Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,138,831 C→T R152C (CGC→TGC)  flgK → flagellar hook‑filament junction protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,138,8310CT100.0% 72.5 / NA 28R152C (CGC→TGC) flgKflagellar hook‑filament junction protein 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/11);  total (17/11)

AGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTA‑GAAGTCAGCGTTCAGG‑ATGGCGGCACTTATAACATCACGATGGCCAATGGT  >  NC_000913/1138598‑1139073
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
atgcacgtgtgtatcagaaccAAAGTAGAGGTCTTACTTCCCGATATGCGCAGATGTCGAAAGTCGACAATATACTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTTCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCATGCGCTGATTGGGAAATCAGAAGGATTGGTGAAGCAGTTTAAAACCACCGATCAATATCGGTGCTACCAGGACAAACAg                                                                                                                                                                                                                                     <  2:161170/231‑1 (MQ=255)
 gTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAgg                                                                                                                                                                                                                                    <  1:224299/251‑1 (MQ=255)
         cggcgCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAACAGATGCCGAAAAACGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATATCTTCACCAGACTGCAAACGATAGCGAGTAACGCGGAAGACCCGGCAGCGCGCCAAGCGGTCGTTGGGGAATCAGAAGGTTTGGTGGATACGATTATAACAACGGATCATTATCTGTGCAACAAGGACAAACAGGtaaaaatc                                                                                                                                                                                                                            >  2:239454/1‑244 (MQ=255)
              cAGACGCAAAGTAGCGGACTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTATAAAACCACCGATCAATATCAGTGCGAACAGGACAAACAGGTCAATATcg                                                                                                                                                                                                                           >  1:306433/1‑247 (MQ=255)
                                             agtaGCATATGTCGAAAAGCGACAATATGATCTCCCCCAGTCCCTCTCCGCGGGCAACACAGATGCCGGATTCCTTCCCCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTAc                                                                                                                                                                                        <  2:65638/248‑1 (MQ=255)
                                              tGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACg                                                                                                                                                                                       >  1:102699/1‑251 (MQ=255)
                                                aggagaggTCGACAATCGACATAATGCTCCTCACCAGTCCCTCTTCGCTGGCAACACAGATGCAGGATTACTTCACCAGTCTGCATACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCATTATCTGTGCGACCAGTACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACATCTACGCt                                                                                                                                                                                     <  2:372512/244‑1 (MQ=255)
                                                         cGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCAGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAAtt                                                                                                                                                                            <  1:2904/251‑1 (MQ=255)
                                                                               caccaGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAAtct                                                                                                                                                      <  1:87475/251‑1 (MQ=255)
                                                                                         tctTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGCTCAATATCTGTGCGACCCGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACAACGCTAACCAAATTGCAAGCCAGACCGATCAAATATCGAGCCTgcc                                                                                                                                            >  2:382086/1‑249 (MQ=255)
                                                                                                        caGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAACTTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTTGGGGCAgg                                                                                                                             >  2:119229/1‑251 (MQ=255)
                                                                                                                 gATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCAcc                                                                                                                    >  1:200468/1‑251 (MQ=255)
                                                                                                                                  gCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGa                                                                                                     <  1:59930/249‑1 (MQ=255)
                                                                                                                                      aCGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAAcg                                                                                               >  1:102001/1‑251 (MQ=255)
                                                                                                                                            gTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAAAAACTACGCTAAAAAAATTGCCAGCATGAACGATCAAATCACGAGCCTGAACGGCGTGTGGTCAGGGGCGTCAACTAACACTCTGCTGGAACAAcgcgcgc                                                                                          >  2:275777/1‑247 (MQ=255)
                                                                                                                                                     gcgGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAg                                                                                <  1:3527/251‑1 (MQ=255)
                                                                                                                                                                 gCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAAcc                                                                     >  1:332685/1‑250 (MQ=255)
                                                                                                                                                                  cAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAAcc                                                                     >  1:321624/1‑249 (MQ=255)
                                                                                                                                                                  cAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAAGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTCACAATCTGCTGTCTCAACGCGACCAACTGGTGAGCGAATTAAAca                                                                     >  2:136432/1‑248 (MQ=255)
                                                                                                                                                                                    ttGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCg                                                                                                                                                                                                            >  1:46239/1‑96 (MQ=255)
                                                                                                                                                                                    ttGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCg                                                                                                                                                                                                            <  2:46239/96‑1 (MQ=255)
                                                                                                                                                                                     tGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTA‑GCAGTc                                                >  1:379503/1‑250 (MQ=255)
                                                                                                                                                                                             cAGAAGGATTGGTAAATCAGTATAAAACCACCGCTCAATATCTATGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCATCGTTGATAAGATCAACAACTACGCTAAACAAATTGCCAGCCTGACCGATCAAATCACGCGCCTGACAGGCGTGGGGGCAGGGGCGACACCTAACAATCGGCTGGATCCACGCGATAAACTGGTGAGCGAATTAAACCAGAttgttgtaataaaagtatgcgttctg                                        >  2:200352/1‑231 (MQ=255)
                                                                                                                                                                                                      tGGTGAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACt                                                                                      >  1:193366/1‑196 (MQ=255)
                                                                                                                                                                                                      tGGTGAATCAGTTTAAAACCACCGAGCAATATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCGAACAATCTGCTGGATCAACGCGATCAACt                                                                                      <  2:193366/196‑1 (MQ=255)
                                                                                                                                                                                                          gAATCAGTTTAAAACCACCGATCAATATCTGTGCGACCAGGACAAACAGGTCAATATCACGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACATAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGCTTGTTGG‑GTAATACGACAGCGTTCAGG‑ATggcggacctt                         >  2:51410/1‑246 (MQ=255)
                                                                                                                                                                                                                            cGATCAATATCTGTGCGACCAGGACAAACAGGCCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACACATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGACAAGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGGGTA‑GAAGTCAGCGTT‑AGGTATGGCGGCACTTATAACATCACGATGGcc        >  2:106195/1‑250 (MQ=255)
                                                                                                                                                                                                                                 aaTATCTGTGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTA‑GAAGTCAGCGTTCAGG‑ATGGCGGCACTTATAACATCACGATGGCCAATGGt  <  1:37040/251‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
AGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGATTGGGAAATCAGAAGGATTGGTGAATCAGTTTAAAACCACCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATCGCGATAGGTGCCAGCGTTGATCAGATCAACAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTA‑GAAGTCAGCGTTCAGG‑ATGGCGGCACTTATAACATCACGATGGCCAATGGT  >  NC_000913/1138598‑1139073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑