Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,180,126	G→A	A203T (GCG→ACG)  A203T (GCG→ACG)	cobB → cobB →	protein‑lysine deacetylase/desuccinylase/lipoamidase protein‑lysine deacetylase/desuccinylase/lipoamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,180,126	0	G	A	96.9%	89.9 / NA	32	A203T (GCG→ACG)  A203T (GCG→ACG)	cobB cobB	protein‑lysine deacetylase/desuccinylase/lipoamidase protein‑lysine deacetylase/desuccinylase/lipoamidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (14/17);  minor base T (0/1);  total (14/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGATCGCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCT  >  NC_000913/1179880‑1180370                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ggCGATCGCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGtt                                                                                                                                                                                                                                                  <  1:234886/251‑1 (MQ=255)        gCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATg                                                                                                                                                                                                                                           <  1:304641/251‑1 (MQ=255)             ttGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCg                                                                                                                                                                                                                                       <  1:292784/250‑1 (MQ=255)                  tgTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTAAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAACTGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATAttt                                                                                                                                                                                                                                 >  2:81701/2‑251 (MQ=255)                  ggTGACGAAGAATATCGACAACCGGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGTTGAAAGTGCGTTGTTCACAAAGTTGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGatat                                                                                                                                                                                                                                   <  1:17864/249‑1 (MQ=255)                        gCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTg                                                                                                                                                                                                                           >  1:255314/1‑251 (MQ=255)                               atCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGGTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTgg                                                                                                                                                                                                                    <  2:26556/251‑1 (MQ=255)                                     aaCCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATc                                                                                                                                                                                                              <  2:145760/251‑1 (MQ=255)                                      cccTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTTAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATcc                                                                                                                                                                                                             <  2:255314/250‑1 (MQ=255)                                                        gTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCcggcg                                                                                                                                                                                           >  1:114434/1‑251 (MQ=255)                                                              ccAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTggg                                                                                                                                                                                     >  1:347682/1‑251 (MQ=255)                                                                           tatGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCAGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGAAGTTGTCGATGGCCGATATTTTCATTGCCATGGGCACATCCGGGCAGGTTTATCCGGCGGATGGGTTTGTTCACGatc                                                                                                                                                                        >  2:275564/1‑249 (MQ=255)                                                                                aTGGGGAACTGCTGAAAGTGCGTTGGTGATAAAGTGGTCAGGTACTCGACTGGTCAGGAGACGTTACCCCAGAAGATAAATGCCATTGGTGCCAGTTTCCGGCACCATTGCGCACGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTGTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGaaa                                                                                                                                                                   <  2:103054/251‑1 (MQ=255)                                                                                                        gttCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTggc                                                                                                                                            >  1:27393/1‑249 (MQ=255)                                                                                                             caAAGTGTTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGaa                                                                                                                                      <  1:171127/251‑1 (MQ=255)                                                                                                                                   ggACAGGTGACGTTACCCCAGAAGATAAATGCCATTGTTTCACGGTTCCGGCTCCCTTTCGCCCGCACGTAGTGTGGTTTGGCTAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTTATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGt                                                                                                                <  1:31428/251‑1 (MQ=255)                                                                                                                                           tacgttacccacgaagcaaactGCCATTGTTGCCAGTTTCCGGCACCCTTGAGCCCGCACGAAGAGAGGTTTAGCGCAAGGCCACTAGGCATGGTTGAACTTTATATGACGTTGTCGATGGCCGATATTATAATTGCCATTGTCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGCAGCGGAAATGCATTGAGAGACCACCGTGGAACTGCATCTTGTAACGAGTCAGTTAGGTAATGattt                                                                                                        >  2:22422/22‑248 (MQ=255)                                                                                                                                                 aCCCCAGAAGATTAATGCCAGTGTTGCCAGTTCCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATTCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCg                                                                                                   <  1:241040/250‑1 (MQ=255)                                                                                                                                                  ccccAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGAAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGACCTGAATCTTGAACCGAGTCAGGTTGGAAATGAATTTGCCg                                                                                                   >  2:300413/1‑249 (MQ=255)                                                                                                                                                               ttGCCCTTGTTGCCCGTATCCGGCACCCTTGTGCCCGCACGTCATGTGGTTTGGCGAAATGCCACTCGGCATGGATGAACTTTCTCTGACGTTGTCGATGGCCGATATTTTCAATGCCATTGGCCAATCAGGGCATGTTTTTCAGGCGGCTGTGTTTGTTCACGAAAAGAAACTGCATGGCGCGCACACCGTGATACTGTATCTTGAACTGCGACAGATTGGTAAGGAATTTGccacgaatcattacggac                                                                                    >  2:168631/2‑235 (MQ=255)                                                                                                                                                                       gttgCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAg                                                                             <  1:81701/250‑1 (MQ=255)                                                                                                                                                                              gTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGTAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGCACTGCATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAACAATTACGGCCCGGCAAGccgggggg                                                                     >  2:176091/1‑245 (MQ=255)                                                                                                                                                                                ttCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAggtggt                                                                    <  1:176091/250‑1 (MQ=255)                                                                                                                                                                                ttCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAggtggt                                                                    <  1:300413/250‑1 (MQ=255)                                                                                                                                                                                  ccGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTGCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGcc                                                                 <  1:126696/251‑1 (MQ=255)                                                                                                                                                                                         ccTTGCTCCCGCACGTAGTGTGGTTTGGCGAAATGACACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTATCATTGCCATGGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAGATGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGCGTCAGGTTGGTAATGAATTTTCCGAGAAATATTACGGCCCgggcaacccgggggtgaccaagatt                                                          >  2:337049/1‑228 (MQ=255)                                                                                                                                                                                              ccgctgCACGTAGTTGGGTGTGGCGAAATGCCACTCCGCATGGATGAAATTTATTTTACTTTGTCGATGGCCGATATTTTCTGTGCCATTGGCACACCCGGGCATGTTTATCCGGTGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTTGTGCCTGAGTTTGTTGa                                                     <  2:195615/246‑1 (MQ=255)                                                                                                                                                                                                               gTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGAGGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGGTTATCCGGCGGCTGGTTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGAt                                    <  1:275564/251‑1 (MQ=255)                                                                                                                                                                                                                                  ggcttGGATTAAATTGATAGGTCGTTGTCTATGGCTGATATTTTCATGGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTTGTGTTGTTCACGAAGCGAAACTGCAGGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGACAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATGAAAAGCGGGAAGCAtt                   <  2:183758/245‑1 (MQ=255)                                                                                                                                                                                                                                  ggCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGGTTAAAAGCGGGAAGCAtta                  >  1:183758/1‑249 (MQ=255)                                                                                                                                                                                                                                           gAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGACGGGATTAAAAGCGGGAAGCATTGCCTGATGCAc        >  1:351137/1‑251 (MQ=255)                                                                                                                                                                                                                                                 tataTGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGGGCCTGAGGTTGTTGAATAGTTGATGAAGGGGTTAAACGAGGTAAGCATTGCCTGATGCACTACGCt  >  1:197739/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GGCGATCGCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCT  >  NC_000913/1179880‑1180370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑