Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	780,624	C→T	noncoding (71/76 nt)	lysT →	tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,624	0	C	T	81.2%	61.2 / 3.1	32	noncoding (71/76 nt)	lysT	tRNA‑Lys
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base T (17/9);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTG  >  NC_000913/780386‑780860                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             gTAATGCATAACACACGACCAGAAGTCGCCTTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCt                                                                                                                                                                                                                                   >  1:299436/1‑250 (MQ=255)  tAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTACTCAATTGGTCGCAGGTTCGAATCCTGCACGACTCCCCAATCGCtc                                                                                                                                                                                                                                  >  1:360660/1‑249 (MQ=255)      gCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGAGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAACTCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGAACGCAGGGTCGAATCCTGCACGACTAACCAATCCCTAAGGTg                                                                                                                                                                                                                             >  2:324382/1‑251 (MQ=39)       cATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTgg                                                                                                                                                                                                                            >  1:89995/1‑251 (MQ=255)       cATAACACACGAACAGAAGTCGCATTATTTCTGGTCGTGACGAGCGCATAATAAGAAGTTGAGTGAACTACATCGAAATTTTTGTTGCGATCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGTATATCAAACAACCAGAAAGCAACGAAAAACAGG‑GTCGATAGCACAGTTGGTAGTTAAGATGACTTTTAATCAATTCATCGCCGAGTCGATTCCTGAACGaccaaccacatgctacgcta                                                                                                                                                                                                                             >  2:336542/1‑238 (MQ=255)              cacGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTCGTTACGGCGGAAGCGgt                                                                                                                                                                                                                     >  1:334146/1‑251 (MQ=255)                    cAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTaaa                                                                                                                                                                                                               <  1:188360/251‑1 (MQ=255)                           cGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGa                                                                                                                                                                                                         <  1:285603/250‑1 (MQ=255)                                                gtgCGAATCATACGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTACGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAAc                                                                                                                                                                                   >  1:97083/1‑251 (MQ=255)                                                    gAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGcc                                                                                                                                                                               <  1:152891/251‑1 (MQ=255)                                                           aaGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAgg                                                                                                                                                                         <  1:133390/250‑1 (MQ=255)                                                            aGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGCATAACGTTGCATGAGCAACGGCCCGAACGGc                                                                                                                                                                       >  2:76238/1‑251 (MQ=255)                                                               aGTTGAGTGATTTACAGCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGCCTTTTAATCAATTGGACGACGAGTCGAATCCTGCACGACTCACCAATAGCTAAGGTGTGCGCGGTAGGAAAAGGAGAAGAATAACGTAGCATGAGAAACGGCACGCAGGGCga                                                                                                                                                                     >  2:378715/1‑250 (MQ=255)                                                                      tGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGCGCAACGGCCCGAAGGGCGAGACGAAGt                                                                                                                                                             >  1:160842/1‑251 (MQ=255)                                                                              aTCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGGAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCAt                                                                                                                                                     >  1:245822/1‑251 (MQ=255)                                                                                         tgttgCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAc                                                                                                                                           <  1:325834/250‑1 (MQ=255)                                                                                                   aaGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACg                                                                                                                                             <  1:29703/238‑1 (MQ=255)                                                                                                   aaGTCTGAAATAAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACAGAAAGCAACGAAAAAGTGG‑GTCGTTATCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTTGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGAACGCGGTAGTCAAACGTGACGGAGAACGTTGCATGTGCAACGGCCTGAAGGGCAAGACGAAGTCGAGGCATCCTGCACGagatcgacagg                                                                                                                                  >  2:29703/1‑239 (MQ=25)                                                                                                            aTCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAACCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGAGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGACATCGTGCAAGACCCCACACTAACAtcgttagt                                                                                                                       >  1:370410/1‑251 (MQ=37)                                                                                                                        tGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAACGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTATGGAGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCAAGACGAAGTCGCGTCATCCTGCACGACCCACCACTACCCTCGTTAGTTGTAGTATcc                                                                                                             >  1:150533/1‑249 (MQ=25)                                                                                                                              cgtggCCACGGGATATCAAACAAACAGATAGCACAGTAAAA‑TGGTGTTGTAAGCTCAGTGGGTAGAGCAGTTGACTTTTAAATAATTTGTCGCAGGTTCTAATCCTGCACGACTCACCAATCTCTAAGGTGGAATCGGTAGTAAATCGTGAAGGATAACGTTGCATGAGCAACGGCCCTAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCgtagt                                                                                                     <  2:370201/247‑1 (MQ=21)                                                                                                                                          atatCAAACAAACCGCAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCTCTAAGTAGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATCAGAAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTCGAATCGGGTGAtt                                                                                          >  1:288308/1‑250 (MQ=25)                                                                                                                                          atatCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGAtt                                                                                          >  1:298214/1‑250 (MQ=255)                                                                                                                                                       cgaaacacatgAAAAAGTGC‑GTCGTTAGCTCAGTTGGTAGGGCAGTCGCCTTTTAATCAATTGGTAGCAGGTTCGAATCCTGCAAGACCCACCAATCGCTAAGTTGGAAGCGGTAGGAAAACGTGAAGAATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGgag                                                                            <  2:89995/241‑1 (MQ=25)                                                                                                                                                                          gggtcgtttggccggTTGGTGGAGCTGTGGCCTTTAAATCAATTGTCCTCCGGTTCGCAACCTGCACGACCCACCAATCGCTAATGTGGAATCGGTAGTAAAACGTATAGGATAACGTTGCTTGAGCAACGGCCCGAAGGGAGAGACGAAGTCGAGTCATCCTTCACGACCCTCCACTAACATAGTTAGTTGTAGTATCCAGCGAAGTATCGGGTGATTAGCTCAGCTGTGAGATCACCTCCCTGACAagg                                                          <  2:245822/237‑1 (MQ=255)                                                                                                                                                                                   gCTCATTTGGTAGAGCTGTTGACTTTTAATCAATTGGGCGCAGGTTCGAATCCTTCACGACTCACCAAGCGCTAAGGGGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACGAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcg                                                 <  2:299436/251‑1 (MQ=37)                                                                                                                                                                                                  cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGCCGAAGTCGAGACATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGCGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGttatatccctt                                  >  1:22133/1‑242 (MQ=255)                                                                                                                                                                                                        aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcatccc                            >  1:295834/1‑249 (MQ=255)                                                                                                                                                                                                        aCTTTTAATCAATTGGTCGCAGGTTCGAATCCGGCACGACTCACCAAGCGCTAAGGTGGAAGCGGTATTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGATACGAAGTCGAGTCATCCGGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcac                            <  2:295834/251‑1 (MQ=255)                                                                                                                                                                                                                  aaTTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCgg                  <  1:76238/251‑1 (MQ=255)                                                                                                                                                                                                                   aTTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGGTCCCGTCATCCCCCaccacccgg                 >  1:73926/1‑248 (MQ=255)                                                                                                                                                                                                                   aTTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCggg                 <  1:324382/251‑1 (MQ=255)                                                                                                                                                                                                                                  gAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGGTCCCGTCCTCACCCCCCACCGGGTCGTTAGCTCAGTTg  >  1:74654/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             GTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTGATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGG‑GTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTG  >  NC_000913/780386‑780860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑