Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,282,857	C→T	R998R (CGC→CGT)	narG →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,282,857	0	C	T	96.7%	79.5 / NA	30	R998R (CGC→CGT)	narG	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/12);  minor base A (1/0);  total (18/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTG  >  NC_000913/1282617‑1283085                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     cTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCACTGAATAACGAAGACGAGAAGATCCGCTTCCGTGATATACAgg                                                                                                                                                                                                                            >  1:67853/1‑251 (MQ=255)              agCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATACAGAAGACGAGAAGATCCGCTTCCGAGATATTCAGGCACAGCCGCGCa                                                                                                                                                                                                                >  1:27266/1‑250 (MQ=255)                      ggtgcactgtcgaaatctcaccaacacCAAAGCGTAAGGTCCGGCGAAAGGCCAGTCTAGGCTGAACTCCGCGATTGAGTCGGCAGAGATGATCCTGACCCGGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCGGTGCTGCCCTGTGCTAATTTACCGGACGTGACCATACGCATCTGGCGCTGAATAAAGAAGAAGAGAAGATCCGCTTCCGTGATATTCAGTCTCAGCCGCGCAAAATTAta                                                                                                                                                                                                        <  2:58215/227‑2 (MQ=255)                                                  aaaGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGa                                                                                                                                                                           <  1:124897/251‑1 (MQ=255)                                                     gCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGa                                                                                                                                                                        <  1:136375/251‑1 (MQ=255)                                                           ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGt                                                                                                                                                                  >  1:354019/1‑251 (MQ=255)                                                                    aaaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGCAACCAACGGACAGGGAGCCGTGAAAGACTGGGCTGACCTGAACTAATTTAACGGTCGTCACCATACGCATCTGACGCTGCATAAAGAAGCCAACGAGATCCACTTCCGTGATATTCAGGCACACACGAGCCATATTATCTACAGCCCGGCCTGGTCTGGTCTAGAAGAAGCACACATTTCTTata                                                                                                                                                          >  2:111143/1‑248 (MQ=255)                                                                     aaGGCCAGCCGATGCTGAACACCGCAATGGATGCGGCAGCGATGATCCGTACCCTGGCACCGGAAACCAACGGTCAGGTAGCCTTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGTCACAGCCGCGCAAAATTATCTCCAGCCCGACCGGGTCTGGTCTGGAAGATGAACACGATTCTTACAAc                                                                                                                                                        <  2:117743/251‑1 (MQ=255)                                                                                    tGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGCAGCCGTGAAAGCCTGGGATGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTAGCGCTGAATAAAGAAGAAGAGAAGATCAGCTTCAGTGATATTCAGGCACAGCCGCGCAAAATTATCTCAAGCACGACATGGTCTGGTCTGGAAGATGAACACGTTTATTACAAAGCCGGTTacacaaa                                                                                                                                          >  2:220761/1‑247 (MQ=255)                                                                                     gAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAAc                                                                                                                                         <  1:70922/250‑1 (MQ=255)                                                                                                                       acacTGGCACCGGAAACCAACGGTCAGGAAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTTACCATACGCATCTGGCACTGACTAACGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATTTCCAGCCCGACCTGGTATGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCAGTGGCGTACGCTCTCTgg                                                                                                      >  2:109761/1‑251 (MQ=255)                                                                                                                         ccTGGCACCCGAAACCAGCGGTCAGGTAGCCGGGAAAGCCTGGTCTGCCCTGAGCGAATTTACCGGTAGTGACCTTACGCATCTGGCGCTGAATAAATAAGACGAGAAGATCCGCTTCCTTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc                                                                                                    <  2:129371/250‑1 (MQ=255)                                                                                                                            ggCACCGGAAACCAACGGTCAGGTCGCCGTGAACACCTGGGCTGACATGCGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATACAGAATACGAGAAGCTCCGCTTCCGTGCGATTCAGGCACAGCCGAGCAAAATTATCTCCAGACCGACGGGGGCTGGCCTGGCAGATGTCCAAGTATCTTACCACTCCGTTTACACCAACGATAACGAGATGATCACAtagcctcgccgtcctgctcgac                                                                                                 >  2:72198/1‑230 (MQ=255)                                                                                                                                 cGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGACCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCa                                                                                             >  1:323952/1‑250 (MQ=255)                                                                                                                                             gTCAGGTAGCCGTGAAAGCCTGTGCTGCCCTTAGCTAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAATACGAGAAGATCCGCTTCCGTGATATTCAGGCACATCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTTGAAGATGTACACGTTTCTTACAACTCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg                                                                                  <  1:366409/249‑1 (MQ=255)                                                                                                                                                             aGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGATGCATAAAGAAGACGAGAAGATCCGATTCCGTGATATTCAGTCACAGCCTCGCAAAATTATCTCAAGCCCGACCTGGGCTGGTCTGGAAGATGAACGCGTTTCTTACAACGCCGGTTACACCAACGTTCAAGAGCTGATCCCATGGCGTACGCTCTCGGGTCGACAGCAACAGTATCAGGAACACCAGTGAGTGCGTg                                                                >  2:201981/1‑251 (MQ=255)                                                                                                                                                             aGCCTGGGCGGCCCTGAGCGAATTTACCGGTCGTGACCATACGCAGCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGGCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGtt                                                                <  1:378600/251‑2 (MQ=255)                                                                                                                                                                            gAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc                                                  <  1:93552/250‑1 (MQ=255)                                                                                                                                                                             gatgacatttccGGTCGGGACCATACGCATCTGGCGCGGAAAAAAGAAGACGAGAAGATCCGCGTCCGTGATAGTCAGGCACAGCCGCGCAAAATTATCCCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACTAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCct                                                <  2:174386/241‑1 (MQ=255)                                                                                                                                                                                gAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAGTAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgct                                             >  1:355631/1‑251 (MQ=255)                                                                                                                                                                                    ttACCGGTCGTGACAATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                                         >  1:312236/1‑251 (MQ=255)                                                                                                                                                                                     tACCGGTCGTGACCATACGCATATGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATCTTCAGGCACAGCCGCGCAAAATTATCTCAAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGAAGCGTGATTTCGGTGAAAGCCTGCTGtttt                                        >  2:31651/1‑251 (MQ=255)                                                                                                                                                                                              tGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTTCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgc                               >  1:46906/1‑251 (MQ=255)                                                                                                                                                                                                     aCGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTTCACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCCGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGCTCg                         >  2:112701/1‑250 (MQ=255)                                                                                                                                                                                                                gcTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCgg             <  1:31651/251‑1 (MQ=255)                                                                                                                                                                                                                      tAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGaa         >  1:220046/1‑249 (MQ=255)                                                                                                                                                                                                                       aaaGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGaa         >  2:104140/1‑248 (MQ=255)                                                                                                                                                                                                                           aagaCGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGTTGAACACGTTTCTTACAACGCCGGTTCCACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGCCACCCGTTCGGTGAAAGAAGTg  >  2:312210/1‑251 (MQ=255)                                                                                                                                                                                                                                gagaAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTAAAAGCCTGCTGGtt                                         <  1:340490/207‑1 (MQ=255)                                                                                                                                                                                                                                gagaAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTAAAAGCCTGCTGGtt                                         >  2:340490/1‑207 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     CTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTG  >  NC_000913/1282617‑1283085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑