Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	100.0%	73.6 / NA	27	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/17);  total (10/17)

CCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCA  >  NC_000913/1358628‑1359093                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            ccTTCTCCTTTATGCAGTCCCTGCACTACCAGGAAGATAAACACCCCCATGATGGAGATTTGCACCAGTACAAACAAGGTATTGAAGTTAGCGACCTGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGAc                                                                                                                                                                                                                            <  2:138078/249‑1 (MQ=255)           tATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑GAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCAt                                                                                                                                                                                                                 >  1:360327/1‑250 (MQ=255)           tATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGAt                                                                                                                                                                                                                               <  1:212943/236‑1 (MQ=255)            aTGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATa                                                                                                                                                                                                                <  1:325877/250‑1 (MQ=255)                 gTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCg                                                                                                                                                                                                          <  1:127995/251‑1 (MQ=255)                       gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCAGCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTg                                                                                                                                                                                                     <  2:360327/250‑1 (MQ=255)                                         acacCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTgc                                                                                                                                                                                  <  1:341824/251‑1 (MQ=255)                                            ccaccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTAC‑CACCCACACCCACGGCGGCACCTCCGGG‑GAGAGGGCGGGGAGATAGATTTTTGCCAACAAGACGTTAATCATCGGCAAAAAAAAACAATCCAGCAACGCTGACCAGCCGACCATAAATCCGAAGTGCGGGGTAATCGACTTTTGcgcg                                                                                                                                                                               >  1:319021/1‑251 (MQ=255)                                                        acgcttttccgagcaCAAACATGGTAGTGAAGTTAGCGACCAGGTTGACGCTCGTCAGATGCGCGGCGGGTAAAAAGGCGACGAAGGTTAA‑CACACACACCCACGGCGGCACTGCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAAGCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGACGACCATAAATCCGACGTGCGGGTTAATCGACGTTTGCGCGTAGGTATAGGcc                                                                                                                                                                   <  2:305178/237‑1 (MQ=255)                                                             tGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCCTAAATCCGACGTGCGGGGTAATCGACTTTTGCGCGTAGGTATAGGCCGCA‑cc                                                                                                                                                             >  1:53683/1‑251 (MQ=255)                                                                       cAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCCCACCCCCGGCGGCCCTTCCGGG‑GAGAGGGCGGGGAGATAGATTTTTGCCCACAAGACGTTAATCATCCGCACAAAAAGACCATCCAGCAGCGCTGACCAGCCCACCATACATCCGACGTGCGGGTTAATCGACTTTTTCCCGTTGGGATAGGccagatcggtagcgccc                                                                                                                                                    >  1:305178/1‑236 (MQ=255)                                                                        aaaCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAAGCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGaa                                                                                                                                                  <  1:348536/251‑1 (MQ=255)                                                                                              aCCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTa                                                                                                                            <  1:236964/251‑1 (MQ=255)                                                                                                               cAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACa                                                                                                           <  1:87660/251‑1 (MQ=255)                                                                                                                agatgctcgtgtgTTAAAATGCCGACGAGGGTGACTCCCCCA‑ATCCACGGCGGCACGTCCGGG‑AAGAGCGCGGAGAGATTGATTTTCGCCAACAAGACGTTAAACATCGGCCAAAAGAGACAACCCAGCAGCGATGACCAGCATACCATAACGCCGACGTGCGGGTTAATCGCCTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCACCGGAAACTGGTGCACCAGTTTGCCGTATCTGATAGCTGTAAACAg                                                                                                          <  2:46992/239‑1 (MQ=255)                                                                                                                                          gaAGGTTCC‑CACCCACACCCCCGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGAAAAAAGAGACAACCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGTTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAgg                                                                                <  1:274193/251‑1 (MQ=255)                                                                                                                                                            cccctcggggcACTTTCGGGGAAGAGGGGGAAGAAAAAGATTTTCGCCACCAAGACTATAATCACCGGAAAAAAGAAACAATCCAGCAGTGTTGACCAGCCGACCATAAACCCGACGTGCGGGTTAATCTGTTTTGGCGCGTGGGTATAG‑CCGAAACCGGCCCCCGGAAACTGGCGAACCTTTTTGCCGTAGCGGATAGCGGTAAACAGAACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGAcc                                                                <  2:367042/243‑1 (MQ=255)                                                                                                                                                              cccggcggCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACTTTAATCATCGGAAAAAAGAGACAACCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGATTTTGGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCCCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTc                                                             <  1:172296/249‑1 (MQ=255)                                                                                                                                                                 ggcggcACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCt                                                          >  1:339436/1‑251 (MQ=255)                                                                                                                                                                    ggcACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAAt                                                       <  1:290979/251‑1 (MQ=255)                                                                                                                                                                           ccGGG‑AAGAGGGCGAAGAGATAGATTTGCGCAAACAAGACGTTAATCAACGGCAAAAAGAGACAAGCCAGCAGCGAGGACCAGCCGACCATAACTCCGCCGGGCGGGTTAAGCGACGTTGGCGCGTAGGAATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGAAAACACCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGgag                                                <  1:94854/251‑1 (MQ=255)                                                                                                                                                                                 aaGAGGGCGGAGAAATAGATTTTCCCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACTTGCGGGTTAATCGATTTTTGCGGGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTGGCCGTAGCTGATAGCGTTAAACAGCACACCCGCCAGCGCCAGCGAATAGGTCGCCGGAACGTGACCGTCGCTAATGCCGGAGACAAt                                           <  1:152004/251‑1 (MQ=255)                                                                                                                                                                                          gagagaTAGATTTTCGCCACCAAGACGTTAATCATCGGCAAACAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGCGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCCGTTTGCAGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCACATCGGACGCCGGAACGTGACCGGCGCTAATGCCgcagtaaaaacccaaagg                                  >  2:156180/1‑234 (MQ=255)                                                                                                                                                                                               atagatTTTCGCCAACAAGACGTTAGTCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGACGCTAATGCCGGAGACAATACCAAAAGTATCa                              >  1:7565/1‑250 (MQ=255)                                                                                                                                                                                                                       aaTCATCGGCAAAAAGAGACAATACAGCAGCGATGACCCGCCGACCATAACTCCGAAGTGCGGGTTAATCGACTTTTGCGCGTACGGATATGCCGAC‑ACGGCCTCCGGAACCTGGCGAACCAGGTTGCCGTAGCTGCTCGCGGTCAGCAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGCACGAGACCGTCGCTAGTGCCAGAGACAATCCCACAAGAATCAAATACGGTCATCGGCGTgagcgagg     >  2:38961/1‑246 (MQ=255)                                                                                                                                                                                                                         tcatcGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGcc   >  1:93736/1‑251 (MQ=255)                                                                                                                                                                                                                          catcGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAACGTATCAAATACGGTCATCGGCGTGAGATAGGccc  >  2:74171/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            CCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAC‑CACCCACACCCACGGCGGCACTTCCGGG‑AAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAA‑CCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCA  >  NC_000913/1358628‑1359093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑