Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,253,002	C→T	intergenic (+17/+83)	dhaR → / ← ycgV	DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,253,002	0	C	T	100.0%	45.6 / NA	16	intergenic (+17/+83)	dhaR/ycgV	DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/11);  total (5/11)

CATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATA‑CTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGA  >  NC_000913/1252764‑1253227                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                   cATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCaga                                                                                                                                                                                                                        <  1:135806/251‑1 (MQ=255)                 aGGCGACAGATGATGTCATCGCCACCCGACTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTTATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCAt                                                                                                                                                                                                        <  1:66589/251‑1 (MQ=255)                       cAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGgc                                                                                                                                                                                                  <  1:188206/251‑1 (MQ=255)                             atggcaaccccACCCGCCTTTCCGCCTGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGTCGGTCGCATTCAGGAAATGTCGTCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACg                                                                                                                                                                                             <  2:344458/241‑1 (MQ=255)                                                                  tGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATc                                                                                                                                                        <  1:378109/250‑1 (MQ=255)                                                                        aGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAAAATGGCATTGATGCAGGGCAGTTTAAACGCCGGGGATGAAAGACAGAAACGATTTTTTATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACt                                                                                                                                                 >  1:91786/1‑251 (MQ=255)                                                                                              aaCGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATAtcctc                                                                                                                           <  1:338244/251‑1 (MQ=255)                                                                                                              caGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTg                                                                                                           <  1:18828/251‑1 (MQ=255)                                                                                                                               gAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATAtcctcc                                                                                                                          <  1:69287/219‑1 (MQ=255)                                                                                                                               gAAATATCGGCTTTACTTTGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGGTGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGGAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAACATCGTTTAAGGTCTTGTCTAACCCGCACTTACAAGATTACGCGATAtcctcc                                                                                                                          >  2:69287/1‑219 (MQ=255)                                                                                                                                           ttACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATcc                                                                               <  1:314838/250‑1 (MQ=255)                                                                                                                                                    aTCGGCCGCACTTCGCTGTGGCGGAAAATGAAGCAACATGGCATGGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCATCATCGTTTAAGGGCTTGTCTATCCCGCACTTaaa                                                                                                                                             <  2:5734/179‑1 (MQ=255)                                                                                                                                                    aTCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTaaa                                                                                                                                             >  1:5734/1‑179 (MQ=255)                                                                                                                                                                  gCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGt                                                                                                                                                             <  1:56756/149‑1 (MQ=255)                                                                                                                                                                     gtgGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCtgtttg                                                    >  1:37722/1‑251 (MQ=255)                                                                                                                                                                                                                       gggTATGAAAGACAGAAACGCTTTTTGAAACATCAGGGTGATCTGTATTTAATTACAGCGCGCGCTAACAATTTTAAGCATCGTGTAAGGGCTTGTCTATCCAGCGATAAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTAACATGCGTGTAATCAGCTTCGCGATAGAATGTGTGCAGTTTGTT‑TATCTGTTAACTTCCTCACACGCTGTTATTCAAGACATTACATTTGa  >  2:345993/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                   CATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATA‑CTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGA  >  NC_000913/1252764‑1253227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑