Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,181,995	G→A	L279L (CTG→TTG)	potD ←	spermidine preferential ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,181,995	0	G	A	100.0%	34.3 / NA	18	L279L (CTG→TTG)	potD	spermidine preferential ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/7);  total (11/7)

GAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAGCAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCGGAGTTAAAGGCTGCCACGTTTGGCATCAGT  >  NC_000913/1181780‑1182227                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         gAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCACCGTCATTCTGCCATTCGCCATTTTTAATAGTTTCAGCATCCGGGAAGAGTGTTTTATCGTTCGCCACTTCTGGACTTAACAGCTTACGCTACTCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCAGGCGCAACAGGTGGTTGATCAATTTCAGCGCGCCTTCTTTGt                                                                                                                                                                                                       >  1:201207/1‑251 (MQ=255)                      cTTCTGATAATACTCTTCATAAATCCTGCTGGCTTCGCCAACGTCATTCTGCCATTCGCACTTTTTAATGGCTTCAGCATCCGGGCAGAGTGTTATATCGTTCGCCCCTTCTGGACTTAACAGCTTACGCGCCGCACGGTTTTGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCAAATACGGGCGCCACAGGCAGTTGATCAATTTCACCGCGGCTTCATTGTTATTAGCATTTGCCGGGaccg                                                                                                                                                                                  >  2:248508/1‑248 (MQ=255)                           ggtaataCTCTTCATTAATGCTCCTGGTGGCGCCAGCGTAATTCTGCCATTCGCCATTTTTAAGTGTTTCAGCATCCGGGTATAGTGTGTTATCGGTCGCCTCTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGG‑TTTGCCACATCCGGGCGCAACAGGAAGTTGATCAATTTCAGCGTGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCa                                                                                                                                                                               <  2:237487/247‑1 (MQ=255)                                            aTGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGTGCGCAACAGGCAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAAt                                                                                                                                                            >  1:1905/1‑251 (MQ=255)                                                        gcgcCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAACAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCcttct                                                                                                                                                  <  1:360953/249‑1 (MQ=255)                                                           ccAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAACAgg                                                                                                                                                                                                                                      <  1:154023/162‑1 (MQ=255)                                                           ccAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAACAgg                                                                                                                                                                                                                                      >  2:154023/1‑162 (MQ=255)                                                                 tCATGCTGCCATTCGCAATTTTTAATGGTTTCAGCATCCGGGTAGAGTGATTTATCGCTCGCCACTTCTGGACTTAACAGATTACGCGCCGAACGGTTTGGCGTTGGATAACCGATAGTTTAAGCAACCTGA‑TTTGCCACATCCGGGCTCGACAGGACGTTGATCAATTTCCGCGCGACTTCTTTGATTTTGGCTATTGCCGGGAGCGCCATGCTATCCCTCCGGAACATGACCCATTCTTCCGGacacac                                                                                                                                       >  2:77294/1‑251 (MQ=255)                                                                                     gttAATGGTTTCCGCATCCGTGTAGTGTTTTTTACCGTTCGCCACTTCTGGACTTACCATCTTACGCGCCGCAAGTGTTGGCGTTGGATTACCGATAGTTTCAGCATCCTGT‑TTTGCCACATCCTGGCGCAACAGGAAGTTGAGCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTTGCCATCCAGAAAATGCCGCCTTCTTTCTTCCGCACCACGTCAATTGGCGTAccc                                                                                                                    <  2:55548/249‑1 (MQ=255)                                                                                           gggttCAGCAACCGGGAAGAGTTTTTTATAATTAGCCACTTCTGGACTTAACAGCTAACGCACCGCAAGGTTTTTCGTTGGATAACTGATAGTTTCAGCAACCTGT‑TTTACAAAATCCGGGCGCAACAGAAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGACGTGATCGCACGGCGTTCCATCCAGAAAATGCAGCCTTCATTCGACCACACCCCGTCAATTggtataccctccgggc                                                                                                             >  2:368625/4‑237 (MQ=255)                                                                                                      ccGGGTAGAGTGTTTTATCGTTCGCCACTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAACAGGAAGATGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGACGGGATCGCCAGGCTGTCCATCCTGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGGGCAACg                                                                                                     >  2:104161/1‑248 (MQ=255)                                                                                                            agagTGTTTTATCGTTCGCCACTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAACAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTc                                                                                                                                               >  1:286818/1‑200 (MQ=255)                                                                                                                         gTTCGCCACTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAACAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGACAGCAGAAACGTTCCAGATc                                                                                >  1:182920/1‑250 (MQ=255)                                                                                                                                         cTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAACAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTc                                                               >  1:79038/1‑251 (MQ=255)                                                                                                                                             aCAGCTTCCTCGCCCCAAGGTTTGGCGTTGTATACCCGTTAGTTTAGGCAACC‑GTGTTTGCAACATCGGTGCGCTACAGAAAGTGGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCCCCATGTTGTCCATCCAGAAAAGGCCGTCTTCGTTCGGCCACCCCACGTCAATTGGCGTCCCCGCCTGGCGTGCAACGATAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCt                                                           <  2:380152/251‑1 (MQ=255)                                                                                                                                                    ccgcgCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACTTGT‑TTTGCCACATCCGGGCGCAACAGGAAGTTGATCAATTTCTGCGCTCCTTCTTGTTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATg                                                     <  2:303846/249‑1 (MQ=255)                                                                                                                                                                     gCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAACAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAGAGCAGAACCGTTCCAGATCATGCCGCGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGtt                                    >  2:152124/1‑250 (MQ=255)                                                                                                                                                                                                        tGCCACACCCGGGCGCAACAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTGGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCTCCTTCCATGTACGGGTTCGCCGGGTTATCGGAGTTAAAGGCTGCCACGTTTGGCATCAGt  <  1:332820/250‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         GAATTAACGTCCTGCTTTCAGCTTCTGATAATACTCTTCATAAATGCTGCTGGCTGCGCCAACGTCATTCTGCCATTCGCCATTTTTAATGGTTTCAGCATCCGGGTAGAGTGTTTTATCGTTCGCCACTTCTGGACTTAACAGCTTACGCGCCGCAAGGTTTGGCGTTGGATAACCGATAGTTTCAGCAACCTGT‑TTTGCCACATCCGGGCGCAGCAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACGAAAGCAGAACCGTTCCAGATCATGCCGAGGTTAACTTCGCCTTCCATGTACGGGTTCGCCGGGTTATCGGAGTTAAAGGCTGCCACGTTTGGCATCAGT  >  NC_000913/1181780‑1182227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑