Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,469	C→T	A711A (GCC→GCT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,469	0	C	T	89.7%	84.1 / 3.2	39	A711A (GCC→GCT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (14/21);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-01

ATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTAC  >  NC_000913/2224‑2710                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  atgaAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAggg                                                                                                                                                                                                                                              >  1:78538/1‑251 (MQ=255)                     ttcaggTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATg                                                                                                                                                                                                                           <  1:226501/246‑1 (MQ=255)                      tcaggTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGc                                                                                                                                                                                                                         >  1:17756/4‑251 (MQ=255)                           ttCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTg                                                                                                                                                                                                                                            >  1:84141/1‑227 (MQ=255)                           ttCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTATCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTg                                                                                                                                                                                                                                            <  2:84141/227‑1 (MQ=255)                              gCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGt                                                                                                                                                                                                                 >  1:114942/1‑251 (MQ=255)                                attcgtgtatCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGTCGCGGGAAATGGGTTAGACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTc                                                                                                                                                                                                                <  2:193580/243‑1 (MQ=255)                                 ttCTTATATATTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAAAGTTTAACTCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTca                                                                                                                                                                                                               <  1:60592/250‑1 (MQ=255)                                                  aGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCttt                                                                                                                                                                                             <  1:46332/251‑1 (MQ=255)                                                             ggCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGt                                                                                                                                                                                  <  1:114560/251‑1 (MQ=255)                                                                  gAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCg                                                                                                                                                                              <  2:114942/250‑1 (MQ=255)                                                                    gtttactcgatgcgacttctatGGCGCGTGAAATGGGTTATCCCGAACCGGATCCGTTGGAGGATCTGTCTGGTATGGATGTAGTGCGTAAACTATTGATTCTCGCTCGTGAAACGGTACTTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGATGGTGATGTTGCCGCTTTTATGGCGAAGCTGTCACAGCCCGACGATCTCTTTGCCGCGCGCGTGGTGAAg                                                                                                                                                                           <  2:203298/230‑1 (MQ=255)                                                                                 gactactcTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAg                                                                                                                                                              <  1:208696/244‑1 (MQ=255)                                                                                     actcTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGaaa                                                                                                                                                          <  1:349742/248‑1 (MQ=255)                                                                                      ctcTGGCGCGGTAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTGCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGc                                                                                                                                                                         <  2:259513/233‑1 (MQ=255)                                                                                      ctcTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGc                                                                                                                                                                         >  1:259513/3‑235 (MQ=255)                                                                                             gcgGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGATTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGGGGCGAAGGCCCGTGATGAAGGCAAAGTTTTgg                                                                                                                                                  >  1:10272/1‑250 (MQ=255)                                                                                                   aattggTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATg                                                                                                                                            <  1:264943/247‑1 (MQ=255)                                                                                                    aTGGGTTATACCGAACCGGATCCGCGAGATGATCTTTCTGGTATGGATGAAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCAGATTTTATGGCGACTCTGTCAAAGCTCAACGATCTTTTTGCCGCGCGCGGGGCGGAGGGCCGtggtgcagggaaggatttgggcattgt                                                                                                                                           >  2:1220/1‑226 (MQ=255)                                                                                                                                       ttCTGGTATGGATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGAGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGGGCCTGCCGCGTg                                                                                                         >  1:369989/1‑250 (MQ=255)                                                                                                                                                 gATGTAGCGCGTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCg                                                                                               >  1:85911/1‑250 (MQ=255)                                                                                                                                                           gTAAGCTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGt                                                                                    <  1:161573/251‑1 (MQ=255)                                                                                                                                                                 tATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGAtctc                                                                                                                                                                                                <  1:234213/137‑1 (MQ=255)                                                                                                                                                                 gaTTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTTAAATTGACCCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTTCCGCTTTTAGGGCGAATCTGTCACAGCTCGACGAactc                                                                                                                                                                                                <  1:234205/136‑1 (MQ=255)                                                                                                                                                                    tGATTCTTGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATcc                                                                            <  1:142373/250‑1 (MQ=255)                                                                                                                                                                                          acggtaatgtgtgctgggggtgattgaaatggaaCCTGTGCTGACCGCATTGTTTATCGCTGAGGGTGATGTTGCCGCTTTTATGGCGAAACTGTCACAGCTCGACGATCTTTTGGCCTCGCGCGTGGCTAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTAGGTTGGCAATATTGATGAAGATGGTGGCGGCCTCGTGAATATTGCCGAAGTGGATGGTAATGTTCCGCTGTTCAAAGTGAAATATGGCg                                                     <  2:365732/229‑1 (MQ=255)                                                                                                                                                                                                   ggAGCTGGCGGATATTGAAATTGAACCTGGGCTGCCCGCAGAGTTTAAAGCTGGGGGTGATGTTGCCGCTTTTATGGCGCATCTTGCCCAGCTCGACGGTATCTTTGGCGCGCGCGTGGCGAAGGCCCGTGATGACGGGAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTTCCGAAGAGGATGGGAATGATTCGATGGTCAAAGGGaaacagtgcgcaaaagcac                                            >  2:114044/1‑235 (MQ=255)                                                                                                                                                                                                     tccctacgctccgctctgccgatcttgtgCTGCTCGCAGCGTTTAACGCTGAGGTTGATTTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGTTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCt                                           <  2:316484/225‑1 (MQ=255)                                                                                                                                                                                                      gttggtggatgtggatattgaaCCTGTGTGGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGTCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTGTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAATTTTTGCGCTATGTGGGAAATATTGATGAAGATGTAGCCTGTCGCGGGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCTAAAACGCCCTgg                                         <  2:204573/235‑1 (MQ=255)                                                                                                                                                                                                      gCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCcgcg                                                                                                                                                                                       <  2:8866/109‑1 (MQ=255)                                                                                                                                                                                                      gCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCcgcg                                                                                                                                                                                       >  1:8866/1‑109 (MQ=255)                                                                                                                                                                                                        tGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGGAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCAGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCCAAGTGCAAAATGGCgcagatgggaagagg                                       >  2:18360/1‑237 (MQ=255)                                                                                                                                                                                                        tGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGa                                                    <  1:18360/238‑1 (MQ=255)                                                                                                                                                                                                         ggCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGcc                                       <  1:240931/250‑1 (MQ=255)                                                                                                                                                                                                                   gaaattgaaCCTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCTAATCTGTCACATCACGACGATCTCTTTGCCGCGGGCGTGGCGAAGGCTCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGTAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATGGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCa                            <  1:21744/251‑1 (MQ=255)                                                                                                                                                                                                                   gaaagtgaaCCTTTGCTGCCCGCAGAGTTCAACGCTGAGGGTGATTTTGCCGCTTTGATTGCGAATCTGTGACAGCTCGACGCTCTCTGTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTTCCGCGTGAAGATTGCCGAAGTGGATGGTAATGAGCCTCTGTTCAAAGTGGAAAATGGCGAAAACGCCCTGTCCTTTTATAGcc                             <  2:199118/250‑1 (MQ=255)                                                                                                                                                                                                                      attgaaCCTGTGCTGCCCGCAGAGATTAACGCTGAGGGTGATGTTGCGGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCACGTGATGGAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGGGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACt                          >  1:323574/1‑250 (MQ=255)                                                                                                                                                                                                                              tgtgCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAACGTGAAAAATGGCGAAAACGCCCt                                           >  1:316484/1‑225 (MQ=255)                                                                                                                                                                                                                                             gTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGtgc  >  1:16735/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTTCTGGTATGGATGTGGCGCGTAAACTATTGATTCTCGCTCGTGAAACGGGACGTGAACTGGAGCTGGCGGATATTGAAATTGAACCTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTAC  >  NC_000913/2224‑2710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑