Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,194,922	G→A	T10T (ACC→ACT)	rluE ←	23S rRNA pseudouridine(2457) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,194,922	0	G	A	100.0%	12.1 / NA	6	T10T (ACC→ACT)	rluE	23S rRNA pseudouridine(2457) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/3);  total (3/3)

GTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATG  >  NC_000913/1194679‑1195125                                                                                                                                                                                                                                                    |                                                                                                                                                                                                            gTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTCGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTAtttt                                                                                                                                                                                                      >  1:176839/1‑251 (MQ=255)                                                     gcATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATg                                                                                                                                                    <  1:168877/249‑1 (MQ=255)                                                                                                                                                                                     actggaaggaCGTTGCGAGCTGGATCGCTTAACCTGGTGATTTCTAAACGAAGTTTTTTGCATAGTATATTCAGAGATTATGAATTGCCGCATTATAGCCTAATCACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGCCAAGCCAATTACAAATCATTAACAAAAAATTGCTCTCACGACTCCGTATCGCAGGACGCAAACGCATATGCAAGGTGGTAGCAGCCGAGAAACCCAGTcgcgatc                  >  2:363304/10‑248 (MQ=255)                                                                                                                                                                                            gAACGTTGTGAGCATAAGCGCTTAAACGGGTGATTTCTAAAAGAAGGTTTTTGAATAGTATTTTCAGAGATGATGAATTGCCGCATTATAGCCTAATACTGCGCATCTTTCATGACGGCAAACAATAGGGTTGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCGGTCGTAGCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGgag           <  2:302954/251‑1 (MQ=255)                                                                                                                                                                                                  ttctgcctgaaactcTTAACCTGGTGATTTCATAAAGATGTGTGTTGCTTAGAATTTTCAGAGATTATGAATTGCCGCATTATAGTCTAATAACGCGTATCTATCAGTACGGCAACCAATAGGGTAGTATTTACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCATCGGGGTGGCAGACGGGCAATCCAGTAGCGCTCGAAGGTGAGGta      <  2:94785/236‑2 (MQ=255)                                                                                                                                                                                                     gAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATAGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATg  >  1:204202/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                            GTTATTGGTCAGCACCAGCAACCCTTCGCTATCGCGGTCAAGGCGACCTGCTGCATAAACACCCTGAACCGGGATGAATTCTTTTAATGTTTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATCGCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATG  >  NC_000913/1194679‑1195125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑