Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 677,829 T→C W139R (TGG→CGG)  djlB → J domain‑containing protein DjlB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913677,8290TC80.0% 10.0 / ‑2.1 10W139R (TGG→CGG) djlBJ domain‑containing protein DjlB
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (1/7);  total (3/7)
Fisher's exact test for biased strand distribution p-value = 6.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

TAAATCTGTTTGGCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAA  >  NC_000913/677585‑678064
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           
tAAATCTGTTTGGCAACAAGTAGACTATGAGGAATCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACATGCATCTGATAGTGAACGTTTTCTGCCCTCCGCCTTGAAGCGCTTCATTCAACAATTAAATTATTGCTCGATGGTGGAGATTGATGAATTACGCTGGTCGCTGTGCACAACCGCCAGGAACAATGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGACGAAAGTTTGAGGCGGTTAc                                                                                                                                                                                                                                       >  2:341706/1‑251 (MQ=255)
                ccAGAAGAATAGGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAAGCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCgg                                                                                                                                                                                                                       <  1:222871/251‑1 (MQ=255)
                     agaaTATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCggcggtatcatggggataaaaacgggg                                                                                                                                                                                                                     >  1:162932/1‑227 (MQ=255)
                                                            tCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCAtt                                                                                                                                                                            <  1:353123/250‑1 (MQ=255)
                                                                         gCCTCTGATAGTGAACGTTTGCTTACCTCCGCCTGGCTGCGATTCATTCAGCAATTTAATTATTGCGCGATGGAGGAGATTGATGAAGTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGATGAAAATACCGGGGAAATAGACGAAGAAGATCTGGAATCCTTTTTATATGCCCTGGCGTAGAGGAATg                                                                                                                                                               <  2:201905/250‑1 (MQ=255)
                                                                                                                                             gatagaggagATTAATGAATTACGCTTGTCGTTGTGAACAAACGCCATGAACACTCCCCATTTATCCGTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCTGCGGTTACAGGAGGAAAATACCGGGGAAATAGTCGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAAGGTTTTCAACTGCCAGACCATTCTGCATCTGCTCGTTGCCGTGCTAAATGACACCATCGATTTTTACCaaa                                                                                          <  1:232449/247‑1 (MQ=255)
                                                                                                                                                ggaggaGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCa                                                                                                                    <  1:511/222‑1 (MQ=255)
                                                                                                                                                                        gTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTg                                                                                                                                  <  2:259832/184‑1 (MQ=255)
                                                                                                                                                                                     aTCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTc                                                                       <  1:319190/230‑1 (MQ=255)
                                                                                                                                                                                        gCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACAtt                                               >  1:183353/1‑251 (MQ=255)
                                                                                                                                                                                                                                      cAGAAAGATTGCGGTGGTTACCGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCCTCGATTTTCACCAAATGTTCGATCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGCTGCaaa  >  2:30960/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           
TAAATCTGTTTGGCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAA  >  NC_000913/677585‑678064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑