Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,141,865	G→A	P835S (CCG→TCG)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,141,865	0	G	A	98.0%	142.8 / ‑4.5	49	P835S (CCG→TCG)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (24/24);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

CTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAAC  >  NC_000913/1141619‑1142105                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 cTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTTGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGAc                                                                                                                                                                                                                                              >  1:98809/1‑251 (MQ=255)   tcaggagtcgtcgaTTCAACCACTCCCGGTTGCGTTTCGGCAACCTGAACGGGGGCTTCTACTACACCGTCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGAc                                                                                                                                                                                                                                              <  2:106839/241‑1 (MQ=255)   tCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCTACCGTAACGCGGGCTTCTACGAGACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATATCGGCGTCGACAGGGACCTCAGTCACCATCCGCTGCACAT‑GTACTTCTTCCTGTTCGCGGTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGTATCCAGACTTTGCCAGAGGCCAGGTCCGAAGAcg                                                                                                                                                                                                                                             <  1:256721/250‑1 (MQ=255)     agtaTGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTCCGATAGCGGCGGCGACAGGGAACTCTTTCACCATCAGCTGCACAT‑GTACTTCTTCCTATTCGCGCTGCTCTTCCACATGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGACAGAGTCCAGTTCCGcagaggagc                                                                                                                                                                                                                                          >  2:298236/4‑246 (MQ=255)       gATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACATCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCTGACGTACAATTGGATAGCGGATACAGACTTTTCCAGAGGCCGATTCCGAAGACGCGCAc                                                                                                                                                                                                                                        >  1:63463/1‑251 (MQ=255)        aTGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGGCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGCCGCGCACg                                                                                                                                                                                                                                       >  1:280231/1‑251 (MQ=255)         tGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGc                                                                                                                                                                                                                                      >  1:30742/1‑251 (MQ=255)                                         ggcatccttcaccgggttttCTACGCCACCGGCCACTTCTTCAACAACTGGCTCTCTATCAGCCGGTGCGATAGCTGCGGCTACAGTGACCTAAGTCACCATCGGCTGCACAT‑GTACTTCTTCATGTTCTAGCTGCTCTTCAACCTGTACATCTTGCGGACGTAAAATTGTATAGCGGATCCAGGCTTGGCCAGAGGCCAGTTCCGAAGACGCTCACTCTTCTGTCAACGGCTTTGGTGACTGGGTTGGAt                                                                                                                                                                                                      <  2:230504/233‑1 (MQ=255)                                          gCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATa                                                                                                                                                                                                     <  1:213675/251‑1 (MQ=255)                                               cTGAACGGGGGCTTCTACGACACCAGCCAATTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAAACTGTACCTCTTGCGGGCGTACAATTGGATAGCGGATACAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCCAGCTACGGTCAACGGCACTGGCGACTGGGTTGGATAACGCt                                                                                                                                                                                                >  1:271339/1‑251 (MQ=255)                                                 gAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCg                                                                                                                                                                                              >  1:370456/1‑251 (MQ=255)                                                 gAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCg                                                                                                                                                                                              >  1:143828/1‑251 (MQ=255)                                                  aaCGGGGTCTTCTAGTACCCCGGCCACGTCGTCAACAACTGGCGCGCTAACAACCGTTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCg                                                                                                                                                                                              <  2:61508/250‑1 (MQ=255)                                                         gatgacacgacactgtgcaagtcttcAACAACGGGGGCGATAATAACCGGTGCGATAGCGGCGGCGACAGGGAACACAGGCACCATCGGCTGAACAT‑GGACTTTTGCCTGTTCGCGCTGATTGACAAACTGTACATCTTGCGGACGTACAATTGGATAGAGGATCGAGAATTTGCCAGAGGCCAGGTACGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGGTGGATAACGATCGTAACGATa                                                                                                                                                                                      <  2:125770/230‑1 (MQ=255)                                                           ttCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCAc                                                                                                                                                                                          <  1:292199/245‑1 (MQ=255)                                                               aCGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACAGCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGcgac                                                                                                                                                                                 <  1:144497/250‑1 (MQ=255)                                                                 gactcctgccccttcttCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTGCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCAGTGGCGACTGGGTTGGATAACGCTCGTCACGATAGcgacgac                                                                                                                                                                              <  2:199119/240‑1 (MQ=255)                                                                           aCTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCCACGGCATTGGCGACTGGGGTGGATAACGCTCGACACGCTAGCGACGACGACGCTGAcc                                                                                                                                                                    >  1:239768/1‑251 (MQ=255)                                                                                 tcAACAACGGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACGTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTa                                                                                                                                                               <  1:235229/250‑1 (MQ=255)                                                                                                  aacaacCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAgg                                                                                                                                               <  1:167244/249‑1 (MQ=255)                                                                                                             cGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGaa                                                                                                                                  <  1:236262/251‑1 (MQ=255)                                                                                                               aTAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACTGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGATGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAacg                                                                                                                                <  1:226510/251‑1 (MQ=255)                                                                                                                  gcgtcggcgACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCTGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACTCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAacgacg                                                                                                                             <  1:93138/247‑1 (MQ=255)                                                                                                                    gtcggcgACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGAcgcg                                                                                                                           >  1:319628/3‑251 (MQ=255)                                                                                                                         cgACAGGGGCCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAAGTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCCACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATg                                                                                                                      <  1:143029/251‑1 (MQ=255)                                                                                                                         cgACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATg                                                                                                                      <  1:85094/251‑1 (MQ=255)                                                                                                                            cagagcccTCAGTCACCATCGGCTGCACATTGT‑CTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCa                                                                                                                   <  2:143828/245‑1 (MQ=255)                                                                                                                            cAGGTACCTCAGTCACCATCTGCTGCACAT‑GTTCTTCTTCCTGTTCGCGCTGCTCGTCAACCTGTACATCTTTCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACTCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGAGAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACTCGGCATGCCa                                                                                                                   <  2:370456/251‑1 (MQ=255)                                                                                                                              gggACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGATGGATAACGCTCGTCACGATAGCGACGACGCCGCTGACCa                                                                                                                                                                   >  2:87211/1‑201 (MQ=255)                                                                                                                              gggACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGATGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCa                                                                                                                                                                   <  1:87211/201‑1 (MQ=255)                                                                                                                                     cAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTc                                                                                                           <  1:363246/250‑1 (MQ=255)                                                                                                                                     cAGTCACCATCGGCTGCACAT‑GTACTTCGTCCTGTGCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGt                                                                                                            <  1:223316/249‑1 (MQ=255)                                                                                                                                                              cttcttCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCtcttct                                                                                  >  1:192378/1‑251 (MQ=255)                                                                                                                                                                tcttcCAGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGCCGTACAATTGGATAGCGCAGCCAGACTTTGCCAGTAGCCAGTTCCGAAGACGCGCACGCTTCGGTCAACGGTATTGGCGACTGGGGTGGGTAACGCTCGTCACGTCAGCGACGTCGCCTCTGCCCACTTACGCGCAGGTGTCGTGAAGCGCGGCGAGAACGCCGGGGCATGCCACCCTTTGCACGTTTATCAGTGTTGTTCTctacttt                                                                                >  2:115683/1‑250 (MQ=255)                                                                                                                                                                       gTTCGCGCTGCTCTGCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACTGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTGCGCGCATTTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTcttctttgtgttc                                                                          <  1:64614/250‑8 (MQ=255)                                                                                                                                                                         tagcgcTGCTCTTCAACCTGTACATCTTGCGGAAGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCAGAAGACGCGCACGCTACGATCAACGGAATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACAACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCAACCGTTGACACGGTTATCAGCAATGTTATCTTACTGCTGTTCTgg                                                                       >  1:217927/3‑251 (MQ=255)                                                                                                                                                                         tCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGCCGCGGCATGCCACCGGTGGCCCGGTTATCAGCCTTGTTCTCTTCTTGCTGTTTTgg                                                                       >  1:217935/1‑251 (MQ=255)                                                                                                                                                                           gcgcTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGAGCAGGTGACGCGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTg                                                                     >  1:139759/1‑251 (MQ=255)                                                                                                                                                                            cgcTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGc                                                                    >  1:272419/1‑251 (MQ=255)                                                                                                                                                                            cgcTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGc                                                                    <  1:313026/251‑1 (MQ=255)                                                                                                                                                                             gctgctCTTCAACCTGTACCTCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGCGGACAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAAAGCTCGTCACGATAGCGACGACGACGCTGCCCACTTACGCGCAGGGAACGAGGCGAGCGGCGAACACGCCGCGGCATGCCACCCTTGTAACGGTTATCAGTATTATTCTCTTCTTGCTGTTCTggcgc                                                                    >  2:294929/1‑247 (MQ=255)                                                                                                                                                                                   ctTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGttt                                                               <  1:182063/249‑1 (MQ=255)                                                                                                                                                                                      cAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGAGCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAAGGCTCGGCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCCAGCCAGCGTTGTCACGGCTATCAGCATTGGTATCTTCTTGCTGTTCTGGTGCAGTTTGCGCt                                                          >  1:36431/1‑251 (MQ=255)                                                                                                                                                                                                   ttGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGCGAACGACGCGGCATGCCCCCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTTCGCTACGACTGGCAGCg                                             >  1:84499/1‑251 (MQ=255)                                                                                                                                                                                                    tGCGGAAGTACCATGGGATAGCGGAGCCAGACTTGGCGCTAGTCCAGTCCCGAAGACGGGCACGCTACGGTTAACGGCAGTGGCGACTGGGCTGGATAGCGCTCGTCACGATACGGAGGACGACGCTGACCACTTCCGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTTCAGTATGCGCTACGACTTGCAGCg                                             <  2:239768/250‑1 (MQ=255)                                                                                                                                                                                                      cGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGAAGACGAGCACGCTACGGTCAACGGCATTGGCGACTGGGCTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCggg                                           >  1:196048/1‑250 (MQ=255)                                                                                                                                                                                                                  tGGATATCGGATCCAAACTTTGCCAGAGGCCAGTTCAGACGACGCACACGCTACGGTACACGGCATTGGCGCCTGGGTTGGATAACGCTCGTACCGATAGCGACGACGACGCTGACCACTTACGCGACGGTCACGAGGCGATCGGCTAGAACGCGGCGGCAAGCAACCGTTGCAAAGGGTATCACAATTGGTCTCATCTTACTGTTATGGAGAAGTTTGCCCTACGCTTGGCAGCGGGACTTGCACCGGtt                              >  2:24368/1‑251 (MQ=255)                                                                                                                                                                                                                         cGGATCCAGAATTTACCAGAGGCCAGTTACGAAGACGCGCACGCCACGGTCAACGCCATTGGCGTCTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTAAGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACTGTTATCAGCATTGTTCTCTTCGTGCTGTTCTGGGGCCGTTTGCGCTACGACTGGACGCGGGCCTTTCTCCATTTCTGTGCg                       >  2:308928/1‑251 (MQ=255)                                                                                                                                                                                                                                aGACTTTGCCAGAGGACAGTTCCGAAGACGAGCACGCTACGGTCAACTGCATTGGCGACTGGGTTGGATAACGATCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGGGGCGAGCGGCGAGAACGAAGAGGCATGCCACCGTTGTCACGGGTATAAGCATTGTTCTCTTATTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGCCTTTAACCCGTTCTGTGCGTGGAGCt                >  1:374782/1‑251 (MQ=255)                                                                                                                                                                                                                                              gCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAAc  >  1:194217/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CTTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACAT‑GTACTTCTTCCTGTTCGCGCTGCTCTTCAACCTGTACATCTTGCGGACGTACAATTGGATAGCGGATCCAGACTTTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAAC  >  NC_000913/1141619‑1142105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑