Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,974	C→T	A12V (GCT→GTT)	rluC →	23S rRNA pseudouridine(955/2504/2580) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,974	0	C	T	100.0%	51.8 / NA	18	A12V (GCT→GTT)	rluC	23S rRNA pseudouridine(955/2504/2580) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/11);  total (7/11)

CACTGCAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACT‑CACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGCTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGG  >  NC_000913/1144742‑1145207                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          cACTGCAAGTCTTGTTTTATCAATATGCCTTTTACCATTTATCACCCACTTACTTCATA‑TTTTTTAACTTCTTGCTGGTGATTGGTTTAAATACCTTCAATAAATTGATTACGCAGGGAAGGAATAAGGTGCATAATTAAATATAAGCATATTAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCTCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTTTTATCACCGCTGACGAAGc                                                                                                                                                                                                                         <  2:161027/251‑1 (MQ=255)              gtgataGCAAGATGATTTTTACCATTTATCATCCGCTTACT‑CTCAGTTTTTTCACTTCTGGCTGGTGAGTGGTTTAATAACAATCAATAAAGTGATCACGCAGTGAAGTAATAATGTGCAAAAGTAAATATAAGAATAGAAAAATTAGTGGCGCGAATCCTGTTGGCTAATTAGATGCGCCAACCATGAAAACAGAGTCTCCATCCGTAAAAATTGTTGTTATCACAGCTGACGAAGCGGGGCATCGTATc                                                                                                                                                                                                            <  2:206138/248‑1 (MQ=255)              gTTATAGTAAGATGACTGTTACCATTTAGCGCCCGCTTACT‑CACAGTTTTTTCACTTCGTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGTCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTAGCACCGCTGACGAAGCGGGGCAACGTAt                                                                                                                                                                                                             <  1:108370/250‑1 (MQ=255)                                                        cacaGTTTTTTCACGTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGGACCCAATTGAAAGGCGTACCaaa                                                                                                                                                                      <  1:111658/248‑1 (MQ=255)                                                                   cACTTCTTGCTGGTGATTTGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTAc                                                                                                                                                         <  1:382069/250‑1 (MQ=255)                                                                                    tGGTTTAATAACCATCAATAAAGTGATAACGCAGTGAAGTAAAAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGTGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAgg                                                                                                                                        <  1:280728/250‑1 (MQ=255)                                                                                                            gATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACg                                                                                                                <  1:116136/250‑1 (MQ=255)                                                                                                              tCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTAt                                                                                                             >  1:260236/1‑251 (MQ=255)                                                                                                                aCGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTa                                                                                                           <  1:106765/251‑1 (MQ=255)                                                                                                                  gCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTa                                                                                                           >  1:63926/1‑249 (MQ=255)                                                                                                                                        aaGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCgg                                                                                   >  1:193337/1‑251 (MQ=255)                                                                                                                                          gTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGa                                                                                                                                     <  1:211260/199‑1 (MQ=255)                                                                                                                                          gTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGAAGAAGCGGGGCAACGAATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGAATGATTTACCGTATTTTGCGTAAAGGCGa                                                                                                                                     >  2:211260/1‑199 (MQ=255)                                                                                                                                            aaaTAGAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATGAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGTGCAACGTATCGATAACGGTTTGCGTACCCAAGTGAAAGGCGTACCAAAAAGTAGGATTTACCGTAGTTTGCTTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCggggga                                                                               <  2:349058/251‑4 (MQ=255)                                                                                                                                                                        cgAATCCTGTTGGCTAATTAGAATCGCCAACCATGACAACAGAGACTCCATCCGTAAACATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGAATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGAGTAAAGGCGAAGTGCGGGTGAACAAACAACGAATTAAGCCTGCATATAAAATCGAAGCGGGTGGGGAGGTGCGTATTCCAcagagtagcgtt                                                   >  2:273391/1‑240 (MQ=255)                                                                                                                                                                                   cccctttcgatctctccaaccaaGAAACCAGAGACTCCATTCGTAAAAATTGTTTTTAGCACCGCTGACGAAGCGGGGCAACGTATCTATAACTTTTTTCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATGTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGga                                        <  2:259522/236‑1 (MQ=255)                                                                                                                                                                                                cGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGga                                        >  1:259522/1‑238 (MQ=255)                                                                                                                                                                                                                         aTCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTTCCCACATCTGCaaaaaggggggg  >  1:244005/1‑243 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          CACTGCAAGCCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACT‑CACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAGTGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGCTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAAAAGGTGGCGG  >  NC_000913/1144742‑1145207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑