Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	45,978	C→T	L58L (CTG→TTG)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,978	0	C	T	97.5%	106.9 / NA	41	L58L (CTG→TTG)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/19);  minor base G (0/1);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAA‑ACTGGACGCTGACT‑GGATTGGCTTGCTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACC  >  NC_000913/45731‑46202                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                  tgGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAC‑ACTGGACGCTGACT‑GGACTGGCTTGTTgg                                                                                                                                                                                                                                >  1:44749/1‑251 (MQ=255)          gTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGAAATGATTGGCGTGGCGCTGGAGCAACTGAAGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGATTGTTGGG‑CGCGGGa                                                                                                                                                                                                                       >  1:263409/1‑250 (MQ=255)                              gatgcGTAACGCCGCTCTGATCCTCTTTGAACAAAACAGGAATAACCAGGCAACCGCCCATAAATTTTGCTGATTTCAAAT‑CTCCTTCTAGTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGAGTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGt                                                                                                                                                                                                   <  2:74759/246‑1 (MQ=255)                                          cgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACAGTCCAGCAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGCGCAACTGACGCCGGCGCTAAA‑ACTGGCCGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTGCGTTGGCACAACGCTg                                                                                                                                                                                      >  2:323397/1‑251 (MQ=255)                                           gcACTGACGCGCATTGCAAAAAACAGGAAGAACCATGCAACCTTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCCCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGAGTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGt                                                                                                                                                                                     <  1:359586/251‑1 (MQ=255)                                                      cATTGCAAAAAACAGGAATACCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCtat                                                                                                                                                                             <  1:269695/248‑1 (MQ=255)                                                      cATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GAATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATAtt                                                                                                                                                                          >  1:325273/1‑251 (MQ=255)                                                               aaaCAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATaa                                                                                                                                                                  >  1:308133/1‑250 (MQ=255)                                                                 aCAGGAATACCCATGCACCCGTCCAGAAACTTGGACGACCTCAAATTCTCCT‑CTATTCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGt                                                                                                                                                               <  1:48749/251‑1 (MQ=255)                                                                   aGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGATTGTTGTG‑CGCGGGAACCATCGCCGGGCTGTTCGGTGGCACATAGCTGTTTGGCTATATTTCAGAAAAAGTCg                                                                                                                                                             >  1:204404/1‑251 (MQ=255)                                                                        tAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACAGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGTTTGGCTTGTTGGG‑CGCGGGAACTCTCGCCGGGCTGTTCGTTGGCACATCTCTGTTTGGATATATTTGCGATACAGTCGGAccg                                                                                                                                                        >  2:287166/1‑249 (MQ=255)                                                                         aaCCATGCATCCGCCCAGAAACTTTGTCGAGTTCAAATTCTCCT‑CTATGCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGgc                                                                                                                                                       <  1:2841/251‑1 (MQ=255)                                                                         aaCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGACGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGgc                                                                                                                                                       >  1:369877/1‑251 (MQ=255)                                                                             atgcacccatcaaGAAACTTTGACGATCTCAACTTCTCCC‑CCATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGCATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGTCACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑AGCGGGAACGCTCGCCGGGCTGGTCGTTGGCACAGCGGTGTTTGGATAAATTTCCGATAAAGTCGGACGGCGCa                                                                                                                                                    >  2:27162/13‑250 (MQ=255)                                                                             aTGCAACCGTCCAGAAACTATGACGATCTCAACTTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGATGGAGCAACTGACGCCGGCGATAAA‑ACTGGACGCTGACT‑GGCTTGGCTTGTTGGT‑CGCGGGAACGCTCGCCGGGCCGTTCGTTGGCATATGGCGTTTTGGTTATTTTTCAGATAAGGTCGGGCggagac                                                                                                                                                    >  2:257497/1‑246 (MQ=255)                                                                                cAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAAt                                                                                                                                                >  1:287682/1‑251 (MQ=255)                                                                                        cAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCCAAATGTTCCTc                                                                                                                                         >  1:241728/1‑250 (MQ=255)                                                                                           aaaCTTTGACGATCTCAAATTCTCCT‑CTAATCACCGCCGCATTTTGCTGTGGGGAAGAGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGAAGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTCTCTTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTg                                                                                                                                     >  1:131479/1‑251 (MQ=255)                                                                                             aCTTTGACGACCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGat                                                                                                                                   <  1:219278/251‑1 (MQ=255)                                                                                               tttGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGat                                                                                                                                   <  1:369120/249‑1 (MQ=255)                                                                                               tttGACGATCTCAAATTCTCCT‑ATATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGCTGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGCGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGAGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGTTAAAGTAGGACGGCGCAAAATTTTCATCATTgtt                                                                                                                                   >  2:369120/1‑247 (MQ=255)                                                                                                     gATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGAGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGc                                                                                                                           >  1:170249/1‑251 (MQ=255)                                                                                                               tctcCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTg                                                                                                                 >  1:48203/1‑251 (MQ=255)                                                                                                                 tcct‑cTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGat                                                                                                               >  1:375877/1‑251 (MQ=255)                                                                                                                        aTTCGCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTTAA‑ACTGGACGCTGACT‑GGATTGGCTTGTGGGG‑CGCGGGAACGCTCGCCGTGCTGTTCGTTTGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCAGCGGCGTGATATc                                                                                                            <  1:69850/248‑1 (MQ=255)                                                                                                                                                                  tttgATGGTTATGTTCTGGTACTGATTAGCGTCGCGCTGGAGCATCTTGCGTCTGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGGTGGG‑CTCGGTAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCTGCGTGATATCGGTGGCGACGATGTTTGTTGCATCCCCCGTCGAACTGTTGGtta                                                                <  1:80436/247‑3 (MQ=255)                                                                                                                                                                    ggATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGa                                                                <  1:166716/248‑1 (MQ=255)                                                                                                                                                                       tGGTTATGTACTGGTAATGAGGGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACG‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCAGCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTTGTGATGCgg                                                           <  2:375877/250‑1 (MQ=255)                                                                                                                                                                          ttATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTAc                                                       >  1:34016/1‑251 (MQ=255)                                                                                                                                                                            aTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACtt                                                     <  1:27162/251‑1 (MQ=255)                                                                                                                                                                            aTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACt                                                      <  2:48203/250‑1 (MQ=255)                                                                                                                                                                                        ggATTGGCGTGGCGCTGGAGCAACTGACGCCTGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTc                                         <  1:229464/250‑1 (MQ=255)                                                                                                                                                                                         gATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTc                                         <  1:134701/250‑1 (MQ=255)                                                                                                                                                                                         gATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCGTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACAGCGCTGTTTGGCTATATGTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACTATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTc                                         <  1:173627/250‑1 (MQ=255)                                                                                                                                                                                          gttGGTGTGGCGTTGGAGCAGCTGACGCTGGCGCTAAA‑ACTGGTCCCTGTCT‑GGATTTGCTTGTTGTG‑CGTGGGAACGCTCTCCGGGCGGTTCGTTGGATCATCGCTGTTTTGCTATATTTCTGATAAAGTCGGACGGCGCAAAATGTTCCTCCTTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGTTGTTTGTTTCATCCCCTGTCGACCTGTTGGTGATGCGGGTACTTATCGGCATGGTCAt                                       <  2:186572/250‑1 (MQ=255)                                                                                                                                                                                            gggcgtgtcgCTGGAGCAACTGACGCCGGCGC‑GAATACGTGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGTCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCTGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTTTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCAtag                                     <  2:181411/250‑3 (MQ=255)                                                                                                                                                                                               cgtggtgCTGGTGTAACTGGCGCCGGCGATAAA‑ACTGGAGGC‑GTCTTGTATTGGCTTG‑TGGGTCGCGGTAACGCTCGCCGGGCTGTTCGTTGGCATATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTTATTGATATCATCGCCATCGGCGTGATATCGGTGGCGTCGATTTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTAATCGGCATTGTCATCgttg                                  <  2:281542/251‑4 (MQ=255)                                                                                                                                                                                                                             aaa‑aCTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGATCGCTCGCCGGGCTGTTCGTTGGCATATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGAt      <  1:257497/248‑1 (MQ=255)                                                                                                                                                                                                                             aaa‑aCTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATc     <  1:243622/249‑1 (MQ=255)                                                                                                                                                                                                                             aaa‑aCTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGGTGGTGATGCGGGGACTTATCAGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATc     >  1:158213/2‑250 (MQ=255)                                                                                                                                                                                                                               a‑aCTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCCAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGGGGCGACGATGTTTTTTTAATCCCCCGTCGAAATTGTGGAGTAGCGGGTTATTACCGGCATTGTCATAGGGGCCGATTATCCCACCGCCCCCTCCATGATCAcc  >  2:371647/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                  TGGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAA‑ACTGGACGCTGACT‑GGATTGGCTTGCTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACC  >  NC_000913/45731‑46202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑