Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,115,185	C→T	G102G (GGG→GGA)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,115,185	0	C	T	89.5%	40.1 / NA	19	G102G (GGG→GGA)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/8);  minor base G (0/2);  total (9/10)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGCCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGAAGGGCATTACCAGACTGAAGGCGGCACCGGTAAGAAA  >  NC_000913/1114956‑1115421                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             cGGTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATg                                                                                                                                                                                                                         >  1:20771/1‑251 (MQ=255)  ggTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGc                                                                                                                                                                                                                        >  1:55946/1‑251 (MQ=255)                  ggACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGc                                                                                                                                                                                                           >  1:314006/1‑248 (MQ=255)                                                    cagccaTTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCg                                                                                                                                                                      >  1:310648/1‑251 (MQ=255)                                                     agccaTTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGCGTTTTCGGCCTTTacggacgg                                                                                                                                                                     >  1:75653/1‑251 (MQ=255)                                                                             acTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAgg                                                                                                                                             <  1:336197/251‑1 (MQ=255)                                                                                                                aGCCGCTTTTATTCCGCGGTTCTGGTGTGGCGATAAGATCAGTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTGTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACa                                                                                                          <  2:346069/251‑1 (MQ=255)                                                                                                                                gggTACTTGTGTGGCGAGAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCATTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATTCCGGACcacagat                                                                                          <  2:155638/250‑4 (MQ=255)                                                                                                                                                 tAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGAAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAGATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCg                                                                         >  1:375956/1‑251 (MQ=255)                                                                                                                                                    gagCATTAGCGTAGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGACCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTa                                                                      <  2:314006/251‑1 (MQ=255)                                                                                                                                                                tGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAgctg                                                           <  1:162440/250‑1 (MQ=255)                                                                                                                                                                   ggACAAATCCGCCAAGTACCCCACCAAGCGCCCGCAGGATCAAAAAACGCCAGATATTTTGTGCCTGTCCCTGCAACCCCATCACGCTGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTGCGGCCTGTACGGTTGGCGAGTCCTCCCCAAAACGGTGAGGCGATGGCCGACAATAAAAATGTAATGCTGAAGACAATGCCGGCCCACATATGCAGGGCGGAGTGTCCGGTAACGCCAAGCTGCTCa                                                       <  2:368261/251‑1 (MQ=255)                                                                                                                                                                               cAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACGGCCAGATAGTTGGTGCCAGGCCCAGCATCGCCATCACGATGCCAATGCCGAGGGCAGAGCGTAATAGCAGGAGTGTGCGGCCTTTACGGGCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAACTGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCGCAACGTAGAGGGGt                                           <  2:20771/251‑1 (MQ=255)                                                                                                                                                                                 aGTACCCCAAGAAGCGCCCGCAGGATCAAAAAATGCCAGATATTTTGTGCTAGGCCCAGCAACACCATCACGAAGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTGCGGCCTTTACGGTCGGCGAGTCCTCTCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATAGTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTaa                                         <  2:144462/251‑1 (MQ=255)                                                                                                                                                                                           gaagCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGAcc                                                                          <  1:33363/208‑1 (MQ=255)                                                                                                                                                                                                   cGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGAAGGGCATTACCAGACt                       >  1:336591/1‑251 (MQ=255)                                                                                                                                                                                                          tCAAAAACTGCCAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGCCCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGAAGGGCATTACCAGACTGAAggcg                >  1:207642/1‑251 (MQ=255)                                                                                                                                                                                                                     cAGATATTTTGTGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTCCGGTCGGCGCGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGCCAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGACGGGCATTACCAGACTGAAGGCGGCACCGGTa       >  2:162993/1‑249 (MQ=255)                                                                                                                                                                                                                        atatGTTGGGCCAGTCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGAAGGGCATTACCAGACTGAAGGCGGCACCGGTAAGaaa  <  1:54295/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             CGGTAATAAAGAATACCGGACGTAAGCCGTAGCTATCGGCGAGCAGGCCGCCAGCCATTGGGCCGAGCAACGCACCACTAACGCCGCCTGTGGAGAGCGTACCCAGCGCCCAGCCGCTTTTATTACGCGGTACTTGTGTGGCGATAAGAGCATTAGCGTTGGGGACAAATCCGCCAAGTAACCCAAGAAGCGCCCGCAGGATCAAAAACTGCCAGATATTTTGTGCCAGCCCCATCAACACCATCACGATGCCCATGCCGAGGGCAGAGCGTAATAGCATGAGTTTTCGGCCTTTACGGTCGGCGAGTCCACCCCAAAACGGTGAGGCGATGGCCGAAAATAAAAATGTAATGCTGAAGACAATACCGGACCACATATTCAGGGCGGAGTGACCGGTAACGCCAAGCTGCTCAACGTAGAGGGGTAAGAAGGGCATTACCAGACTGAAGGCGGCACCGGTAAGAAA  >  NC_000913/1114956‑1115421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑