Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,259	G→A	C175C (TGC→TGT)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,259	0	G	A	92.6%	65.7 / ‑3.2	27	C175C (TGC→TGT)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (15/10);  total (17/10)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

AAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCC  >  NC_000913/674026‑674493                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           aaGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTg                                                                                                                                                                                                                           <  1:112504/251‑1 (MQ=255)   ggTGTCCGGGCGGGAAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTgtt                                                                                                                                                                                                                         >  1:96622/1‑251 (MQ=255)                      tAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTg                                                                                                                                                                                                      >  1:193626/1‑251 (MQ=255)                                 cGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGACTTCGGAAAGACCGATCCAGATACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTAACCAGATCGATCAGCCGCCCGTCAGCGTAAGCAGTGATTTGGATACACCACAGCGGGATCTCTTTACGTTCCACTTTGGTATCTCAGCGCCAGCAACAGCAGTCGATACCTTGTTCGTACGCCAGTACGGTCTGCTCGtgagggc                                                                                                                                                                                           >  2:220292/1‑245 (MQ=255)                                    gttgtCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGCTTTTGATAAACCACTGCGGGGTCTCTTTACGTTCACCTTTGGTATCGCCTCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCATTACGGTCTGGTCGTTCgtgcccc                                                                                                                                                                                        >  2:282363/1‑245 (MQ=255)                                          aTAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGa                                                                                                                                                                                  <  1:308216/251‑1 (MQ=255)                                               cGTTAACGTTGAAGATGATATCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCATCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGcg                                                                                                                                                                             >  2:125771/1‑250 (MQ=255)                                                                          cgtgggAACGACCGATCCACTTACGCTGCATGGTTTTAACGGAGTCTGCAAAGTGATACAGATTATACAGATAGTTGAGCAGCTGTTCAGCGTAAGCAGTGATTTTTATAACACACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGACCGCAACAGCAGTCACTATCTTGCTCGTTCGCCAGCACGGACTGGTCTTTCGGGCACCCGTGGTCCGCAGAAGTCGTCTTCTATAccgtgtctttt                                                                                                                                                  >  2:104077/5‑242 (MQ=255)                                                                             cgtaatgACCGATCCAGCTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCTGATCGTTGAGCCGCTCGTCAGCGTAAGCAGTGTTTGTGATAAACCAATGCGGGATCTCTTTTCGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATATCTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTta                                                                                                                                               <  1:263068/245‑1 (MQ=255)                                                                                     ccGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTc                                                                                                                                       >  1:122026/1‑251 (MQ=255)                                                                                       gattccttttcGCTGCATGGTTGTAACGGTGTCTGGCCAGTGAGCAAGTTTATCCAGAGCGTGGGGCAGCTCGTCAGCGTAAGCATTGATTTTGAGAAACCACTGCGGGATCTTTTTACGTTCTACTTTGGTATCCCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCCCCAGTTGTCCGCAGAAGTCTTCTTATATACCAGGCCTTGTTTATACAGCTCgg                                                                                                                                     <  2:247312/241‑1 (MQ=255)                                                                                         tCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGCGCAGCTCGTCAGCGTAAGCAGTGATTTTGCTAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGCCGTTCGGGCACCAGTTGACCGCAGAAGCCTTCTTATATACCAGGCCTTTTTTATACAGCTCgggg                                                                                                                                   >  2:143970/1‑249 (MQ=255)                                                                                          ccAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTAGGGCACCAGTTGACCGCAGAAGTCTTATTATATACCAGGCCTTTTTTATACAGCTCGGTg                                                                                                                                   >  2:129513/1‑250 (MQ=255)                                                                                                                             aGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGAGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGt                                                                                                >  1:227418/1‑250 (MQ=255)                                                                                                                              gTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTAc                                                                                              <  1:115860/251‑1 (MQ=255)                                                                                                                              gTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTAc                                                                                              >  1:196508/1‑251 (MQ=255)                                                                                                                                    cAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCAATGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTg                                                                                        >  1:222559/1‑251 (MQ=255)                                                                                                                                    cAGTTTATCCAGATCGTTGAGCAGCTCGGCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTg                                                                                        <  1:142446/251‑1 (MQ=255)                                                                                                                                            ccAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTc                                                                                 <  1:282363/250‑1 (MQ=255)                                                                                                                                                       agcagcTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTc                                                                     >  1:31441/1‑251 (MQ=255)                                                                                                                                                         cagcTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGACTCCAGTCAt                                                                   >  1:379761/1‑251 (MQ=255)                                                                                                                                                                     gTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAACTCGCGGCTCCAGTCATAACCAAAGccc                                                        >  1:269364/1‑250 (MQ=255)                                                                                                                                                                                  ttGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTg                                          >  1:372061/1‑251 (MQ=255)                                                                                                                                                                                                        cattACGTTCAACTTGTGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGTCTCCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTATTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCATCATTTTGAGCTGGTTTTTCATATACGCGAt                        <  1:152003/245‑1 (MQ=255)                                                                                                                                                                                                         tttACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCCCCAGTTGACCGCCGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACAACAGCCCAGCATTTTGGGCTGGTTTTTCATATAAGCGCtgttgt                   >  2:168981/1‑251 (MQ=255)                                                                                                                                                                                                          ttACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGAtgttgt                   <  1:52802/250‑1 (MQ=255)                                                                                                                                                                                                                           actgccgcgcCAGCAACAGCCTTCGATAACTTGTTCGTTCGCCAGTACGGTCTGTTCGTTCGGTCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGTTGcc  <  2:193626/244‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           AAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCC  >  NC_000913/674026‑674493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑