Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	45,950	G→A	L48L (CTG→CTA)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,950	0	G	A	97.4%	109.9 / NA	40	L48L (CTG→CTA)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (19/19);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ACCCGCGCGGCACCTTTGGTGTGGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAA‑ACTGGACGCTGACT‑GGATTGGCTTGCTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTG  >  NC_000913/45710‑46170                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                acctgtgcgGCACCTTTGGTGTGGATTTCGGTTCAGGCTGATGTTGTTTTGATACGTAACGCCTCCCTGACTCTCATTGCAAAAAACAGGAATACCCATGCAACCGTCCAATACCTTGGACGTTCTCAAAGTCTCCT‑CTCTTCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACg                                                                                                                                                                                                                      <  2:95600/245‑1 (MQ=255)                  ggTGTGGAGTTCCGTTACGGCTGATTTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCtt                                                                                                                                                                                                     <  1:258055/250‑1 (MQ=255)                  ggTGTGGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTg                                                                                                                                                                                                    >  1:76209/1‑251 (MQ=255)                      tgGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAC‑ACTGGACGCTGACT‑GGACTGGCTTGTTgg                                                                                                                                                                                                >  1:44749/1‑251 (MQ=255)                               gTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGAAATGATTGGCGTGGCGCTGGAGCAACTGAAGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGATTGTTGGG‑CGCGGGa                                                                                                                                                                                       >  1:263409/1‑250 (MQ=255)                                                   gatgcGTAACGCCGCTCTGATCCTCTTTGAACAAAACAGGAATAACCAGGCAACCGCCCATAAATTTTGCTGATTTCAAAT‑CTCCTTCTAGTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGAGTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGt                                                                                                                                                                   <  2:74759/246‑1 (MQ=255)                                                               cgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACAGTCCAGCAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGCGCAACTGACGCCGGCGCTAAA‑ACTGGCCGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTGCGTTGGCACAACGCTg                                                                                                                                                      >  2:323397/1‑251 (MQ=255)                                                                gcACTGACGCGCATTGCAAAAAACAGGAAGAACCATGCAACCTTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCCCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGAGTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGt                                                                                                                                                     <  1:359586/251‑1 (MQ=255)                                                                           cATTGCAAAAAACAGGAATACCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCtat                                                                                                                                             <  1:269695/248‑1 (MQ=255)                                                                           cATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GAATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATAtt                                                                                                                                          >  1:325273/1‑251 (MQ=255)                                                                                    aaaCAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATaa                                                                                                                                  >  1:308133/1‑250 (MQ=255)                                                                                      aCAGGAATACCCATGCACCCGTCCAGAAACTTGGACGACCTCAAATTCTCCT‑CTATTCTCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGt                                                                                                                               <  1:48749/251‑1 (MQ=255)                                                                                        aGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGATTGTTGTG‑CGCGGGAACCATCGCCGGGCTGTTCGGTGGCACATAGCTGTTTGGCTATATTTCAGAAAAAGTCg                                                                                                                             >  1:204404/1‑251 (MQ=255)                                                                                             tAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACAGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGTTTGGCTTGTTGGG‑CGCGGGAACTCTCGCCGGGCTGTTCGTTGGCACATCTCTGTTTGGATATATTTGCGATACAGTCGGAccg                                                                                                                        >  2:287166/1‑249 (MQ=255)                                                                                              aaCCATGCATCCGCCCAGAAACTTTGTCGAGTTCAAATTCTCCT‑CTATGCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGgc                                                                                                                       <  1:2841/251‑1 (MQ=255)                                                                                              aaCCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGACGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGgc                                                                                                                       >  1:369877/1‑251 (MQ=255)                                                                                                  atgcacccatcaaGAAACTTTGACGATCTCAACTTCTCCC‑CCATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGCATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGTCACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑AGCGGGAACGCTCGCCGGGCTGGTCGTTGGCACAGCGGTGTTTGGATAAATTTCCGATAAAGTCGGACGGCGCa                                                                                                                    >  2:27162/13‑250 (MQ=255)                                                                                                  aTGCAACCGTCCAGAAACTATGACGATCTCAACTTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGATGGAGCAACTGACGCCGGCGATAAA‑ACTGGACGCTGACT‑GGCTTGGCTTGTTGGT‑CGCGGGAACGCTCGCCGGGCCGTTCGTTGGCATATGGCGTTTTGGTTATTTTTCAGATAAGGTCGGGCggagac                                                                                                                    >  2:257497/1‑246 (MQ=255)                                                                                                     cAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAAt                                                                                                                >  1:287682/1‑251 (MQ=255)                                                                                                             cAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCCAAATGTTCCTc                                                                                                         >  1:241728/1‑250 (MQ=255)                                                                                                                aaaCTTTGACGATCTCAAATTCTCCT‑CTAATCACCGCCGCATTTTGCTGTGGGGAAGAGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGAAGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTCTCTTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTg                                                                                                     >  1:131479/1‑251 (MQ=255)                                                                                                                  aCTTTGACGACCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGat                                                                                                   <  1:219278/251‑1 (MQ=255)                                                                                                                    tttGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGat                                                                                                   <  1:369120/249‑1 (MQ=255)                                                                                                                    tttGACGATCTCAAATTCTCCT‑ATATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGCTGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGCGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGAGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGTTAAAGTAGGACGGCGCAAAATTTTCATCATTgtt                                                                                                   >  2:369120/1‑247 (MQ=255)                                                                                                                          gATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGAGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGc                                                                                           >  1:170249/1‑251 (MQ=255)                                                                                                                                    tctcCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTg                                                                                 >  1:48203/1‑251 (MQ=255)                                                                                                                                      tcct‑cTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGat                                                                               >  1:375877/1‑251 (MQ=255)                                                                                                                                             aTTCGCCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTTAA‑ACTGGACGCTGACT‑GGATTGGCTTGTGGGG‑CGCGGGAACGCTCGCCGTGCTGTTCGTTTGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCAGCGGCGTGATATc                                                                            <  1:69850/248‑1 (MQ=255)                                                                                                                                                                                       tttgATGGTTATGTTCTGGTACTGATTAGCGTCGCGCTGGAGCATCTTGCGTCTGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGGTGGG‑CTCGGTAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCTGCGTGATATCGGTGGCGACGATGTTTGTTGCATCCCCCGTCGAACTGTTGGtta                                <  1:80436/247‑3 (MQ=255)                                                                                                                                                                                         ggATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGa                                <  1:166716/248‑1 (MQ=255)                                                                                                                                                                                            tGGTTATGTACTGGTAATGAGGGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACG‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCAGCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTTGTGATGCgg                           <  2:375877/250‑1 (MQ=255)                                                                                                                                                                                               ttATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTAc                       >  1:34016/1‑251 (MQ=255)                                                                                                                                                                                                 aTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACtt                     <  1:27162/251‑1 (MQ=255)                                                                                                                                                                                                 aTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACt                      <  2:48203/250‑1 (MQ=255)                                                                                                                                                                                                             ggATTGGCGTGGCGCTGGAGCAACTGACGCCTGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTc         <  1:229464/250‑1 (MQ=255)                                                                                                                                                                                                              gATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTc         <  1:134701/250‑1 (MQ=255)                                                                                                                                                                                                              gATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTAAA‑ACTGGACGCTGACT‑GGATTGGCGTGTTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACAGCGCTGTTTGGCTATATGTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACTATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTc         <  1:173627/250‑1 (MQ=255)                                                                                                                                                                                                               gttGGTGTGGCGTTGGAGCAGCTGACGCTGGCGCTAAA‑ACTGGTCCCTGTCT‑GGATTTGCTTGTTGTG‑CGTGGGAACGCTCTCCGGGCGGTTCGTTGGATCATCGCTGTTTTGCTATATTTCTGATAAAGTCGGACGGCGCAAAATGTTCCTCCTTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGTTGTTTGTTTCATCCCCTGTCGACCTGTTGGTGATGCGGGTACTTATCGGCATGGTCAt       <  2:186572/250‑1 (MQ=255)                                                                                                                                                                                                                 gggcgtgtcgCTGGAGCAACTGACGCCGGCGC‑GAATACGTGACGCTGACT‑GGATTGGCTTGTTGGG‑CGCGGGAACGCTCGCCGGTCTGTTCGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCTGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTTTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCAtag     <  2:181411/250‑3 (MQ=255)                                                                                                                                                                                                                    cgtggtgCTGGTGTAACTGGCGCCGGCGATAAA‑ACTGGAGGC‑GTCTTGTATTGGCTTG‑TGGGTCGCGGTAACGCTCGCCGGGCTGTTCGTTGGCATATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTTATTGATATCATCGCCATCGGCGTGATATCGGTGGCGTCGATTTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTAATCGGCATTGTCATCgttg  <  2:281542/251‑4 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                ACCCGCGCGGCACCTTTGGTGTGGAGTTCCGTTACGGCTGATGTTGGTTTGATACGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCT‑CTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAA‑ACTGGACGCTGACT‑GGATTGGCTTGCTGGG‑CGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTG  >  NC_000913/45710‑46170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑