Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	27,026	C→T	D250D (GAC→GAT)	ispH →	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	27,026	0	C	T	95.7%	113.8 / ‑1.8	46	D250D (GAC→GAT)	ispH	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (15/29);  total (16/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

GTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTT  >  NC_000913/26783‑27268                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   gTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAg                                                                                                                                                                                                                                              <  1:163117/250‑1 (MQ=255)    gggggATGACACGTCTGATGTGATCGACGCGTGGCGTAAACGTGTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGAGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGGCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGACCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAag                                                                                                                                                                                                                                          <  2:237944/248‑1 (MQ=255)           gACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTggg                                                                                                                                                                                                                                    <  1:259971/250‑1 (MQ=255)                   tGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGCGTGGGTGAAAGAgg                                                                                                                                                                                                                           >  1:210957/1‑251 (MQ=255)                    gATGTGATCGACGCTCTGCGTAAACGCGTCCCGAAAATTGGCGGTCCGCGCAAAGATGACACCTGCTACGCCACGACTAACTGTCAGGAAGCGTGACTCGCTCTG‑GCAGAACAGTCGGAAGTTGTGTTGTTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATTTGCAAACGCGCGTTTTTTAGTGACTATGCGAAAGATATCCAGGAACTGTCGGTGAAagag                                                                                                                                                                                                                            <  2:143707/249‑1 (MQ=255)                          aTCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATg                                                                                                                                                                                                                    <  1:314928/251‑1 (MQ=255)                              aCGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGCAGAGTGGGTGAAAGGGGTTAAATGCGTc                                                                                                                                                                                                                >  1:159941/1‑251 (MQ=255)                                 cgcTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGc                                                                                                                                                                                                             <  1:377044/251‑1 (MQ=255)                                  gcTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGATAAATGCggcagcg                                                                                                                                                                                                            >  1:201715/1‑245 (MQ=255)                                   cTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTg                                                                                                                                                                                                                                  <  1:343956/228‑1 (MQ=255)                                                          tGTCGGGCCCCGCAAAGATGTCAGTTGCTTCGACACGTTTAACCGTCAGGAAGCGGTACGCGCCCTG‑GAAGACTAGGCGGACGTTGTGTTGTTGGTCGGTTAGAAAACCTCCTCCAACTCCAACCGTCTGGCGGAGCTGTCCCAGCTTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGGGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCTCATTGGATCCg                                                                                                                                                                                     <  2:254319/250‑1 (MQ=255)                                                                       aaaGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCATG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTc                                                                                                                                                                                                                >  1:231524/1‑210 (MQ=255)                                                                             gACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAAtgt                                                                                                                                                                 <  1:134910/251‑1 (MQ=255)                                                                               cATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATgtgg                                                                                                                                                               <  1:15935/251‑1 (MQ=255)                                                                                    gatacgatgcgccTAACCTTCATGAAGCGGTACGCGCC‑TGTGCACAACAGGCGGAAGTTGTGTTGGGGTTCGGTACTAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTTGCCCCGCGTATGAGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCATGACGAGCGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTTCGGCTATTCTCGTGTAAAATgtggt                                                                                                                                                              <  2:43558/235‑1 (MQ=255)                                                                                        cGCCACGACTAACAGTCAGGAAGCGGTACGCGCCCTG‑GCAGACCAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTACATGCGTCGGAGGGACTGAGGGCGCATTGGCTCCGGATATTCTGGTGCAAAATGTGGTGGcccggt                                                                                                                                                      >  2:346880/1‑246 (MQ=255)                                                                                          ccACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTg                                                                                                                                                    <  1:350203/251‑1 (MQ=255)                                                                                                  aaCCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCt                                                                                                                                            <  1:117807/251‑1 (MQ=255)                                                                                                    ccGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTgg                                                                                                                                          <  1:275438/251‑1 (MQ=255)                                                                                                        cAGGAAGCGGTAAGCGCCCTG‑GCAGACCAGGCGGAAGTTGTGTTGGTGGACGGGTCGAAAAACTCCTCCACCTCCAGCCGTCGGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCTGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGTGCgg                                                                                                                                      <  2:110547/251‑1 (MQ=255)                                                                                                           gAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGGGGGGGCACGTTTGCAGCAGCTGGGCggtgg                                                                                                                                   >  1:233145/1‑251 (MQ=255)                                                                                                           gAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGGGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGACAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGATTGCAGCAGCTGGGCggtgg                                                                                                                                   >  1:198263/1‑251 (MQ=255)                                                                                                           gAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTGGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTAAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCggtgg                                                                                                                                   <  2:181098/251‑1 (MQ=255)                                                                                                                ggTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAg                                                                                                                              <  1:295990/251‑1 (MQ=255)                                                                                                                 gTACGCGCCCTG‑GCAGAACAGGTGGAAGTTGTGTTGTTTGTCTGTTCGAAAAACACCTCCAACTCCAACCGGCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGGCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGGGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGc                                                                                                                             <  2:321567/251‑1 (MQ=255)                                                                                                                       agccctgtcatatcagtcGGAAGTTTGGTTGGTTGTTGGTTCTAAAAACTCCTCCAACTCCTATCGTCTTGCGGAGCTGGTCCAGCGTATGGTCAAACGTGTGTTTTTGATTGATGATGCTAAAGATAGCCAGGAAGAGTGGGTGAAAGAGGTTAAATTCGTCGGCGTTTCTGCTGGCGCATCGGCTCCGGATAGTCTGGTGCAAAATTTGGTGGTACGTTTGCAGCAGATTGTCGGTGTTGAATCCAtt                                                                                                                         <  2:366498/233‑1 (MQ=255)                                                                                                                                    cAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGATGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCg                                                                                                           <  1:19015/251‑1 (MQ=255)                                                                                                                                           aaGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGTGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTgaaga                                                                                                     >  1:236478/1‑250 (MQ=255)                                                                                                                                                      gtggtCGGCTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGAGGGTGAAAGAGGTTAACTGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGATGCAAAATGGGGTGGCACGTTTGCAGCAGCTGGGCGGAGGGGAAGCACTTACGCTGGAAGGCCGTGCAGAAAACATTGattt                                                                                         >  2:373650/1‑251 (MQ=255)                                                                                                                                                       gggtCGGTTCGAAAAAACCCTCCAACCCCAACCGTCTGGCGGAGCTGGCCCTGCGTATGTGCAAACGCGCGTTTTTGATTGACGAGGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCGGGAAGGCCGTGAAGAAAACATTGtttgc                                                                                        <  2:147590/250‑3 (MQ=255)                                                                                                                                                        ggtCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCg                                                                                       <  1:152971/251‑1 (MQ=255)                                                                                                                                                                cGAAAAACTCCTCCCACTCCAACCGTCAGGCGGCGCTGGCCCAGAGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGACAGATATCCAGGAAGCGTGGGGGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCTCATCGGCTCCGGATATTCTGGTGCAAAATGGGGGGGACCGTTAGAAGCAGCTGGGCGGTGGTGAATCCATTTCGATGGAAGGACGTGAAGAAACCATTTTTTTCGATGTGc                                                                                 >  2:227771/1‑249 (MQ=255)                                                                                                                                                                cGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCg                                                                               <  1:32282/251‑1 (MQ=255)                                                                                                                                                                         cctccAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATACAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCt                                                                       <  1:382130/250‑1 (MQ=255)                                                                                                                                                                          ctccaactccaaCCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGACGAAAGAGCTGc                                                                     >  1:230093/1‑251 (MQ=255)                                                                                                                                                                             caactcaaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGCAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGAGACTGCGGGCGCATCGTCTCCGGATATTCTGGTGCAAAATGAGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGGAGCAATTCCGCTGGAAGGCAGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCgtg                                                                  >  2:26534/1‑251 (MQ=255)                                                                                                                                                                                      ccGTCTGGCGGAGCTGGCTGAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATAGCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCTGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGTTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTc                                                         <  2:206408/251‑1 (MQ=255)                                                                                                                                                                                      ccGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTc                                                         <  1:346880/251‑1 (MQ=255)                                                                                                                                                                                              cGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGGGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGATAGAGCTGCGTGTCGATATTCGTGAAGTc                                                 <  1:227771/251‑1 (MQ=255)                                                                                                                                                                                                     ggCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTaa                                           <  1:259824/250‑1 (MQ=255)                                                                                                                                                                                                     ggCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTa                                            >  1:349838/1‑249 (MQ=255)                                                                                                                                                                                                       cccAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTc                                        >  1:197852/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCGAGGCGAAAGATATCCAGGAGGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAATGCCGTGAAGAAAACATTGTTTTCGAAGTGCTGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTc       <  1:59984/251‑1 (MQ=255)                                                                                                                                                                                                                                         cGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTGTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGAGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATAGGTTTCGAAGGGCCGCCAGATCTGCGTGTCGTTGTTCGTGAAGTAGTTTAAGTCCTTAACAGCCTACGTTATGCGAAAATGCCAGTCt      >  2:3836/1‑251 (MQ=255)                                                                                                                                                                                                                                           aggCGAAAGATAGCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTg    <  2:210299/249‑1 (MQ=255)                                                                                                                                                                                                                                             gCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGtt  >  1:180979/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   GTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTG‑GCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTT  >  NC_000913/26783‑27268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑