Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,699	C→T	A372V (GCC→GTC)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,699	0	C	T	96.9%	84.3 / NA	33	A372V (GCC→GTC)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/15);  minor base G (0/1);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCTCTGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGT‑TTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTG  >  NC_000913/1066462‑1066936                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               aaGCTCTGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAAcc                                                                                                                                                                                                                                   <  2:5688/251‑1 (MQ=255)        ggTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAgg                                                                                                                                                                                                                            <  1:115395/251‑1 (MQ=255)                                  aaattaacgtgtTGGATGGGCACGACTCCCACATTGCCTCTATGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGCCCGAACGCACGCCGATCGGCGGCAAACTCGTTTTCCAGCGTTGGCAT‑GAAAGCAAAGCCCATCGCGATTTGATGCAAATTGAATATGTGAGA‑CAGCGTGCGGAACAGTTACGTAATGTCGAAGCGTTAACCCTGCAGGCCACTGAGCAGCGAGTGACGATGGa                                                                                                                                                                                                   >  2:352152/9‑250 (MQ=255)                                    aTTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAAtt                                                                                                                                                                                                >  1:320251/1‑251 (MQ=255)                                                     gcccGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGGATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAg                                                                                                                                                                               <  1:268602/248‑1 (MQ=255)                                                                                 aCGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGGCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATaa                                                                                                                                                   <  1:353572/251‑1 (MQ=255)                                                                                           agttCAAACCGTATCAGTTGCAGGACCAGAACGAACGAACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATAGA‑AGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑AAGAGTGAGGAACAGTTACGTAATGTCGATGAGTTAACCCTGCAGGCACATGCGCAGAGTGTGACGATGGAATTAAGCGGTAGCACGATAGAAGCTGATGGTGTCTGCAGGAGGGATAGGTTTGATAGc                                                                                                                                         >  2:282227/3‑251 (MQ=255)                                                                                            tttCAAACCTGATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACTCTGGAATTAAGCGGTTGCCCGATAGACg                                                                                                                                                                            <  2:240598/215‑1 (MQ=255)                                                                                            tttCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACg                                                                                                                                                                            >  1:240598/1‑215 (MQ=255)                                                                                               cAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGAGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGCTGACAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTTTGCACGGTGGATAAGTTTTATAggatgt                                                                                                                                     >  2:296701/1‑246 (MQ=255)                                                                                                           gTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAAGCGCGATTTGATGAAAATTGAATATGTGTAG‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCgg                                                                                                                         <  1:352152/251‑1 (MQ=255)                                                                                                             tGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTg                                                                                                                       >  1:42873/1‑251 (MQ=255)                                                                                                                  gACCAGAACGAACGCACGCCGATTGGCGGAAAAATAGTTTCCCAGCGTTGTGAT‑GACAGGAAAGCCAAGAGATATTTGATGAAAATTGAATGTGTGGAT‑CAGAGTTCGGAACAGTTACGTAATGTTGATGCGTTATCCCGGCAGGCACCGGCGCAGCGTGTGCCGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGGGAAAt                                                                                                                   <  2:208517/250‑1 (MQ=255)                                                                                                                         acgaacgCACGCCGATTGGCGGCAAAATCGTTTTCCCGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGCTGAAAATTGTATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCAGATAGACGCTGATGGATTCTGCCCGATGGATAAGATTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGaa                                                                                                            >  1:74082/1‑250 (MQ=255)                                                                                                                                cacgcCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcccc                                                                                                    <  1:128683/251‑1 (MQ=255)                                                                                                                                        ccgacgcccttccgatcttCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGaa                                                                                            <  2:127219/234‑1 (MQ=255)                                                                                                                                                      gTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCt                                                                              >  1:103609/1‑251 (MQ=255)                                                                                                                                                          tCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGaa                                                                                            >  1:127219/1‑233 (MQ=255)                                                                                                                                                           ccAGCGGTGGCAT‑GAAAGCAAAGCCAATTGCGATTTGATGAAAATTGAATATGTGGAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAATTAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTa                                                                         <  2:327166/251‑1 (MQ=255)                                                                                                                                                                     cAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGT‑TTTTa                                                              <  1:296701/251‑1 (MQ=255)                                                                                                                                                                               aaaGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAACCTCCCCCTCGAGAAGCGGGGGAATCGCTGTTTAAACGT‑TTTTACGTTCggtg                                                     >  2:303082/1‑248 (MQ=255)                                                                                                                                                                                        cgcgATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGCTAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGAGTTGAATGAAGCGGTGAAATAACa                                                                                                               >  2:211978/1‑185 (MQ=255)                                                                                                                                                                                        cgcgATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACa                                                                                                               <  1:211978/185‑1 (MQ=255)                                                                                                                                                                                                gatgaAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGTGCAGCGTGTGACGCTTGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGGTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGT‑TTTTACGTTCGATGGTCTGTTCGCCCCaaa                                     <  1:43586/250‑1 (MQ=255)                                                                                                                                                                                                    taaaTTGAATATGTGTATACA‑CGTGCGGCACAGTTACGTAATGTCGATGCGTTAACCCAGCAGGCACATGCGCAGCGTGTGACGCTGGAATTAAGCGTTTTACCGATAGACGCTGATGGTTTCTGCCCAATGGATAAGTTTGATAGCGTGATGAATGAAGCGGTGAAATTACAGATAACACCCCCACGAGAAACGGGAGAGTCGATGGGTAA‑CATATTTTACGTTCGAAGGTCCATTCGccaacaaaaatc                                >  2:332841/2‑241 (MQ=255)                                                                                                                                                                                                    aaaaTTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGAAATGACAATGCGTTAACCCAGCAGGCACCTGCGCAGAGTGTGACGATGGAATTAAGCGGTGGCCCGCAAGACGCTGATGGATTATGCCAGATGGATAAGTTTGAGAGCGTGTTGCATGAAGCGGAGAAATAACAGAAAACTGCACAGCGAGAAGCGGGGGAGTCGCTGGTTAAAGGA‑TTTTACGTACGAGGGTATGTTCGCCCCAAAAAAgg                                >  2:149244/1‑250 (MQ=255)                                                                                                                                                                                                      aaTTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGATAAACGT‑TTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCg                               >  1:36630/1‑250 (MQ=255)                                                                                                                                                                                                                        agagTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGT‑TTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCtt               >  1:230443/1‑249 (MQ=255)                                                                                                                                                                                                                                 aaCAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGT‑TTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCtt    <  1:322335/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGT‑TTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTg  <  1:315418/251‑1 (MQ=255)                                                                                                                                                                                                                                    cgTTACGTAATGTCGATGCGTTAACCCTGCCGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCACCCGCGAGaa                                                                                            >  2:335906/2‑161 (MQ=255)                                                                                                                                                                                                                                    aGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGaa                                                                                            <  1:335906/161‑1 (MQ=255)                                                                                                                                                                                                                                    aGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGT‑TTTTACGTTCGAGGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTg  >  1:20525/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               AAGCTCTGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCAT‑GACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTAT‑CAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGT‑TTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTG  >  NC_000913/1066462‑1066936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑