Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,120,111 G→A A159V (GCG→GTG)  solA ← N‑methyl‑L‑tryptophan oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,120,1110GA96.7% 71.6 / NA 31A159V (GCG→GTG) solAN‑methyl‑L‑tryptophan oxidase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (16/13);  minor base C (0/1);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACA  >  NC_000913/1119871‑1120335
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                
gTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGACCAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGAt                                                                                                                                                                                                                        >  1:298156/1‑251 (MQ=255)
                       cACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGATGACCGGGCAGTTGAACAGTTGCGCGCAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGc                                                                                                                                                                                                 >  1:261858/1‑251 (MQ=255)
                               aGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCaa                                                                                                                                                                                          <  1:348987/250‑1 (MQ=255)
                               aGCGACCATAGGCCTGATATCAGGCAAATAATTTGCGTCCGGGCTGGACATGCAGCTCCGGGAGCAG‑GACTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCGGATACTCACCGCCAGCCGTTTCACTAGTTACTCCATCATCGTCATGCCGAATTTCGGGGTCCGGGAGGTAGAAAAGTTGCGCAACGCCCGCTTCCTTCACCAGTTGGCTCCAGGTCTTAATCGCCAGTTCgatgaccaa                                                                                                                                                                                          >  2:113445/1‑242 (MQ=255)
                                                 tctaggaaaaTACTTTGTGTACGGAATGGACAGGCAGCTCCGGGAGCAG‑CTATTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAACCGCTTCCATAGTTACGCCATCCTAGTCATGAAGACTTGAGGTGCACGGGCAGTTAAACAGTTGCGCACCTCCCTCTTACTTCACCGGATGAGTCCAGGTTTTAATCTCCAGTGCGCAGCACAAAACACCAGAATCAGTTTc                                                                                                                                                                        >  2:323724/8‑250 (MQ=255)
                                                    aGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCACAAAACCGGAATCAGTTTCAAAt                                                                                                                                                                    >  1:239251/1‑251 (MQ=255)
                                                            aCTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAG‑GTCTGTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGa                                                                                                                                                             <  1:237616/250‑1 (MQ=255)
                                                             cTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATg                                                                                                                                                           >  1:210062/1‑251 (MQ=255)
                                                                            gtactggccgCTCCGGGAGCAGTGT‑TGTGACCAATGTCCCCTCGCAGACAATCTATTTTGGCGCCTGATACTTACCGTCAGCCGGTGTAAGAGTTACGCCATCATCGCCATGACGAATTGCGGTGACCGGACAGTTGAACAGTTGCGCACATCCCGCTTCCTACACCAGTTTGATCCAGGTTTTAATCTCCCGTCTGCTGCGCATAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTGGTCCGGGaag                                                                                                                                            <  2:313249/242‑3 (MQ=255)
                                                                                  gCAGCTCCAGGAGCAG‑GTCTTGTACCCACGTTCCCGCGCAGACACTCGCTCTTATAGCCTGATACTCACCGTCAGCCGTTGTAATATTTACGCCATCATCGCCATGACGAATTGAGGTGACCGGGCAGTTGAACAGATGCGCTCAGCCCGCTTCCTTCACCAGTGGGATCTAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGTAATCAGCTTCAAATAATCCGATGTAGTTGTCCGGGACGCGTAtt                                                                                                                                      <  2:153941/251‑1 (MQ=255)
                                                                                    aGCTCCGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTc                                                                                                                                    >  1:130839/1‑251 (MQ=255)
                                                                                     gCTCCGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTcc                                                                                                                                   <  1:45094/251‑1 (MQ=255)
                                                                                        ccGGGAGCAG‑TTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCg                                                                                                                                  <  1:296306/249‑1 (MQ=255)
                                                                                         cGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGcc                                                                                                                               >  2:90207/1‑251 (MQ=255)
                                                                                               cAG‑GTATTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCggg                                                                                                                         >  1:116691/1‑251 (MQ=255)
                                                                                                          aCCCATGTTCCCTCTCAGACATACGCGTTTTTCGCTGGATGCCCACCGTCAGCCGTTTCAATAGTTATGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTACTTCCCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGTTGTGCAAAAAACCGTAATCAGTTGCAAATAAGCCGATGTAGTTTGCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATcc                                                                                                                <  2:302280/250‑1 (MQ=255)
                                                                                                           cccATGTCCCCGGGCAGAAAATCGCTTTTTTCGCCGGATCCGCACCGTCAGCCGTTTCAATAGGTACGCCATCATCTTCATGACGAATTGCGGTGACCGGGCAGTGGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCt                                                                                                              <  2:116691/251‑1 (MQ=255)
                                                                                                            ccATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCtt                                                                                                             <  1:178307/251‑1 (MQ=255)
                                                                                                                     cgcgCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGGTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTgcgactcg                                                                                                     >  2:282144/1‑245 (MQ=255)
                                                                                                                                                            aGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTggg                                                             >  1:22506/1‑251 (MQ=255)
                                                                                                                                                                 tttCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACg                                                        >  1:87114/1‑251 (MQ=255)
                                                                                                                                                                         gTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCg                                                  >  1:354214/1‑249 (MQ=255)
                                                                                                                                                                              gCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAAtg                                           <  1:105877/251‑1 (MQ=255)
                                                                                                                                                                              gCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAAtg                                           <  1:282144/251‑1 (MQ=255)
                                                                                                                                                                                   catcGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGaa                                      <  1:90207/251‑1 (MQ=255)
                                                                                                                                                                                    atcGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAAt                                     >  1:101944/1‑251 (MQ=255)
                                                                                                                                                                                     tcGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCAGCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATc                                    >  1:43335/1‑251 (MQ=255)
                                                                                                                                                                                          acgtcGAATTGCGAGGACTGGGCAGTTGAACAGTTGCGCACAGCCCGTTTCCTATGCCAATGGGATCCAGGTTTTAATCGCCTGTTCGCTGCGCAAAAAACCGGAATCAGTGTCAAATAAGTCGATGTAGTTGGTCGGGACGCGTAGTTCCGGCCAGCGTGCCATAATCCCGTGCGCAGCGAGCTTTTCAACGTTAAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCg                               <  2:92306/247‑1 (MQ=255)
                                                                                                                                                                                           tGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATACAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGGATCAGCCgg                              >  1:201350/1‑251 (MQ=255)
                                                                                                                                                                                                                  gTTGAACAGTTGCGCCCAGCCCGCTTCCTTCACCAGTTGGACCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGc       <  2:130839/251‑1 (MQ=255)
                                                                                                                                                                                                                       aCAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACa  <  1:172052/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                
GTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAG‑GTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAGTTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACA  >  NC_000913/1119871‑1120335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑