Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,114,389	C→T	A368T (GCA→ACA)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,114,389	0	C	T	100.0%	91.9 / NA	33	A368T (GCA→ACA)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)

CAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGC‑AAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTACAGAAAAGATCAGCGCTGTAATCAGGATCTTTT  >  NC_000913/1114142‑1114628                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ccgCGTCAATGTCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGATGTTTCAGGGAAAAGGATAGCTGATTCTCCTCTTTTGCAAGTATGAAAGGCGAAAAAGCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATTGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGACACCGTAGTTCGCTGAAATCGCTGTTcc                                                                                                                                                                                                                                               <  2:277036/249‑1 (MQ=255)        aaTGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAAt                                                                                                                                                                                                                                        >  1:1566/1‑251 (MQ=255)                   cAAGCGTTGCGTACATTGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAAc                                                                                                                                                                                                                             <  1:316262/251‑1 (MQ=255)                            cGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGTGAAATACCGCTCTGAAACCGTAGTTCGCTGACATCGCTGTTCCCATCACTGGTCCGGTACCGTTGCCAat                                                                                                                                                                                                                    >  2:380172/1‑251 (MQ=255)                                cATGGACATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATc                                                                                                                                                                                                                 >  1:279755/1‑250 (MQ=255)                                        tcaCGATTCTCCCTTTGAGTTGATGCGGTTTCAGGGACAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTGGCCAATCTCACGAAACgc                                                                                                                                                                                                        >  1:266486/1‑250 (MQ=255)                                            gATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTgg                                                                                                                                                                                                    <  1:123318/251‑1 (MQ=255)                                                  cccTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAg                                                                                                                                                                                              <  1:146488/251‑1 (MQ=255)                                                         aGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGa                                                                                                                                                                                          >  1:38423/1‑248 (MQ=255)                                                               tgaGGATTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGCATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCATTATCACGAAACGATTGGTTATAGCTGAAGATACGcc                                                                                                                                                                                 >  1:330160/1‑251 (MQ=255)                                                                gaGGTTTCAGGAAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTCTGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGGAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGAGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCAAATATCACAAAACGATTGGTTATAACTGACGATACTccc                                                                                                                                                                                >  2:223836/1‑251 (MQ=255)                                                                           gAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGt                                                                                                                                                                     >  1:103614/1‑251 (MQ=255)                                                                                                             tGAAAGGCGAAAAATCAGTTCGATACCTGGGGCATTCGACGACGCCGTAGACTGATCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGCAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGCTACGCCCGGCGATCTGGTTGCTCGAGGTGTAAACCATCAGTGCATGTAcggc                                                                                                                                   >  2:158923/1‑251 (MQ=255)                                                                                                                     gAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGagta                                                                                                                           <  1:27033/251‑1 (MQ=255)                                                                                                                       aaaaTCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGagtagg                                                                                                                         >  1:83184/1‑250 (MQ=255)                                                                                                                         aaTCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATCGCTGACGCTACGACCGGCGATCTATGTGCTCGAGATGTAAACCAACAGTGTCTGTACGGCAGGGAGAAGTgg                                                                                                                       >  2:111180/1‑250 (MQ=255)                                                                                                                         aaTCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCAGGAATAGACTGCGGTGAATAACACTACGCCAGCGGTGACGAGAAATTCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCTGTAGCGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCTGCGAGCTGGTTTCGCGAGTTGTAAACCAACAGTGTCTGTACGGCGAGGAGTAGTGc                                                                                                                       <  2:340661/251‑1 (MQ=255)                                                                                                                          aTCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCa                                                                                                                      <  1:223836/251‑1 (MQ=255)                                                                                                                                tCGATACCTGGGGTATTCTACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCCCCATc                                                                                                                 <  1:280275/250‑1 (MQ=255)                                                                                                                                   aTACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGGGTAGTGCACCATcggcg                                                                                                             >  1:35640/1‑251 (MQ=255)                                                                                                                                          gggTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGGCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGa                                                                                                      <  2:37894/251‑1 (MQ=255)                                                                                                                                                                  gTTCCAAGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGGTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGC‑AAAAAACGTAAAATCCCAAg                                                                               <  2:193470/249‑1 (MQ=255)                                                                                                                                                                                  gCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCCCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACTATTGTTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGC‑AAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGaa                                                              <  2:363199/250‑1 (MQ=255)                                                                                                                                                                                       gAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGC‑AAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTaa                                                         <  1:26811/250‑1 (MQ=255)                                                                                                                                                                                       gAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGC‑AAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTaa                                                         <  1:158923/250‑1 (MQ=255)                                                                                                                                                                                         aTAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGCTCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGGAGGGGAACATCGGCGGCAACCAGCCAAAAAACGAAAAGCCCCAAGtgtcaagacggtcggcactaaga                                                        >  2:288417/1‑228 (MQ=255)                                                                                                                                                                                          tAACACTACGCCAGCGGGGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATAGGCGGCAACGGGC‑AAAAAACGTAAAATCCCAACTTGACATGGCGTCTggaagtaaagcc                                                     >  1:170822/1‑240 (MQ=255)                                                                                                                                                                                          aaaCACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGC‑AAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACa                                                     <  1:288417/250‑1 (MQ=255)                                                                                                                                                                                           taccttaagCCAGCGTGGACGAGTAATCCCGCTCTTAAACCTTCGTTCGCTGAAATCGCTTTTCCCATCAATGGTCCGGTAACGTTGCCAATATCCCGAAACGATTGTTTATAGCTGAAGAGACGCCCGGCGAGCTGGTTGCTCGCGTTGTAAACCTACAGTGTCTGTACGGCGGGGAGTAGTGCACCAGCGGCGGCACCGAGA‑AAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAAACa                                                     <  2:104364/242‑1 (MQ=255)                                                                                                                                                                                                            gACGAGAAATTCCGCTCTGAAACCGGATTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCACTATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCGCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGGAGTGCACCATCTGCGGCACCGAGC‑AAAAAACGTAAAATCCCAAGTGGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGt                                    <  2:35640/250‑1 (MQ=255)                                                                                                                                                                                                                       ccGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTc                                                                                                                                                                >  1:78930/1‑116 (MQ=255)                                                                                                                                                                                                                       ccGCTCTGAAACCGTAGTTCGCTGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTc                                                                                                                                                                <  2:78930/116‑1 (MQ=255)                                                                                                                                                                                                                                             tGAAATCGCTGTTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGC‑AAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTACAGAAAAGATCAGCGCTGTAATCAGGATCtttt  >  1:279798/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CAGCGTCAATGGCTTCTTCAAGCGTTGCGTACATGGTCATCACGATTCTCCCTTTGAGTTGATGAGGTTTCAGGGAAAAGGATAGCTGATTCTCCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGATTGGTTATAGCTGAAGATACGCCCGGCGATCTGGTTGCTCGAGTTGTAAACCAACAGTGTCTGTACGGCGGGGAGTAGTGCACCATCGGCGGCACCGAGC‑AAAAAACGTAAAATCCCAAGTTGCAATGGCGTCTGAACGTAAGACATTGGGATCAACAGCAGTACAGAAAAGATCAGCGCTGTAATCAGGATCTTTT  >  NC_000913/1114142‑1114628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑