACGCtt                                           >  1:329017/1‑251 (MQ=255)                                                                                                                                                                                                      cTGCGCGGGTATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGATAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCATTTGTTTGAGCATTGCCCGTAGATGTAGTCACGGAGTG‑GGCGGCAGTAGAC‑TTCAGTTTCAACAAccaaggatactttgccg                                      >  2:308905/1‑236 (MQ=255)                                                                                                                                                                                                             gtaatggggctggCAGTGACGTTGCATATGCAGGTTTCTTTGCCGATCTGCTACTTTCCTTCTCAGGGAAGTTTAGAGTCTGATATGTGTAATGTATATGCCCCGGCTTCCCGTGTCAATATGAGCGTCGGGTTTTATGGGCTCGGTGCGGCGGTGACACCTGTTGATGGTGCATTGCTCTGAGATGTAGTGTCTT‑TTGAGTTGGCAG‑AGACATTCAGTCTCAACATCCGCGCACGCGTTGCCTATAAGCt                               <  2:323574/240‑1 (MQ=255)                                                                                                                                                                                                                             ggtcgttcccgcgtccGGTGTCGTTGCCGATCTGCGACGTACCCTCTCATGGAAGTTAGGAGGCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑TTGAGGCGGCAG‑AGACATTCAGTCTCAACAACCTCGGTCGCTTTGCCGATAAGCTGCCGTCAGAACCACgg               <  2:261322/237‑1 (MQ=255)                                                                                                                                                                                                                              gACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑TTGAGGCGGCAG‑AGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACggg              >  1:11774/1‑251 (MQ=255)                                                                                                                                                                                                                                       gttGCCGTTGTCTTTGCCGAGCTGCTACGTTCCCTCTCATGTAAGTTATGAGTCTGTCATGGTTAAAGTTTATGCCCCGGCTTCCAGTTCCAAGATGAGCTGCGGGTTTGATGGTCTCGGGGCGGCGGGGACGCCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑TTGAGGCGGAAG‑AGACATTCAGTCTCAACAACCTCGGACGCTTTGCAGATAAGCTGCCGTCAGAACCACGGGAAAAAATca     <  2:206428/249‑2 (MQ=255)                                                                                                                                                                                                                                          gCCGGTTTCTTTGCTGATCTGTTACGTACCCTCGCATGGAAGTGAGGAGTCTGACATGGTTAAATTTTATGCCCCGGCTTCCAGGGCCAATATGAGCGTCGGTTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑TTGAGGTGGCAG‑AGACATTCAGTCTCAACAACATCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGttt  <  2:16735/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTGACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGG‑TTGAGGCGGCAG‑AGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTT  >  NC_000913/2513‑2996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑