Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,201,550 C→A T18P (ACC→CCA)  ymfI → uncharacterized protein YmfI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,201,5500CA83.3% 53.3 / 1.7 24T18T (ACC→ACA) ymfIuncharacterized protein YmfI
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (10/10);  total (12/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

TGGGTATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGACCA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCTTAGT  >  NC_000913/1201313‑1201780
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
tgggtATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAG‑CCA‑AA‑AACA‑TTTga                                                                                                                                                                                                                             >  1:353008/1‑249 (MQ=255)
                             ccaaactttAACCCGCGTGCGGTGAGCCGCATCCACAACAACGCTAATGATGCTCTCTGGGTGAAAAGCCTTTACT‑TTCAGTTGCTGTCCTTTTCACCACTTCAGTCTCGGTGTTATGCTGGATTTCTCACACAGCCAGCAAGCAAGGAAACTTAAGGAACAAGTTATATGTTCACGTTCGTTTATTTGAAGACAAAGCCGAACAG‑CCA‑AATAA‑A‑TTTGTAGAATTAATGCTGAACTTGCTGTACCAGaca                                                                                                                                                                                              <  2:297084/244‑3 (MQ=255)
                                 aCCTTAACCCGCGTGCGCTGAGCCGCATTCACCCCAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTGATGTGCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAg                                                                                                                                                                                          <  2:320066/251‑1 (MQ=255)
                                  ccTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATAATTAGCTGTTATGTGCCTGTC‑TTTTCACCTCTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGt                                                                                                                                                                                         <  1:274199/251‑1 (MQ=255)
                                     tAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTa                                                                                                                                                                                       <  1:170296/250‑1 (MQ=255)
                                                              caccacAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCtgctgc                                                                                                                                                                >  1:120695/1‑248 (MQ=255)
                                                                          aaGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCag                                                                                                                                                   >  1:165972/1‑249 (MQ=255)
                                                                                                    tAGCTGTTATGTGCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGGCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAAt                                                                                                                          <  2:165972/248‑1 (MQ=255)
                                                                                                       cTGTTATGTGCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTg                                                                                                                    >  1:287848/1‑251 (MQ=255)
                                                                                                              gtgCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCcaaca                                                                                                              <  1:326194/250‑1 (MQ=255)
                                                                                                                    gTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACGTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCt                                                                                                        <  1:307013/250‑1 (MQ=255)
                                                                                                                         tttCACCACTTCAAGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCCGTTTTATGTTCACGTTCGTCTATTTGAAGACAAAGCCGAACAG‑CCACAA‑AA‑A‑TTTTAAGAATTAATGCTTAACTTTCTGCACCAGACACCAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCATAGGGAAATCTCCTCAAAAGCACAATGAACTGACAACCAATCATTGAt                                                                                                    >  2:290492/1‑250 (MQ=255)
                                                                                                                                    cAGGCTCGGGGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTc                                                                                         <  2:353008/250‑1 (MQ=255)
                                                                                                                                        ctcggaagtATGCTGAAGTTATCACACAGACAGCAAGCAAGTAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCAAGAGGGATATATCCTCAAAGGTACAATGAATTGCAAACAAATCCTTGATCAAAAATTTTCAATTg                                                                                    >  2:169056/8‑251 (MQ=255)
                                                                                                                                                            ctcACACAGCCAGAAAGCAAGGAAACTTTATGAACAAGTTTTATGCTCACGTTCGTATATTTGCAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TATGAAGAATTAATGCTTAACTTTCTGTACAAGAAAACATTTAAAGAGACTGACGATAGCTGCTGCAGACTGATTCCAGAGGACTATATCATCAAAAGTAAACTGAACTGCATCCTAATCCTTGATCAAACATTTTCAATTACTAAAAGAGCCggcgtagac                                                                >  2:77497/1‑244 (MQ=255)
                                                                                                                                                               actcagccagcAAGCAAGGAAACATAATGTAGCATGTGTATGTTCACGGTCGTCTATTTGAATACACAGACGAACAGACAA‑AA‑AA‑A‑TTTTAAAAATTAATGATTAACTTTCTGCTCCATAAAACAGTTAAAGAGTCTGACGATAGCGGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTTAATTTTTAACAGTGCCGGTGTTTACGCa                                                             <  2:324311/248‑1 (MQ=255)
                                                                                                                                                                    gccagcAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAatat                                                        >  1:23043/1‑251 (MQ=255)
                                                                                                                                                                        gcaagcaaGGAAACTTAATGAACCAGTTTTAGGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTTGCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAAGCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATAttt                                                    <  1:380775/251‑1 (MQ=255)
                                                                                                                                                                            gcaagGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCt                                                >  1:356209/1‑251 (MQ=255)
                                                                                                                                                                                   aaCTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAAtt                                         <  1:169056/251‑1 (MQ=255)
                                                                                                                                                                                          tGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACa                                   >  2:107358/1‑250 (MQ=255)
                                                                                                                                                                                                        gttcacggtagccTATTTGAAGGAACAGCCAAACA‑ACCA‑AA‑AA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAATGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCAAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGGGCCGGTTGTGACGCAAATATATTTTTCTGTAAATTGGACCAAAGCGCATGCTTACt                    <  2:100314/239‑1 (MQ=255)
                                                                                                                                                                                                              gTTCGTCTATTTGAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTc              >  1:352467/1‑251 (MQ=255)
                                                                                                                                                                                                                          gAAGACACAGCCGAACAGCCAA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCATCTGCTTCCTTAGt  >  2:211105/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
TGGGTATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTC‑TTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGACCA‑AA‑AA‑A‑TTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCTTAGT  >  NC_000913/1201313‑1201780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑