Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	96.5%	79.0 / ‑4.4	29	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (14/14);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

CCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCG‑GGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCC  >  NC_000913/1337150‑1337616                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 cccAAGTGTGTTGATGGCCGCATGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAGGCGGCATCCATGGTTCAAAGCCCCGCTGGCACCGGGTGCGAAAGTCGGTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGATCTGGGGTTTAACCTTGTGGGATACGGTTGTACCCCCTGTATTGGTAACTCTGGTCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGAtt                                                                                                                                                                                                                           <  2:307270/251‑1 (MQ=255)       tgtgCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCg                                                                                                                                                                                                                     >  1:230970/1‑251 (MQ=255)                 gCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTcggtgcggg                                                                                                                                                                                                           >  1:220081/1‑250 (MQ=255)                 gCCGCAGGCGTGCCGGCGAAAACAGCCGTAACTCTGTGCCTCAAGCGGTAACCATGTGTCAATGCTTCGCTGGCGCCGTGTCCGAAAGTCGTGTCTGATTATCTGGCAAAAGCGGAACTGACACCGTATCTCGACGATCGGGGGTTTAACCTTGTGGGATACGTTTGTACCACCTGTAGTGGTAACTCTTGGCCGCTTCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTcgttgcggt                                                                                                                                                                                                           <  1:173498/251‑1 (MQ=255)                  ccGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGGCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTcggtgcggtg                                                                                                                                                                                                          <  2:142689/251‑1 (MQ=255)                                              aaCTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGcc                                                                                                                                                                              <  1:89581/251‑1 (MQ=255)                                               aCTCTGGTCCTTAAGCGGCAACCATGGGTCAAATCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACCCCGTATCTCGACGAACTGGTGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCg                                                                                                                                                                             <  2:331737/251‑1 (MQ=255)                                                    gggCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatcc                                                                                                                                                                        <  1:276861/251‑1 (MQ=255)                                                       ccTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGTTTCGAAAGTCGTTTCTGATTAACTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAAGCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatccatc                                                                                                                                                                     <  1:179937/251‑1 (MQ=255)                                                               ggCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGtt                                                                                                                                                             >  1:314975/1‑251 (MQ=255)                                                                   aCCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaaaa                                                                                                                                                         <  1:339327/251‑1 (MQ=255)                                                                                    cgcttacacagggatcaaaaGTCTTTTGTGATTATCTGGCAAAAGCGAAACTGACACCGTCTCTCGGCGAATTGTGGTGTCACCTTGAGGGAAACGGTTGGACCACCTGTATGGGTAACTGCGCGCCGCTGCCCGATCCTATCGAAACGGCAAACAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAGCCGTAACTTTGAAGGCCATAGCCATCCGCTCGGTAAAACTAACTGGCTGGccgc                                                                                                                                         <  2:90645/233‑3 (MQ=255)                                                                                                gTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtg                                                                                                                            >  1:257157/1‑251 (MQ=255)                                                                                                    gAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTg                                                                                                                        <  1:184326/251‑1 (MQ=255)                                                                                                          ggTTTCTGCTTATCTGGGAAAAGCGAAACTGACACAGTATCTCGACGAACGGGGGTTTCACCTTGTGGGATACGGATGTACCACCTGTATTGGTACCTCTGGGCCGCTGCCCGATCCTATCGAAACGGCCATCAAACAAGC‑CGATTTAACCGTCGGTGCGGTGATGGCCGGCAACAGTATCTTTGAAGGCAGTATCCATCCGCTGGTTAAGCATAACTGGCTGGCCTCGCCGCCGATGGTGGTTGCCTAtc                                                                                                                  >  2:113167/2‑250 (MQ=255)                                                                                                                tGATTATCTGGCCAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTgg                                                                                                            >  1:74699/1‑251 (MQ=255)                                                                                                                  atgaTCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGTTAACTCCGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGGCGGTGCGGTGCTGTCCGTCAACCGTACCTTTGAAGGCCGTATCCACCCGCTGGTTAAAACTATCTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCg                                                                                                          <  1:1448/248‑1 (MQ=255)                                                                                                                         tgaaaaaGAGAAACTGACACCGGATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTAGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACAGCAATCAAAAAAGG‑CGAATTAACCGACGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTGGCCTGTACGCTGGCg‑ggga                                                                                                     >  2:284408/4‑246 (MQ=255)                                                                                                                                    acctgccacCGAATCTAGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTACCAGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAGAACTAACTAGCTGGCCTCGCCGCCGCTGGTGTGAGCCTCTGCCCTGGCGAGGAAatacaattccgc                                                                                         >  2:193022/7‑241 (MQ=255)                                                                                                                                      cTGACAACGTATCTCGCCGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGCTCCTATAGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCTGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTAGCCGCCGCTGGGGGTTTCCTATGCACTGGCG‑GGGAatgtagatataaacc                                                                                      >  2:207082/1‑237 (MQ=255)                                                                                                                                           acCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCG‑GGAAATATGAATATCAACCTGGCtt                                                                                <  1:207082/251‑1 (MQ=255)                                                                                                                                             cGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGT‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCG‑GGAAATATGAATATCAACCCGGCTTCt                                                                              >  1:304796/1‑251 (MQ=255)                                                                                                                                             cGTATATCGCCGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTAGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCAATCCGCTGGTTAAAACTAACTGGCTGGACTCGCCGCCGCTGGAGGATGCCTATGCTCTGGCG‑GCAAatagtcctattcaaccggcttct                                                                              >  2:346118/1‑231 (MQ=255)                                                                                                                                                          aCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCG‑GGAAATATGAATATCAAACTGGCTTCTGAGCCTATCggg                                                                  >  1:243727/1‑249 (MQ=255)                                                                                                                                                                gTTTAACCTTGGGGGATACGGTGGTACCACTGGTATGGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCAGCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCG‑GGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATc                                                           <  1:336170/251‑1 (MQ=255)                                                                                                                                                                       cTTGTGGGATACGGTTGAACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAACCGGCCATCAAAAAAGG‑CGCTTTACCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTCACTGGCTGGCCTCGCCGCCGCTGGTGTTTGCCTATGCGCATGCG‑GGAAATATGGATTACAACCCGGCGTTTGAGCCTAACGGCCATggacgcatagg                                                    >  2:370690/1‑241 (MQ=255)                                                                                                                                                                                           caccTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCG‑GGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGCTCCGGTTTTTCTGaaa                                 >  1:322141/1‑250 (MQ=255)                                                                                                                                                                                                              gggCCGCTGCCGGATCCTACCTAACCGGCAA‑CAAAAAAAGGCGATTTAACCGTCGGGGCGGTGCGGTCCGGCATCCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCG‑GGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCAATCGGcaca             <  2:50935/251‑1 (MQ=255)                                                                                                                                                                                                                         cGATCCTATCGAAACGGCAATCAAAAAAGG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCG‑GGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGccc  <  1:117562/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 CCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAG‑CGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCG‑GGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCC  >  NC_000913/1337150‑1337616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑