Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,227,656	G→A	intergenic (‑184/‑25)	pliG ← / → ycgL	inhibitor of g‑type lysozyme/PF05166 family protein YcgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,227,656	0	G	A	100.0%	45.9 / NA	20	intergenic (‑184/‑25)	pliG/ycgL	inhibitor of g‑type lysozyme/PF05166 family protein YcgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/6);  total (14/6)

GCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGA  >  NC_000913/1227413‑1227864                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                 gCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCCGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGAAGAATTCCAGTGGCTTTCCTGCGTTCGGAAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTCCTTATAAAGGGGGCATGGACTCCACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCggtt                                                                                                                                                                                                           >  1:178018/1‑249 (MQ=255) gCTCGTGGAGGATAGCAGTGCAAGCAGCAATACCGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCgggg                                                                                                                                                                                                           >  1:121060/1‑250 (MQ=255)  cTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGtt                                                                                                                                                                                                          >  1:88097/1‑251 (MQ=255)    cGTGGAGTTTAGCAGTGCAAGCAGCAATACAGCCTGCCTGATGCTCTTGTTTTGCATTGGGAGTCTCCTGTGCAGAATGAGTACGAGGAACGGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTTGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTGTTGCCGGTTGGATTACTTATAAGGGGTGCATGGACTTAACAAGCGGTTGAAATCGACAGCTGGCTGGCTTGTGACGTGGGtt                                                                                                                                                                                                          <  2:354987/249‑1 (MQ=255)                                      ttCCTGATGCTCTTGATTTTCATTGGAAGTCTCCTGTTCAGCATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGAGGCTTTCCTGCGGTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTTGCTGGCTTGGAACGAGGGTTTCATTGGTGTTAGCTTATTCCAAACCATGGCCTCtt                                                                                                                                                                      >  2:164067/1‑251 (MQ=255)                                                        ttCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGtt                                                                                                                                                    >  1:331942/1‑251 (MQ=255)                                                          cATTGGTAGTCTCATGGTCAGAATGAGTAAGAAGAACGGTTATAATTTAACAATCCCTAACGGGAGCATTCCAGTGGCTTTCCTGAGTTCGGAAAAATCACGTTGCAGCATTTTTGCCGGGGGGGTTACTTATTAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGACTTTTGACTCTGGATTCATTGATGTTAGTGTATGCCAAATCCTAGCATCTTACAGAGCaaacgaatggttt                                                                                                                                                  >  2:262322/1‑241 (MQ=255)                                                              ggTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTtgtgt                                                                                                                                              >  1:167354/1‑251 (MQ=255)                                                                         gTTCAGAATGAGTACGATGACCTGCTATAATATCACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCCCGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAACACGCAGAAATCTACAGCTGGCAGGCATGTGACGCGGGGTTCATTGATGGTAGCTTATGCCAAAACCTGGCATCtt                                                                                                                                                                      >  2:358843/1‑216 (MQ=255)                                                                         gTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCGGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCtt                                                                                                                                                                      <  1:358843/216‑1 (MQ=255)                                                                              gAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCTGTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAagcagc                                                                                                                              <  1:280615/251‑1 (MQ=255)                                                                                                gTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTATTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACTTAt                                                                                                            >  1:36361/1‑251 (MQ=255)                                                                                                                       gggAGAATTCCAGTGGCTTGCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGAGGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTAGCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGa                                                                                     <  2:167354/251‑1 (MQ=255)                                                                                                                                  aGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCgtg                                                                          <  1:82753/251‑1 (MQ=255)                                                                                                                                                       aaaaTCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTAAAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGaa                                                       >  1:223547/1‑249 (MQ=255)                                                                                                                                                            cACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGATGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAAACTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTACTGAGGAACTGATGAAAGGtttt                                                >  1:85154/1‑251 (MQ=255)                                                                                                                                                                                                aaGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGTCGATTTTGCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCATCCTCAGTTAGCGATGATTCTGCCGCTGGAt            <  1:179396/251‑1 (MQ=255)                                                                                                                                                                                                     ggCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCg       >  1:116957/1‑251 (MQ=255)                                                                                                                                                                                                         tGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGAGGGTTTCATTGAAGTTAGCTTATGCCAAAACATGGCATCTTCAAGAGTAAAAGTTTGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATCTTTCGCGTGTTCCTGAGGAACTGTTGAAAGGATTTGGTCAGCCTCAGTTAGCGATGATTCTGCAGCTGGAggggggta     >  2:6633/1‑245 (MQ=255)                                                                                                                                                                                                           gACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGACGCGGGTTTCATTGATGTTAGCTTATGCAAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTAAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaaga  >  1:43533/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                 GCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGA  >  NC_000913/1227413‑1227864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑