Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,112,421	C→T	S520F (TCT→TTT)	opgH →	osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,112,421	0	C	T	100.0%	76.7 / NA	25	S520F (TCT→TTT)	opgH	osmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/10);  total (15/10)

GTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTG  >  NC_000913/1112190‑1112664                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    gtcaaaccttgccatatgttttCGGGGAAGCGGCGTTGAGGCGCCGCGCGGGGGGGGGGGTCTGGTTTGCTTACGACCCCCCGGGTTTTTATAAAAAATTCCCGCCAAATTTGTTTGATGAGCAAAAACG‑GGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTccgctccgct                                                                                                                                                                                                                                   <  1:266361/230‑1 (MQ=255) gTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCtg                                                                                                                                                                                                                                  >  1:301844/1‑251 (MQ=255) gTTCAATCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGACGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGAGGTGTTCCTGACGGGCGGGATGTTTAATCTCTccgctccgct                                                                                                                                                                                                                                   >  2:266361/1‑250 (MQ=255)     aaTCCTGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGt                                                                                                                                                                                                                              >  1:347432/1‑251 (MQ=255)          tGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTctccg                                                                                                                                                                                                                                          >  1:109500/1‑234 (MQ=255)                 ttttttggTGGAAGCGGGGTGGTGGCGCCGTGGTGGTGGGGGGGTCTGTATTTCTTACGATCCCCGGGGTTTTTAGGAAGTATTGCCGCCAAACTTGCTTGATGGATTAAAACT‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTTACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTc                                                                                                                                                                                                                   <  1:328455/243‑1 (MQ=255)                  aatttCGTGGAAGTTGCGTTGGTGCGCCGTGAGGGGTGGGGGTTCTGGATTGTTTACTTTCCCCGGGGTTTTTATGAATAATGGCCGCCTAATTTGCTTGATGAGC‑AAAACGGTCA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTcg                                                                                                                                                                                                                  <  1:358030/247‑1 (MQ=255)                                   gTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTCTGTTCCTCGCGCTCTCTACTTCATTGc                                                                                                                                                                                                >  2:110853/1‑251 (MQ=255)                                          cgcCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGtcgt                                                                                                                                                                                         >  1:94969/1‑248 (MQ=255)                                                             tCTGGATTGCTTACGATCTCCCGGGTTATTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCg                                                                                                                                                                      >  1:94009/1‑251 (MQ=255)                                                                       ttACGAACTCCCGGGTTATTATGAAGAATTGCCGACTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCAGATGGTGCCACGGTAACCTGATGAACTCACGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTGCCTGACGGGCGTGGTGGTGTATCTCTCCGCTCCGCTGTGGTTTATGTTCATGGCGCTGTCTACGACATTGCAGGTTGTGCATGCGTTGGCCGTACCGCAATCCTTcc                                                                                                                                                            >  2:109706/1‑251 (MQ=255)                                                                        tACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGAGTTGACCGAACCGCAATACTTCCt                                                                                                                                                           >  1:126543/1‑251 (MQ=255)                                                                            atccccggtgttttataaaaaattcgccgccTATCTTGCTTGTAGAGATAAATC‑‑TGAACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCa                                                                                                                                                        <  1:243386/225‑1 (MQ=255)                                                                                                                  tgttggatgggcaaaacggtgcccgccgATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGc                                                                                                                     <  1:131051/227‑1 (MQ=255)                                                                                                                              aaaCG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGAt                                                                                                     >  1:137627/1‑251 (MQ=255)                                                                                                                                        ccgccgATGGTGCCTCGGTATCCTGATGAACTTCCGTCTTTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACttt                                                                                             <  1:170056/251‑1 (MQ=255)                                                                                                                                                                   taaCTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTc                                                                   <  1:260936/249‑1 (MQ=255)                                                                                                                                                                          cGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGa                                                            >  1:13498/1‑250 (MQ=255)                                                                                                                                                                               gTTCTTGGTGAAGGGTAGGCACCCGGTTCACCGTGCGGTGTTACTGCCGGTCGTGATGTTTTATCTCTCCGCGCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCAGAAGt                                                         <  1:229179/248‑1 (MQ=255)                                                                                                                                                                                   cTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTg                                                    >  1:311373/1‑249 (MQ=255)                                                                                                                                                                                    tGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGc                                                 >  1:167675/1‑251 (MQ=255)                                                                                                                                                                                    tGGTGAAGGGTATGCACCCGATTCACCGCGCGGTATTCATGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGAATGCGTTGACCGACCCGCACTACTTCCTGCAACCACGGCAGTTGTTGCCAGTGTGGCCGCAGTGGCGTCGTGAGCGGGCGATAGCACTTTTTGATTTGTCCATGGTGCTGTTGTTTCTGCCGacgtgtttgagc                                                 >  2:119335/1‑240 (MQ=255)                                                                                                                                                                                                                     ggtTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTTTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGaa                <  1:222316/250‑1 (MQ=255)                                                                                                                                                                                                                                   gTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGCAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTg  >  1:183818/1‑251 (MQ=255)                                                                                                                                                                                                                                   gTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGATCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTg  <  1:337890/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    GTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGA‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTG  >  NC_000913/1112190‑1112664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑