Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,138,152 C→T T261I (ACC→ATC)  flgJ → putative peptidoglycan hydrolase FlgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,138,1520CT90.9% 52.4 / ‑3.0 22T261I (ACC→ATC) flgJputative peptidoglycan hydrolase FlgJ
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (8/12);  total (9/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

GGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAAT  >  NC_000913/1137917‑1138395
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   
gtttGGGGGAAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCGGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGGTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCGGGAAGCCTTGTCGGATGACGTTGGGCGGTTAACGCGTAACCCGCGCTCCGCCGCCGTGACGATCGCCGCGAGTGc                                                                                                                                                                                                                                         <  1:41977/246‑1 (MQ=255)
                                  gCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGACTCATGCAACTGGAAAGGGCCAGTTACTGAAATCCCCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTAt                                                                                                                                                                                                    <  1:360951/251‑1 (MQ=255)
                                                aaCCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTc                                                                                                                                                                                       <  1:66424/250‑1 (MQ=255)
                                                  ccTGGTTGGTTTCAAAGCCCCTGGCAACTGTAAAGTGCCAGTTATTTAAATCACCACGACTGAATATGAAAACGGCGAAGCTAAGAAAGTAAAAGCTAATTTTCGCGTCTACTGCTCGTATCTGGACGCCTTCTCGGATTACGTTGTGCTGTTAACGCTTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGTCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACt                                                                                                                                                                                    <  1:123919/251‑1 (MQ=255)
                                                              tcaaGCCTTTGGGAACTGGAAATGGCCATTGACTGAAAAGCCCGTGTCTGAATTTGAAAACGGCGGAGAGAAGAAAGTAAAAGCGAAGTTTTGCGTCTACAGCGCGTATCTGGAGTCCTTGTCGGGTTACGGTGGGCTGTTAACGCGTACCCCGCGCCGCGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCaaa                                                                                                                                                                         <  2:145207/248‑1 (MQ=255)
                                                              cAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAAc                                                                                                                                                                        <  1:272965/251‑1 (MQ=255)
                                                              cAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACAACGACTGAATATGAAAACGACGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCACGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGGACAGGGGGCGCAGGCCCTACAGGAAGCGGGCTATGCCACCGCTCCTCACTATGCCCGCAAAc                                                                                                                                                                        >  2:215692/1‑251 (MQ=255)
                                                                        ggCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACa                                                                                                                                                               <  1:314794/250‑1 (MQ=255)
                                                                                       ccAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGa                                                                                                                                                >  1:232653/1‑250 (MQ=255)
                                                                                         aGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGCCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTAACCAACATGATTCAGCAGATGAAAt                                                                                                                                             >  1:161170/1‑251 (MQ=255)
                                                                                                                       aaaCGGCGAAGCGAAGCAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCAACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTAc                                                                                                                >  1:331591/1‑250 (MQ=255)
                                                                                                                       aaaCGGCGAAGCGAAGAAAGTAAAAGCGAAGTTGCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACa                                                                                                               <  1:306508/251‑1 (MQ=255)
                                                                                                                             cgaagcgaagAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGAGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCCCCGATCCTCACTATGCCCGCAAACTCACAAACATGTATCAGCAGATGAAATCGAATAGCACCACGGTGAGCAAAACCTCAAGTATg                                                                                                          >  2:218936/1‑250 (MQ=255)
                                                                                                                                  ggaagaaAGTAAAAACGATGTATCGCGTCCACAGCTCGTTATTGGAGGCCTTGTCGGTTTCCGTTGGGCTGTTAACGTGAACCCCGCACTGCGCCGCCGTGATGATCGCCGCGATTGCGTAACAGGGGGCGCAGGCCCTACAGGACGCGGGTTATGCCACCGATCCTCACTATGCCCGCAAACTCTCTAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACAta                                                                                                    <  2:331440/250‑2 (MQ=255)
                                                                                                                                   gaagaaAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGCTTACGTTGGGCTGTTAACGCTCAAACCGCGCTACGCCGCCGTGACGACAGCCGCGAGTGCGGAACCGGGAGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAAATCACCAACATGATTCAGCAGATGAAATCGATAAGCGTCAAGGTGAGCAAAACCTACAGAATGAACAttt                                                                                                   >  1:362253/1‑250 (MQ=255)
                                                                                                                                    aagaaAGTAAAATCTAAGTTGGTCGCCTACCGCTAGTATCTTGAAGCGTTGTCGTATTCCGTTGGGCTGTGAACGCGTAACCCGCGCTACGCCGCCGTGACGCTCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATTCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGa                                                                                                  <  2:232653/251‑1 (MQ=255)
                                                                                                                                                  gAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGCTACGCGACAAGGTAAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGa                                                                                     >  1:281280/1‑250 (MQ=255)
                                                                                                                                                   aaGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAAt                                                                                   <  1:181535/251‑1 (MQ=255)
                                                                                                                                                                                           gATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGt                                             >  1:90252/1‑250 (MQ=255)
                                                                                                                                                                                                                        gcgcTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCTACAAGGTGAGCAAAACCTACATTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTc               <  1:243681/251‑1 (MQ=255)
                                                                                                                                                                                                                                 cgccgTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCCGGCCCTACAGGACGCGGGCTATGCCACCGCTCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCCAGTCCGGCGGGACGCTGCCGATAATACTCTGTAATTGAAGGCTTCTCAGGAACCTCCATGTCCAGCTTGAt      >  1:40777/1‑251 (MQ=255)
                                                                                                                                                                                                                                     gTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATtaat  <  1:119818/251‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   
GGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAAT  >  NC_000913/1137917‑1138395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: