Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	47,434	T→C	H63H (CAT→CAC)	kefF →	regulator of KefC‑mediated potassium transport and quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	47,434	0	T	C	95.0%	105.7 / ‑3.7	41	H63H (CAT→CAC)	kefF	regulator of KefC‑mediated potassium transport and quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (25/13);  total (26/15)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTG‑AAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTG  >  NC_000913/47192‑47675                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   gAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGAATCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGc                                                                                                                                                                                                                                             <  1:82843/250‑1 (MQ=255)             acacTTTGTGGGAAAACAAAGGAGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGCTGCAGGGGTACAGCAt                                                                                                                                                                                                                                >  2:341811/1‑251 (MQ=255)               acTTTGTGGGAAAACAAAGGCGTAAGCACGCGGGGTAGCTATGATTCTTAGAATTGATGCGCATCCGTATCCGCATCATTCCCATGCGAAGAAACGGATGCTTGAACAGGCAAGGACGCGGGAAGGCGTCGAAATTCGCTCGCTTTAGCAACTCTATCCTGACGTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATtc                                                                                                                                                                                                                              <  1:16952/251‑1 (MQ=255)                   tgtgGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATtcctcc                                                                                                                                                                                                                          >  1:26772/1‑251 (MQ=255)                        gAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGctcc                                                                                                                                                                                                                     >  1:223893/1‑251 (MQ=255)                           aaCAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGctcctc                                                                                                                                                                                                                   >  1:263899/1‑250 (MQ=255)                               aaGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACt                                                                                                                                                                                                              >  1:93327/1‑251 (MQ=255)                                 ggtgTAATTACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATACTGACATCAATATCGATATTGCAGCCGAGCAGGTGTCGCTGTCTCGAGCCGATCTGATCGTCTGGTAGCACCCGATGCATTGGTACAGCCATCCCCCGCTCCCCAAAtttt                                                                                                                                                                                                            >  2:121531/4‑251 (MQ=255)                                       aTCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCg                                                                                                                                                                                                      <  1:206140/251‑1 (MQ=255)                                                           ttCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGc                                                                                                                                                                                  >  1:294209/1‑251 (MQ=255)                                                             cTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGACAGCACCCGATGCAGTAGTACAGCATTCCTCCACTCCTCAAACTTTGGATCGATAAAGGTTTCTCCCATGGCTg                                                                                                                                                                                >  2:307999/1‑251 (MQ=255)                                                                       aTGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTATTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTGTCTCCCACGGCTGGGCTTACGGt                                                                                                                                                                      >  1:256987/1‑251 (MQ=255)                                                                                  tATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCAc                                                                                                                                                           >  1:14098/1‑251 (MQ=255)                                                                                  tATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACATCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGAGCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTggg                                                                                                                                                                              <  1:52787/232‑1 (MQ=255)                                                                                      cGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGgcg                                                                                                                                                       <  1:367581/251‑1 (MQ=255)                                                                                            atTCCCATGCGAATAAACGGATGCTAAAACAGGCAAGGCCGCTGGAAGGCGACGACATTGGCTCTCTTTATCAACTATATCCTGACTTCAATATCGATATTGCCGCCGAGCGTGACGCGGTGTTTCGCGCCGATCTGATCGTCTGGCAGCACCCGGTTCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGCTAAAGTGTtc                                                                                                                                                                                           >  1:339944/1‑209 (MQ=255)                                                                                              tCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGCCTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTGTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATg                                                                                                                                                <  1:164970/250‑1 (MQ=255)                                                                                                      gAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACAtt                                                                                                                                       >  1:75332/1‑251 (MQ=255)                                                                                                        aTAAACGGATGCTTGAACAGGCTAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTg                                                                                                                                     <  2:26772/251‑1 (MQ=255)                                                                                                          aaaCGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTAGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCAACCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTTGATCGATAAAGTTTTCTCCCACGGCTGGGCTTCCGGTCATGGCCGCACGGCGCTGCCTGGCaaccactttat                                                                                                                                   >  2:33246/1‑242 (MQ=255)                                                                                                                             cAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTGTATCAACTCTAGCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACcggc                                                                                                                <  1:341811/251‑1 (MQ=255)                                                                                                                              aaGGACGCTGGCAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATACTGACTTCAATATCTATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGAACTGAGCGTCTGGCAGCACCCGGTGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGAACAATAAAGTTTTATCCCACGGCAGGGCTTACGGTCAGGTCGGCACGGAGCTGCATGGCTAACCTTTGCTATGGGCGGTgacgtgcggcg                                                                                                               >  2:337162/1‑244 (MQ=255)                                                                                                                                ggACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGGCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACcggcggc                                                                                                             <  1:92058/251‑1 (MQ=255)                                                                                                                                          aGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACcggcggcg                                                                                                            >  1:318995/1‑242 (MQ=255)                                                                                                                                          aGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACCTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACcggcggcg                                                                                                            <  2:318995/242‑1 (MQ=255)                                                                                                                                            gCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCAt                                                                                                 <  1:327743/251‑1 (MQ=255)                                                                                                                                                 gAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTg                                                                                             >  1:258046/1‑250 (MQ=255)                                                                                                                                                            tctTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTG‑AAATTGGTgcgc                                                                                >  1:236921/1‑251 (MQ=255)                                                                                                                                                             ctTTATCAACTCTATCCTGACTTCAATCTCGATATTCCCGCCCAGCAGGAGACGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGCGGTACAGCATTCCTCCGCTCCGCACACTTTGGAACGCTCAAGTTTTTTCCCACGCCTTGTCTTACGGTCGTGGTGGCCCGGCGCTGCATTGCCAACATTTGCTTGGGGCGGTGACGGCCGGCTGCGGAGAAAGCCacatctcactttggggcgg                                                                                >  2:37235/1‑233 (MQ=255)                                                                                                                                                                   cAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTtc                                                                                                                                                                                           <  1:295644/138‑1 (MQ=255)                                                                                                                                                                   cAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTtc                                                                                                                                                                                           >  2:295644/1‑138 (MQ=255)                                                                                                                                                                     aCTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCCAACATTTGCTGTGTGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTG‑CAATTGGTGCGCATCCGGGCt                                                                       >  1:366510/1‑251 (MQ=255)                                                                                                                                                                                aCTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTG‑AAATTGGTGCGCATCCGGGCTTTGATGTGCTg                                                            >  1:205477/1‑251 (MQ=255)                                                                                                                                                                                aCTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCACGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCATCCGCTCCTCAAACTTTAGATCGATCAAGTTTTATACCACGGCTGGGCTTACGGGCATGGCGGCACGGAGATGCATGGAAAACAATTGCTGTGGGCGGTAACAACCGGCGGCGGGGCAAGCCATTTTG‑AAATTGGTGGGCTTCCGAGATTTGATGTGCtc                                                            >  2:233079/1‑250 (MQ=255)                                                                                                                                                                                  ttCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACTGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGAAGACCAGCGGCGGGGAAAGCCCTTTTG‑AAATTTGTGCGCATCCGGGCTTTGATGTTCTGt                                                           >  1:214649/1‑250 (MQ=255)                                                                                                                                                                                                  ccgcagAGAAGGAGACGCTGTCTCGCGCCGATATGATCGTCTGGCAGAACCCGCTGCAGAGGTACCGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGACATGGCGGCACGGCGCTCCATGTAAAACATTGGCTGGGGGCGGTGACGTCCGGCGGCGGGGCAAGCCATTTTGTGAATGGGT‑CGGATCAGGGCTTTTATGTGTTGTCGCACCCGcccctggcg                                          >  2:127209/1‑243 (MQ=255)                                                                                                                                                                                                          caggaggcgtttgcccgcgCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCGGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTG‑AAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAAt                                  <  2:205477/236‑1 (MQ=255)                                                                                                                                                                                                            ggaggCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGGGACGACCGGCGGCGGGGAAAGCCATTTTG‑AAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCCCTACAGGCGGCGGCAATCt                                >  1:38099/1‑251 (MQ=255)                                                                                                                                                                                                                        ccgcgCCGATCTTATCGTCTGGCAGCACCCGATGCCGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTGTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTG‑AAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGa                    <  2:93327/250‑1 (MQ=255)                                                                                                                                                                                                                           cgcCGATCTGATCGTCTGGCAGCACCCGATGAAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATCAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGCAAGCCATTTTG‑AAATTGGTGCGCATACGGGCTATGATGTGCTGTCGACGCCCACACAGGCGACGGCAATCCACTGCGGGCTGGACt                 >  2:186900/1‑251 (MQ=255)                                                                                                                                                                                                                                          cgggCAGCACCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTG‑AAATTGGTGCGCATCCGGGTTTTGATGTGCGGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTg  <  1:246709/249‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   GAATATATGACTACACTTTGTGGGAAAACAAAGGCGTAATCACGCGGGCTACCTATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATGCTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCTGACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTCTGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTG‑AAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTG  >  NC_000913/47192‑47675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑