Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,109,740	C→T	L136F (CTT→TTT)	opgG →	osmoregulated periplasmic glucans biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,109,740	0	C	T	96.7%	73.2 / NA	30	L136F (CTT→TTT)	opgG	osmoregulated periplasmic glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/12);  minor base G (0/1);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCG  >  NC_000913/1109497‑1109967                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    aaaTACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCCTTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTAccc                                                                                                                                                                                                                              >  1:360948/1‑251 (MQ=255)               tcagcagacctagttatattattaCAAAGAGTACTTGAACAATCTTAAGACCCCACTCCAACGTGTGTTCTACCATCAGGTTATGGTCTTAGATACCCCGGTTAAAATAAATGAAGTGACCTCCACCGCAGTCAAACGAAACAAATACAGCCCGGATTATTGCTTTTTCGGCGATGTTTAGCTTTACATAGATACGGTAAAAGACCTTGGCTTTGCCGTGTTTAAAGTGTTTTTCCCCATCAACAGCaaa                                                                                                                                                                                                                 <  2:240010/227‑1 (MQ=255)                               aataaTGAAAAAGCGGACTGTAACAAACTGATGACCCCATTCAAACCCGAGTTCTACCAGCAGGGTATGTACTTCGATACCCCGGTAAAAATAAATGAAGTGACTGCCACCGCAGACAAACGAAACAAATACAGCCCGGATTATGTCACTTTCGGTGATGTTCAGCATGACAAAGATACGGTAAAAGACCTAGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATTAACAGCAAAGATAAAAACGATGAAAt                                                                                                                                                                                                <  2:342581/247‑1 (MQ=255)                               aaacctgaaaaaGCGTACTGGAAAAATCTGAAGACCCCATTCAAACGCGAGTTATACCATCAGTGTATGTACTTAGAAACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGAACTTGGCTTTGCCGGTTTTAAAGTGTTTTTCCCGATCAACAGCAAAGATAAAAACGATGAAAt                                                                                                                                                                                                <  2:99656/242‑1 (MQ=255)                               aaTCATGACAAAGCGTACTGGAACAATCTGAAGTCCCCATTCAAACTCTAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAATACCTTGGCTTTTCCGGTTTTAAAGTGTTGTTCCCGATCAACAGCAAATATAAAAACGATGAAAt                                                                                                                                                                                                <  1:136888/251‑1 (MQ=255)                               aaTCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAAt                                                                                                                                                                                                >  1:342581/1‑251 (MQ=255)                                   aTGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTc                                                                                                                                                                                            >  1:88540/1‑251 (MQ=255)                                          aGCGTAATGGCACCATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGACGTGACTGCCACAGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGAATGACAAAGATAAGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACAAGATCAACAGCCAAGATAAAAACGATGAAATCGTAAGCATGc                                                                                                                                                                                     >  2:352956/1‑251 (MQ=255)                                                        aTCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCGCGGGGCCAGCTAt                                                                                                                                                                       >  1:248651/1‑251 (MQ=255)                                                                            cTCGAGTTCTACCATCAGGGTCTGTACTTCGATACCCCGGTCACAATAAATGAAGTGACTGCCACCGCAGTCAAACGACTCAAATAAAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGCCCTTGGCTTTGCCGGTTTTAAAGTATTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCACGCTATTTCCGCGTGCTTGGTGcagg                                                                                                                                                   >  2:158346/1‑251 (MQ=255)                                                                                         aTCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGc                                                                                                                                      >  1:100839/1‑251 (MQ=255)                                                                                                                                 aGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCAt                                                                                              <  1:152111/251‑1 (MQ=255)                                                                                                                                        gCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATAGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGATTATGGCCTTTCTGCCCGCGGCCTGGCAATTGTTACCGCCTTGCCATCGGGTg                                                                                        >  2:101353/1‑250 (MQ=255)                                                                                                                                                    aaaCGAATAAAATACAGCCAGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTGTGCTTTGCCGGTTTTAAAGCGTTTTACCCAATCAAACGCAAAGATAACAAAGATGAAATCGTAAGAATTCTACAGGACAGCTATTTCAGCGAGATTGGTGTAGGGCCGGTTTATGGCCTTTCTGACAGAAGCCTGGCAATTGATAGCGACTTGCAAACGAGTTAAGAAttttag                                                                             >  2:360977/1‑246 (MQ=255)                                                                                                                                                                        ggATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCg                                                       >  1:257396/1‑251 (MQ=255)                                                                                                                                                                               ttCACTTTCGGCGATGTTCAGCATGACCAAGATACGGTCAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTcc                                                >  1:102467/1‑251 (MQ=255)                                                                                                                                                                               ttCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTcc                                                >  1:254183/1‑251 (MQ=255)                                                                                                                                                                                        ggCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGAc                                       <  1:158346/251‑1 (MQ=255)                                                                                                                                                                                            aTGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCg                                                                                                                                                                                                                             >  1:276057/1‑65 (MQ=255)                                                                                                                                                                                            aTGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCg                                                                                                                                                                                                                             <  2:276057/65‑1 (MQ=255)                                                                                                                                                                                            aTGTACAGCATGAAAAAGAGATGGATAAAGCCCTCGGTTTTGCTGGTTTAAAATTGTGTTACCCGATCAACAGCAAAGACAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCATGTCAGGTGTATGGACTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTGCAAAGAGTGCTGGATCGAGTGTCCAAAACCGACTGAt                                   <  2:183713/251‑1 (MQ=255)                                                                                                                                                                                                tCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGACTTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGGCTGATaaa                                >  2:136978/1‑250 (MQ=255)                                                                                                                                                                                                 cAGCATGACAAAGTTACGGTAAAAGGCCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATAAAATCGTCAGCTTGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCACAACCGACTGATAAACg                              >  1:114097/1‑251 (MQ=255)                                                                                                                                                                                                 cAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCAGGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACg                              <  1:302410/251‑1 (MQ=255)                                                                                                                                                                                                              atgcGGTAAAAGACCTTGGCTGTTCCGGTTTTAAAGTGGTGTACCCGATCAACAGCAAAGATAAAAACGATGAAAGCGGCAGCATGCTCGGGGCTAGCTATTTCCGCGTGATTTGTGCAGGTCAGGTTTATGGCCTTGTTTCCCGCGGCTTGGCAATTGATTCCGCGTTGCCAGCGGGTGAAGAATTTCCGCGCGTCAAAGAGTTCTGTATCGAGCGTCCAAAATCGACTGAGAAACGTTTTACCaa                     <  2:365925/244‑2 (MQ=255)                                                                                                                                                                                                              aTACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCACAGATAAAAACGCTGCAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCAt                     >  1:365925/1‑247 (MQ=255)                                                                                                                                                                                                                   gTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCAtt            >  1:132731/1‑251 (MQ=255)                                                                                                                                                                                                                       aaGACCTTGGCTTTTCCGTGTTTAAAGTTTTTTACCCTAGCAACAGCAAATAAAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCTGGGTTAGGGCCTGTCTGCCCGCGGCCGGGTAATTGATACCGCCTTGCCTTCGTGTGAAGAATGTCCGCGCTTTAAAGAGTTCTGGATCGAGCGTCCAAAACTGACTGATAAACGTTTATCCATCTTTGTATTGCta        <  2:254183/251‑2 (MQ=255)                                                                                                                                                                                                                          ccattGGCTTTGCCGTTTTTAAAGGGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTTATTTGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCTTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTATCCATCTATGCATTGCTTGAc     <  1:173166/248‑1 (MQ=255)                                                                                                                                                                                                                              aggcgttGCCGGTTATAAAGGGTTTTACCCGATCAACAGCAAAGTTAAATACGATGAAATCTGCAGCATGCGCGGTGCCAGCTGTTTCCGCGTTATTTGTGCAGGTCTGGTTTATGGCCTTGTTGCCCGCGGCCTGGGAATTGAGTCCTCCTTTCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTGTGGATCTGGCGGCCAAAACCGACTGATAAACGTTTAACCTGGTATGCATTGCTTGACTcg  <  2:360948/245‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    AAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCG  >  NC_000913/1109497‑1109967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑