Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,086,153	C→T	G123R (GGG→AGG)	pgaD ←	poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,086,153	0	C	T	100.0%	49.2 / NA	20	G123R (GGG→AGG)	pgaD	poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/11);  total (9/11)

CGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGT  >  NC_000913/1085922‑1086396                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    cgcgtcttgggcaccGGATTAATGGAAGCGCTGGAACGTTTCGAGGAAGATGATATGGTTGGGAGCGTCCGGATCGGTAAATAGGACTGCGTACGTTCGGCACTGTGCCAACGTACGCTGCATGCCTACAGGTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGTGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAgtgt                                                                                                                                                                                                                                     <  2:334671/235‑1 (MQ=255)   cggcgTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGCAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTATTGTGTGTTTATTCCCGGACTAGCGCTTTTTATGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCt                                                                                                                                                                                                                                >  1:374247/1‑251 (MQ=255)           gCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGTGATCGTCCGGTTCGGTAAAGAGGACTGCGTTCGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGTGCGAACCTGGCTTTGTTTTGGGTGTTTATGCCCGGACGAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCTTCCtgt                                                                                                                                                                                                                        <  1:318058/251‑1 (MQ=255)           gCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCtg                                                                                                                                                                                                                         <  1:100730/250‑1 (MQ=255)                        tGACGCGCTGGAACGTTTCGCAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGACTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGGGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGGCCGCTCATCCTGTGGCTTTTTTGt                                                                                                                                                                                                             >  2:368489/1‑249 (MQ=255)                                            aagaagagtagactgtgtggtctgTCCGGTACGGTAAAGAGGACTGCTTACGTTCTGCGCTTTGCCCACTGACGCTGCAGGACTTCAGTTAAGTGTGTTATCGGTGAATAGCCCGGGCGTACCGGGTTTTGTGTTGGGTGTTTATGCCCGTACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTTGTTAAGTGTACGCTCAGTCTGTGTCTTTTTTGTAGTTTCTTATAGATCGCATCAg                                                                                                                                                                                       <  2:178736/228‑1 (MQ=255)                                                       aTGGTTGGGATCGTCCGGTTAGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTa                                                                                                                                                                              >  1:316910/1‑249 (MQ=255)                                                              ggATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTAGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTTGTGAAGTGTACGCTCATCATGTGTATTTTTTGTAGTTGCTGATAGAGCTCATCAGTTATTGCTAAGctctctt                                                                                                                                                                     >  2:366308/1‑250 (MQ=255)                                                                  cGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCat                                                                                                                                                                  >  1:72162/1‑250 (MQ=255)                                                                            ggTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTgcggc                                                                                                                                                       >  1:133499/1‑251 (MQ=255)                                                                               aaaGCGGACTGCGTACGGTCGGCACATTGCCAACGTACGCTGCCTGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTTTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGta                                                                                                                                                    <  2:66594/251‑1 (MQ=255)                                                                                                          tGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGctgctg                                                                                                                          <  2:104707/250‑1 (MQ=255)                                                                                                           gCCAACGTACGCTGAATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTTATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTgttgtt                                                                                                                        <  1:181351/251‑1 (MQ=255)                                                                                                               aCGTACGGTGCATGCCTACAGTTAAGTGTGTTAGCGGTGCAGAGCCCGGGCGATCCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGTCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTTTCTGCATATTCTTGCGGCGTATATTGGGAGTCTGCAGGATGCTGCTGTTTTTg                                                                                                                    <  1:314505/251‑1 (MQ=255)                                                                                                                                         tgtgtTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGACGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGccc                                                                                          >  1:380890/1‑251 (MQ=255)                                                                                                                                            gtTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGccc                                                                                          >  2:137518/1‑248 (MQ=255)                                                                                                                                                   gTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTaa                                                                                <  1:368489/251‑1 (MQ=255)                                                                                                                                                                                                       gcgcTTTTTCTGAAACAACCATTTTTATTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTg                            <  1:137518/251‑1 (MQ=255)                                                                                                                                                                                                                  taaaCATCCATGTTTATTTTCCTCTGTCTTGTGTAGCTTACGCTCATCCTGTGGCTTTTGTGTATTTGCTGATAGAGATCATCAGTTATTGCTAAGCTCTCTGTATATTCTTGCGGCGTATATTGGTAGGCTACATGATGCTGCTGTTTTTTAAAACGCTGCTAATTGTACAGCGCCAAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGTTTCTGGCCTCGCttt                  <  2:72162/249‑1 (MQ=255)                                                                                                                                                                                                                                 aTTTGCCTCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGt  >  1:1381/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    CGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAAGTGTGTTATCGGTGCAGAGCCCGGGCGAACCGGGCTTTGTTTTGGGTGTTTATGCCCGGACTAGCGCTTTTTCTGAAACAACCATTTTTATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGT  >  NC_000913/1085922‑1086396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑