Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,107,209	C→T	I285I (ATC→ATT)	clsC →	cardiolipin synthase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,107,209	0	C	T	100.0%	79.6 / NA	26	I285I (ATC→ATT)	clsC	cardiolipin synthase C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/14);  total (12/14)

GGATCGCATCGAGTTACCCGCCTCCTGGCATAACGATGCCATGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATCTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTAACCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGCCGGATACGCGCGCTGGCGCAAAAAATTGCTCCGC  >  NC_000913/1106966‑1107365                                                                                                                                                                                                                                                    |                                                                                                                                                             ggATCGCATTGAGTTACCCGCCGCCTTGCATAACTATGCCATTATGCATCGTTATTTACTCAAAATGGAATCCAGTCAAGGTATAAATCATCGGGTTGATGGAACATTGCCGCTTATCTTGGCGAAGACTCGTTTATTAAGTGATGATCCGGCGAAAGGGTTGGTCAAGGCAAAACGGCATTCACTGTTTTCGCAGCGCCTGTTCGATTTCATGGGCTCACCCAGTGAACGCATCGATATGATTTCTTCCt                                                                                                                                                       <  1:244780/251‑1 (MQ=255)        aTCGAGTTACCCGCCTCCTGGCATAACGATGCCATGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGt                                                                                                                                                <  1:192084/251‑1 (MQ=255)            aGTTACCCGCCTCCTGGCATAACGATGCCATGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTGCCTATTTTGTACCg                                                                                                                                            <  1:149678/251‑1 (MQ=255)              ttACCCGCCTCCTGGCATAACGATGCCATGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGCTGGCACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGCGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGac                                                                                                                                          >  1:171009/1‑251 (MQ=255)                 cccGCCTCCTGGCATAACGATGCCATGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACAcg                                                                                                                                       <  1:282365/251‑1 (MQ=255)                          tGGCATAACGATGCCATGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGCTGGAACATTGCCGCTTATCTGGGAGAAGACACGTTTATTAAGTGATGCTCCGGCGAAAGGGGAGGGCAAGGCAACACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATAATGGGCTCACCCAGTGAACGCATCGCTATTATTTCTTCCTATTTTGTACCGCCACGCGCAGgtgt                                                                                                                              >  1:17093/1‑251 (MQ=255)                              aTAACGATGCCATGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGgcg                                                                                                                          >  1:31050/1‑251 (MQ=255)                                      gCCATGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCtt                                                                                                                  >  1:328859/1‑251 (MQ=255)                                         aTGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACg                                                                                                               >  1:377723/1‑251 (MQ=255)                                             cgcctCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGTTGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTCAGTGATGCTCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCCCTGTTACCGCAGCGCCTGTTCGATATCATGGGATAACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTCCCGACACGCGCAGGTGTGGCGCAACTCTTACGGATg                                                                                                           >  2:125791/5‑251 (MQ=255)                                                aTCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTg                                                                                                        >  2:144267/1‑251 (MQ=255)                                                          aCGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAggg                                                                                               <  1:144267/250‑1 (MQ=255)                                                              aaaaTGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGTGCGAAGACACGTTTATTAAGTGATGATCCGGCGCAAGTGGCGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCCTGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCTCAGGTGTGGCGCACCTCTTACGGCTGGTGAGAAAAgtgatac                                                                                           >  2:238105/1‑244 (MQ=255)                                                                        ccAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGAt                                                                                 <  1:346751/250‑1 (MQ=255)                                                                                 gtataAATCATCTGGTTGATGGAACATTGCCGCTTAGCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGGGAGAAAAGGGGTAAAGATTGCGATCCTAACCa                                                                         <  1:153985/248‑1 (MQ=255)                                                                                              ggatGATTGAACATTGCCGCTTATCTGGGCGAAGGCACGATTATGAAGGGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTACCCAATTCTCTTGcc                                                              <  2:31050/244‑1 (MQ=255)                                                                                              ggTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCTATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTAACCAATTCTCTTGCCGCt                                                           <  2:377723/250‑1 (MQ=255)                                                                                                            tGCCGCCTATCTCGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGCGGGCAAGGCAAACCGGCATTAACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGCACGCATCGATATTATTTCTTCCTATTTTGTACCGACACTCGAAGGTGCGGCGCAACTCTTACGGATGGTGCGAAAAGGGGTACAGATTGCGATCCTAACCAATTCTCTTGGCGCTAGCGCTGTTGCTgtt                                            >  2:147844/1‑250 (MQ=255)                                                                                                                        gggCGAAGACACGTTTATTAAGTGATGATCCGGCTAAAGTGGAGGTCAAGGCAAAACGTCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCGATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCTATCCTAACCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGCCgg                                 <  1:161842/250‑1 (MQ=255)                                                                                                                          gCGAAGACACGGTTATTAAGTGATGATCCGGCGAAAGGGGTGGTCAAGGCAAAACGGCATACACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTAACCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGTCGGATa                              <  2:328859/251‑1 (MQ=255)                                                                                                                                acacGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGCCACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTAACCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGCCGGATAcgcgcg                        >  1:372376/1‑251 (MQ=255)                                                                                                                                      ttattaAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGAGGGTGAGAAAAGGGGTAAAGATTGCGATCCTAACCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGCCGGATACGCGCGCTGgc                   <  1:125791/250‑1 (MQ=255)                                                                                                                                         ttaAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTAACCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGCCGGATACGCGCGCCGGCGCa                >  1:88672/1‑250 (MQ=255)                                                                                                                                                      cgtcGAAAGGGGATGGCAAGGCAAAACGGCAGTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATTTCGTCCTATTTTGTACCGACACGCGTAGGTTTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTATCCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGCCGGATACGCGCGCTGGCGCAAAAAATTGCTCCGc  <  1:343902/248‑1 (MQ=255)                                                                                                                                                                                                                                           cGATATTATTTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTAACCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGCCGGATACGCGCGCt                      >  1:331914/1‑146 (MQ=255)                                                                                                                                                                                                                                           cGATATTATTTCTTCCTATTTGGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTAACCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGCCGGATACGCGCGCt                      <  2:331914/146‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                             GGATCGCATCGAGTTACCCGCCTCCTGGCATAACGATGCCATGACGCATCGTTATTTACGCAAAATGGAATCCAGTCCATTTATAAATCATCTGGTTGATGGAACATTGCCGCTTATCTGGGCGAAGACACGTTTATTAAGTGATGATCCGGCGAAAGGGGAGGGCAAGGCAAAACGGCATTCACTGTTACCGCAGCGCCTGTTCGATATCATGGGCTCACCCAGTGAACGCATCGATATTATCTCTTCCTATTTTGTACCGACACGCGCAGGTGTGGCGCAACTCTTACGGATGGTGAGAAAAGGGGTAAAGATTGCGATCCTAACCAATTCTCTTGCCGCTAACGATGTTGCTGTCGTCCATGCCGGATACGCGCGCTGGCGCAAAAAATTGCTCCGC  >  NC_000913/1106966‑1107365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑