Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,090,508	C→T	E594E (GAG→GAA)	pgaA ←	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,090,508	0	C	T	100.0%	41.2 / NA	14	E594E (GAG→GAA)	pgaA	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/5);  total (9/5)

ACTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCT  >  NC_000913/1090271‑1090734                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                   tcttgcgtaaaaaTCATTGAAAGCCCATGAGCCATCGTACCTACGCCGCTCCTTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCATGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATTCTCCAGTTATCATTAAAATCATACCAGCCAGACAGGCTCGCGCCGGGTTTCTGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGc                                                                                                                                                                                                                        <  2:220844/242‑1 (MQ=255)      cGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGat                                                                                                                                                                                                                   >  1:103972/1‑250 (MQ=255)        gAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATAtt                                                                                                                                                                                                                <  1:232622/251‑1 (MQ=255)           aaaTCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACg                                                                                                                                                                                                             <  1:381387/251‑1 (MQ=255)                           aGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAac                                                                                                                                                                                              >  1:221628/1‑250 (MQ=255)                                   ccGTACTTCCGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCTTTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTCCCAGTGGCGAACCAATACGCCAGTTATAATTAAAATCATCCCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCCCGAGCCAGATTTTACGTGACCGCCACTCAACACCCGCAAg                                                                                                                                                                                     <  1:74974/251‑1 (MQ=255)                                          tACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGCACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTcg                                                                                                                                                                              >  1:345552/1‑251 (MQ=255)                                                        tGATACCAGCGAACATAAGCCTGAACACTGTTGCCTTTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTACAATTACGAACCAATACACCAGTTATCATTAAAATCATACCAGCCAGACCGGCGCGCGCCGGGTTTATGATCATGATTTAAACCGAGATCAGAGTATTCTACCTCGAGCCAGAGTTTACGTGACCGCTCCTCAACACACACAAGCAAGACGCGACCAATTCCttt                                                                                                                                                                >  2:186341/1‑251 (MQ=255)                                                                             tGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTCATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTCTCATTAAAATCATACCCGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAACCACGTTCCGCGCATTCTGCCTCGAGCCCGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTACCCTAAATTGCCCATc                                                                                                                                           >  2:112426/1‑251 (MQ=255)                                                                                                                                                                   gTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTAc                                                     >  1:179037/1‑251 (MQ=255)                                                                                                                                                                                 cATACCAGCCAGACAGGCGCGCTCCGGGTTTATGATCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACACGCAATCCAGTCACGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCCGCAAAAACGCGCCCGTTATCCTTCAGCGGTGGAGAATAAAAGTTGGTGGTTAAGTCGACATCCTGTTTACCACTATCCGGGCCt                                       >  2:242830/1‑251 (MQ=255)                                                                                                                                                                                    aCCAGCCAGACAGGCGCGCGCCGGGATTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGAGGTGGTGAATAAACGATAGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCg                                    >  1:279750/1‑251 (MQ=255)                                                                                                                                                                                                                     aatgtttaaaaaCGCGTTCTGCGTATTCTGCCCCGAGCAAAATATTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGATGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAg    <  1:134055/242‑1 (MQ=255)                                                                                                                                                                                                                      aTGATTGAAAACGCGTTCAGCGTATTCTCCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGAGAATAAACGATGGTGGTTAAGTCGCCATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGTTACGAAGcc  >  1:64633/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                   ACTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCT  >  NC_000913/1090271‑1090734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑