Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,126,518	C→T	A121V (GCC→GTC)	yceH →	DUF480 domain‑containing protein YceH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,126,518	0	C	T	96.3%	74.6 / NA	27	A121V (GCC→GTC)	yceH	DUF480 domain‑containing protein YceH
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (15/11);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACCGTGAACCGGTCATGAATCTGAGTGAATCCGAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCG  >  NC_000913/1126282‑1126748                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       gAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATg                                                                                                                                                                                                                          >  1:267155/1‑251 (MQ=255)  aaCCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCCAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCCCCAAATATGGGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGATGCCCAGACGCCAGGTGAGCTGCGCAGCCGCGTCGCGCGAATGTATg                                                                                                                                                                                                                          >  1:81077/1‑250 (MQ=255)   aCCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGa                                                                                                                                                                                                                         >  1:106990/1‑250 (MQ=255)    ccGTGAACCGGTCATGAATCTGAGAGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAAt                                                                                                                                                                                                                       >  1:148025/1‑251 (MQ=255)       tGAACCGGTCATGAATATGAGTGAATCCGAAGTGCAGGAACAGCTGGCTAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGCTCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGGGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTc                                                                                                                                                                                                                     >  2:21165/1‑250 (MQ=255)           ccGGTCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTATAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCgt                                                                                                                                                                                                                >  1:348378/1‑250 (MQ=255)                         gAGTGAATCCAAAGTGCATGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCATCCGCGTCGCGCTAATGTATGAATTCAGCGATATGGCGGaa                                                                                                                                                                                                      <  1:322253/247‑1 (MQ=255)                                 ccaaaGTGCAGGATCAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGAc                                                                                                                                                                                           <  1:317846/247‑1 (MQ=255)                                                        aaTCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATcgcg                                                                                                                                                                    >  1:179679/1‑250 (MQ=255)                                                                             cTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGAACTTCTGACTTTGGCGATCTGAAACTGAGCGCAGCGGAATTGGAGTTAATCACCACGTTGTTATTGCGTGGTGCCCATACGCCAGAGGAACTGCGCAGCCGCGTCGCGAGGATGTATGAATTCAGAGATATGGCGGGAGTGGAGTGCACGATGGAAAAACAGGCCAAACGCGACGGTGGGCCTTTtgttggg                                                                                                                                                >  2:178217/1‑245 (MQ=255)                                                                                            ggTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAAcc                                                                                                                               <  1:638/251‑1 (MQ=255)                                                                                                ttGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCgggg                                                                                                                           >  1:170340/1‑250 (MQ=255)                                                                                                  ggTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTa                                                                                                                          <  1:113875/250‑1 (MQ=255)                                                                                                  ggTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGAGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTaa                                                                                                                         <  1:15074/251‑1 (MQ=255)                                                                                                          ggTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGaa                                                                                                                  >  1:325428/1‑250 (MQ=255)                                                                                                                                   tGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAgtg                                                                                         <  1:158580/250‑1 (MQ=255)                                                                                                                                      aaTTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGAGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGAAGCTGGGACAAATGGGAAAACCCGAAGATGGTCCTTTTGGGGTGCGTTTGGCCGACGAACCAGGAAAACGCGAACACCGCAAAATGCATCTTTTCAggggtgg                                                                                     >  1:123266/1‑250 (MQ=255)                                                                                                                                       aTTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGGAAACGCGAAAACCGCTACATGCATCTTTTCAgtgggg                                                                                      >  1:91775/1‑247 (MQ=255)                                                                                                                                               tttGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCttt                                                                                                                                                       <  1:267146/176‑1 (MQ=255)                                                                                                                                                   gCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAg                                                                        <  1:188782/251‑1 (MQ=255)                                                                                                                                                           aaaCTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGt                                                                <  1:169172/251‑1 (MQ=255)                                                                                                                                                                gAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGAc                                                             <  1:117114/249‑1 (MQ=255)                                                                                                                                                                gAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGGGACgg                                                           >  1:33229/1‑251 (MQ=255)                                                                                                                                                                          ggattGGCTTTAAGCACCATGTGGTTATTGCGTGGTGCCCATACGCCGGGTGCACTGCGCAGCCGCGTCGCGCTCATGTATGAATGCAGCGCTATTGCGGAAGTTGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTACTTTTGTGGTGCGTCTGGCGCGCGAACCGGGTAAACGCGAAAACTGCTATATGCAACTTTTCAGTGGTGAGGTTGAATATTATCCGGCGGTGACGGATATGTCGaa                                                 <  2:348378/247‑1 (MQ=255)                                                                                                                                                                                                tgttATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGGTATGTCGAACGCGGTTGACGGTGATTTAc                             >  1:12593/1‑249 (MQ=255)                                                                                                                                                                                                                         aGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGGGGAGTCGGCGCTGGAACAACTGGCAAATAGCGAAGAGGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAAACGCTACAGGCATCTTTTCAGTGGCGAGGTTGAAGATCAGCCGGCGGTGAAGGATAATTCGAACGCGGGTGACGGGGATTTACAGGCCAGCGTCGAAACCCTCGAAATCg  >  1:126495/1‑251 (MQ=255)                                                                                                                                                                                                                          ggTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGGTTTACAGGCCCGCGTCGAAGCCCTGGAAATCg  >  1:277144/1‑250 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       GAACCGTGAACCGGTCATGAATCTGAGTGAATCCGAAGTGCAGGAACAGCTGGATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCG  >  NC_000913/1126282‑1126748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑