Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	36.2 / NA	15	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/5);  total (10/5)

ATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAG  >  NC_000913/1306536‑1306947                                                                                                                                                                                                         |                                                                                                                                                                                                                    aTCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGCGTGCGCCCAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGCTCCGCTTTAAAAACAATCAGGGCTgcc                                                                                                                                                                    >  1:40347/1‑248 (MQ=255)                         cGGCAGTACCCATACATGATCCGGATCTGGCGAAGAACAAACCCCTCCAGTTCCTGGACGGGGCATTACCGTCGCCGATCAACCCGCCTTCAGGTTGTGTTTTCCGTACACGTTGCCCGATTGCCGGTCCCGAGTGCGCAAAAACACGTCCTGTTCTGGCGGGGAGTTTCCGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACACTAAGGGATGACAGTTGCCAGACCTTTTTcccccta                                                                                                                                            >  2:50182/1‑244 (MQ=255)                                           aTCCGGATCTGGAGAAGAACAAAACCATCAAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGCCACGACGTTTCTTGCCTGAAAGTAGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGACCTTTTTCACGCAAAAAGCGATTGTTTATTccc                                                                                                                         >  2:179581/1‑251 (MQ=255)                                                gatgtGGAGAAGAACAAAACCATCAAGTTATTGGAAGGGGAATTACCGTCGCCGATCAACCTGCCTTCCGTTTGGGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGTCAAAACAGGTCCTGTTCTGGAGGGGAGTTTTAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGATTTAAAAAAAATAAGGGTTGACAGTTGGCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAg                                                                                                                    <  1:71582/247‑1 (MQ=255)                                                   cTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatg                                                                                                                 >  1:367981/1‑251 (MQ=255)                                                     ggAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACTTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAACAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTACAAGCGATTATTTATTCCCGCCAgatgat                                                                                                               >  1:10480/1‑251 (MQ=255)                                                            cccacccttctcatatcgtggtaaGGGGAATTCCCGTCGCCGATCAACCCGCCGTCCGTGTATGTTTCCCTGCCCCGTTGCCCGATGGCCGGTCCAGAGGTCGCCAAAACACGTCCTGTCCTGGAGGGGAGTTTCAGCCACGTCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGTGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATggaaa                                                                                                        <  2:133294/229‑4 (MQ=255)                                                                 aaCCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGTTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGttt                                                                                                   <  1:378196/251‑1 (MQ=255)                                                                 aaCCACCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGttt                                                                                                   <  1:271470/251‑1 (MQ=255)                                                                              cTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaa                                                                                                        >  1:133294/1‑233 (MQ=255)                                                                                             ccGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATg                                                                       >  1:191944/1‑251 (MQ=255)                                                                                                                    cGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGCGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTc                                                >  1:90075/1‑251 (MQ=255)                                                                                                                           gtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTAAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCCAAGATTGTGATCTTTTTCGAGGCATAAAAGAATGCGGGCAAAAACATCGTTGATTTCACCATCt                                         >  1:351434/1‑251 (MQ=255)                                                                                                                                                                  cccaaaaccatacctagtCTGGAGGGGATTTTGAGAAACGCCGTTTCTTGCCAGAAAGTCGATCCGCTTGAAAAACAATAATGGCTGACAGTTGTAAGCCCTTGTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCTCGGCATAACAGAATGCGGCCAAAAACAGCGTTGATTACACCATCTTCACTGTCCGCCAGACCAATCTCCTCGTCGGCAAAAAAg  <  2:152240/234‑1 (MQ=255)                                                                                                                                                                               cTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTc                                                >  1:343833/1‑192 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                                    ATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAG  >  NC_000913/1306536‑1306947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑